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Structural Basis for a Safety-Belt Mechanism That Anchors Condensin to Chromosomes.

Abstract：

:Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin rearrangements that take place during cell divisions. Here, we identify a direct DNA-binding site in the eukaryotic condensin complex, which is formed by its Ycg1Cnd3 HEAT-repeat and Brn1Cnd2 kleisin subunits. DNA co-crystal structures reveal a conserved, positively charged groove that accommodates the DNA double helix. A peptide loop of the kleisin subunit encircles the bound DNA and, like a safety belt, prevents its dissociation. Firm closure of the kleisin loop around DNA is essential for the association of condensin complexes with chromosomes and their DNA-stimulated ATPase activity. Our data suggest a sophisticated molecular basis for anchoring condensin complexes to chromosomes that enables the formation of large-sized chromatin loops.

journal_name

Cell

journal_title

Cell

authors

Kschonsak M,Merkel F,Bisht S,Metz J,Rybin V,Hassler M,Haering CH

doi

10.1016/j.cell.2017.09.008

subject

Has Abstract

pub_date

2017-10-19 00:00:00

pages

588-600.e24

issue

3

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(17)31057-7

journal_volume

171

pub_type

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