Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices.

Abstract:

:The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).

journal_name

Cell

journal_title

Cell

authors

Ogata K,Morikawa S,Nakamura H,Sekikawa A,Inoue T,Kanai H,Sarai A,Ishii S,Nishimura Y

doi

10.1016/0092-8674(94)90549-5

subject

Has Abstract

pub_date

1994-11-18 00:00:00

pages

639-48

issue

4

eissn

0092-8674

issn

1097-4172

pii

0092-8674(94)90549-5

journal_volume

79

pub_type

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