Abstract:
:Although clonal neo-antigen burden is associated with improved response to immune therapy, the functional basis for this remains unclear. Here we study this question in a novel controlled mouse melanoma model that enables us to explore the effects of intra-tumor heterogeneity (ITH) on tumor aggressiveness and immunity independent of tumor mutational burden. Induction of UVB-derived mutations yields highly aggressive tumors with decreased anti-tumor activity. However, single-cell-derived tumors with reduced ITH are swiftly rejected. Their rejection is accompanied by increased T cell reactivity and a less suppressive microenvironment. Using phylogenetic analyses and mixing experiments of single-cell clones, we dissect two characteristics of ITH: the number of clones forming the tumor and their clonal diversity. Our analysis of melanoma patient tumor data recapitulates our results in terms of overall survival and response to immune checkpoint therapy. These findings highlight the importance of clonal mutations in robust immune surveillance and the need to quantify patient ITH to determine the response to checkpoint blockade.
journal_name
Celljournal_title
Cellauthors
Wolf Y,Bartok O,Patkar S,Eli GB,Cohen S,Litchfield K,Levy R,Jiménez-Sánchez A,Trabish S,Lee JS,Karathia H,Barnea E,Day CP,Cinnamon E,Stein I,Solomon A,Bitton L,Pérez-Guijarro E,Dubovik T,Shen-Orr SS,Miller ML,Medoi
10.1016/j.cell.2019.08.032subject
Has Abstractpub_date
2019-09-19 00:00:00pages
219-235.e21issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(19)30951-1journal_volume
179pub_type
杂志文章相关文献
CELL文献大全abstract::Autophagy has been associated with both cell survival and cell death, but the role of autophagy in cell death has been controversial. In this issue, Berry and Baehrecke (2007) report that autophagy is involved in physiological cell death during Drosophila development and is controlled by similar mechanisms as those th...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.11.027
更新日期:2007-12-14 00:00:00
abstract::The retinoblastoma tumor suppressor protein (RB) binds several cellular proteins involved in cell cycle progression. Using the yeast two-hybrid system, we found that RB bound specifically to the protein BRG1. BRG1 shares extensive sequence similarity to Drosophila brahma, an activator of homeotic gene expression, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90405-7
更新日期:1994-10-07 00:00:00
abstract::Meiotic cohesin serves in sister chromatid linkage and DNA repair until its subunit Rec8 is cleaved by separase. Separase is activated when its inhibitor, securin, is polyubiquitinated by the Cdc20 regulated anaphase-promoting complex (APC(Cdc20)) and consequently degraded. Differently regulated APCs (APC(Cdh1), APC(A...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.01.017
更新日期:2005-03-25 00:00:00
abstract::The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90549-5
更新日期:1994-11-18 00:00:00
abstract::cDNA clones of the Drosophila epidermal growth factor receptor homolog (DER) gene were isolated and sequenced. The deduced amino acid sequence shows a similar degree of homology to the human epidermal growth factor receptor and to the rat and human neu proteins; the most striking difference is the addition of a third ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90709-9
更新日期:1986-09-26 00:00:00
abstract::Immune evasion is a hallmark of cancer. Losing the ability to present neoantigens through human leukocyte antigen (HLA) loss may facilitate immune evasion. However, the polymorphic nature of the locus has precluded accurate HLA copy-number analysis. Here, we present loss of heterozygosity in human leukocyte antigen (L...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.10.001
更新日期:2017-11-30 00:00:00
abstract::During development, neurons switch among growth states, such as initial axon outgrowth, axon pruning, and regrowth. By studying the stereotypic remodeling of the Drosophila mushroom body (MB), we found that the heme-binding nuclear receptor E75 is dispensable for initial axon outgrowth of MB γ neurons but is required ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.11.047
更新日期:2016-01-14 00:00:00
abstract::The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.03.035
更新日期:2020-04-16 00:00:00
abstract::Due to the rapid emergence of antibiotic-resistant bacteria, there is a growing need to discover new antibiotics. To address this challenge, we trained a deep neural network capable of predicting molecules with antibacterial activity. We performed predictions on multiple chemical libraries and discovered a molecule fr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.01.021
更新日期:2020-02-20 00:00:00
abstract::AU-rich elements (AREs), present in mRNA 3'-UTRs, are potent posttranscriptional regulatory signals that can rapidly effect changes in mRNA stability and translation, thereby dramatically altering gene expression with clinical and developmental consequences. In human cell lines, the TNFalpha ARE enhances translation r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.01.038
更新日期:2007-03-23 00:00:00
abstract::In female mammals a "random choice" mechanism decides which of the two X chromosomes will be inactivated. It has been postulated that Xist is crucial for heterochromatinization and thus functions downstream of the choice mechanism. Here we report that females heterozygous for an internal deletion in the Xist gene, whi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81133-2
更新日期:1998-03-06 00:00:00
abstract::In C. elegans, removing the germ cells extends life span by triggering the nuclear localization and activation of the DAF-16/FOXO transcription factor in the intestine. In this study, we identify and analyze genes required for germline removal to extend life span. We find that the reproductive system communicates with...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.01.039
更新日期:2006-03-10 00:00:00
abstract::Excessive accumulation of neurofilaments in the cell bodies and proximal axons of motor neurons is a major pathological hallmark during the early stages of many human motor neuron diseases. To test directly the consequence of overexpression of the major neurofilament subunit NF-L, we produced transgenic mice that accu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90157-l
更新日期:1993-04-09 00:00:00
abstract::We describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many sub-cellular components (synapses, synaptic vesicles, spines, spi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.06.054
更新日期:2015-07-30 00:00:00
abstract::The genetics of complex disease produce alterations in the molecular interactions of cellular pathways whose collective effect may become clear through the organized structure of molecular networks. To characterize molecular systems associated with late-onset Alzheimer's disease (LOAD), we constructed gene-regulatory ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.03.030
更新日期:2013-04-25 00:00:00
abstract::To understand the role of FoxO family members in hematopoiesis, we conditionally deleted FoxO1, FoxO3, and FoxO4 in the adult hematopoietic system. FoxO-deficient mice exhibited myeloid lineage expansion, lymphoid developmental abnormalities, and a marked decrease of the lineage-negative Sca-1+, c-Kit+ (LSK) compartme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.01.003
更新日期:2007-01-26 00:00:00
abstract::Behavioral expression of food-associated memory in fruit flies is constrained by satiety and promoted by hunger, suggesting an influence of motivational state. Here, we identify a neural mechanism that integrates the internal state of hunger and appetitive memory. We show that stimulation of neurons that express neuro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.035
更新日期:2009-10-16 00:00:00
abstract::Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesives selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90222-z
更新日期:1990-04-06 00:00:00
abstract::Proteins with dibasic retention motifs are subject to retrograde transport to endoplasmic reticulum (ER) by COPI-coated vesicles. As forward transport requires escape from ER retention, general release mechanisms have been expected. Here, KCNK3 potassium channels are shown to bear two cytoplasmic trafficking motifs: a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01040-1
更新日期:2002-11-15 00:00:00
abstract::We have analyzed the transcriptional regulation of plastid genes during chloroplast development in illuminated spinach cotyledons and during leaf formation. The RNAs encoded by plastid genes accumulate with different kinetics during the developmental transitions. Using a novel plastid run-on transcription assay we dem...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90290-x
更新日期:1987-05-08 00:00:00
abstract::Activation of Agouti-related peptide (AgRP) neurons potently promotes feeding, and chronically altering their activity also affects peripheral glucose homeostasis. We demonstrate that acute activation of AgRP neurons causes insulin resistance through impairment of insulin-stimulated glucose uptake into brown adipose t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.02.044
更新日期:2016-03-24 00:00:00
abstract::Autophagy (literally self-eating) has diverse cytoprotective functions but also is linked to programmed cell death (PCD). In this issue, Qu et al. (2007) analyze the role of autophagy in PCD during development. Unexpectedly they find that autophagy induces apoptotic cells to present a signal to ensure their clearance,...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2007.02.023
更新日期:2007-03-09 00:00:00
abstract::Understanding how potent neutralizing antibodies (NAbs) inhibit SARS-CoV-2 is critical for effective therapeutic development. We previously described BD-368-2, a SARS-CoV-2 NAb with high potency; however, its neutralization mechanism is largely unknown. Here, we report the 3.5-Å cryo-EM structure of BD-368-2/trimeric-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.035
更新日期:2020-11-12 00:00:00
abstract::The crystal structure of the β2-adrenergic receptor (β2AR) bound to the G protein adenylyl cyclase stimulatory G protein (Gs) captured the complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, the β2AR-Gsempty complex does not provide a clear explanation for G protein coupling specificity. Evidence from se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.021
更新日期:2019-05-16 00:00:00
abstract::Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.06.036
更新日期:2006-07-28 00:00:00
abstract::To determine the special feature of ribosomal RNA promoters that might account for the highly efficient and regulated synthesis of rRNA in E. coli, we have analyzed the beginnings of two ribosomal RNA operons, rrnD and rrnX. DNA sequences for 425 bp preceding those specifying mature 16s rRNA are reported. In vitro tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90310-6
更新日期:1979-05-01 00:00:00
abstract::From the approximately 1,200 odorant receptor (OR) genes in the mouse genome, an olfactory sensory neuron is thought to express only one gene. The mechanisms of OR gene choice are not understood. A 2.1 kilobase region (the H element) adjacent to a cluster of seven OR genes has been proposed as a trans- and pan-enhance...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.06.023
更新日期:2007-07-27 00:00:00
abstract::Recent work has identified a cascade of membrane bound protein kinases in Ehrlich ascites tumor cells. These enzymes, designated PKL, PKS and PKM, are present in both Ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. We have now purified a fourth protein kinase, PKF, that is also assoc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90227-0
更新日期:1981-07-01 00:00:00
abstract::During asymmetric cell divisions, mitotic spindles align along the axis of polarization. In invertebrates, spindle positioning requires Pins or related proteins and a G protein alpha subunit. A mammalian Pins, called LGN, binds Galphai and also interacts through an N-terminal domain with the microtubule binding protei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.10.028
更新日期:2004-11-12 00:00:00
abstract::This year's Albert Lasker Basic Medical Research Award honors David Allis and Michael Grunstein for their pioneering research that highlighted the importance of histones and their post-translational modifications in the direct control of gene expression. ...
journal_title:Cell
pub_type: 传,历史文章,新闻
doi:10.1016/j.cell.2018.08.032
更新日期:2018-09-20 00:00:00