Abstract：
:The phage Mu transpososome is assembled by interactions of transposase subunits with the left (L) and right (R) ends of Mu and an enhancer (E) located in between. A metastable three-site complex LER progresses into a more stable type 0 complex in which a tetrameric transposase is poised for DNA cleavage. "Difference topology" has revealed five trapped negative supercoils within type 0, three contributed by crossings of E with L and R, and two by crossings of L with R. This is the most complex DNA arrangement seen to date within a recombination synapse. Contrary to the prevailing notion, the enhancer appears not to be released immediately following type 0 assembly. Difference topology provides a simple method for determining the ordered sequestration of DNA segments within nucleoprotein assemblies.
journal_name
Celljournal_title
Cellauthors
Pathania S,Jayaram M,Harshey RMdoi
10.1016/s0092-8674(02)00728-6subject
Has Abstractpub_date
2002-05-17 00:00:00pages
425-36issue
4eissn
0092-8674issn
1097-4172pii
S0092867402007286journal_volume
109pub_type
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