Abstract:
:The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be a recognition helix. R2 and R3 are closely packed in the major groove, so that the two recognition helices contact each other directly to bind to the specific base sequence, AACNG cooperatively; this is a significant arrangement of recognition helices. The three key base pairs in this sequence are specifically recognized by Asn-183 (R3), Lys-182 (R3), and Lys-128 (R2). In contrast, R1 has no specific interactions with DNA from our NMR study of the DNA complex of the full DNA-binding domain (R1R2R3).
journal_name
Celljournal_title
Cellauthors
Ogata K,Morikawa S,Nakamura H,Sekikawa A,Inoue T,Kanai H,Sarai A,Ishii S,Nishimura Ydoi
10.1016/0092-8674(94)90549-5subject
Has Abstractpub_date
1994-11-18 00:00:00pages
639-48issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(94)90549-5journal_volume
79pub_type
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