Abstract：
:Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm and thus can be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (quantitative analysis of sacculus architecture remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertions into the outer face than the inner face. Furthermore, crvA is quorum regulated, and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis.
journal_name
Celljournal_title
Cellauthors
Bartlett TM,Bratton BP,Duvshani A,Miguel A,Sheng Y,Martin NR,Nguyen JP,Persat A,Desmarais SM,VanNieuwenhze MS,Huang KC,Zhu J,Shaevitz JW,Gitai Zdoi
10.1016/j.cell.2016.12.019subject
Has Abstractpub_date
2017-01-12 00:00:00pages
172-185.e15issue
1-2eissn
0092-8674issn
1097-4172pii
S0092-8674(16)31735-4journal_volume
168pub_type
杂志文章相关文献
CELL文献大全abstract：:Inconsistent with the view that hair follicle stem cells reside in the matrix area of the hair bulb, we found that label-retaining cells exist exclusively in the bulge area of the mouse hair follicle. The bulge consists of a subpopulation of outer root sheath cells located in the midportion of the follicle at the arre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90696-c
更新日期：1990-06-29 00:00:00
abstract：:During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dyna...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.002
更新日期：2011-11-23 00:00:00
abstract：:Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90037-0
更新日期：1984-04-01 00:00:00
abstract：:To defend cells against viruses, the MAVS (mitochondrial antiviral signaling) adaptor protein initiates an antiviral signaling cascade from mitochondrial membranes. In this issue, Dixit et al. (2010) show that MAVS also localizes to the membranes of peroxisomes, where it rapidly induces expression of a subset of antiv...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.04.043
更新日期：2010-05-14 00:00:00
abstract：:Four major receptor families enable cells to respond to chemical and physical signals from their proximal environment. The ligand- and voltage-gated ion channels, G-protein-coupled receptors, nuclear hormone receptors, and receptor tyrosine kinases are all allosteric proteins that carry multiple, spatially distinct, y...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.08.015
更新日期：2016-08-25 00:00:00
abstract：:A distinct subset of sensory neurons are thought to directly sense changes in thermal energy through their termini in the skin. Very little is known about the molecules that mediate thermoreception by these neurons. Vanilloid Receptor 1 (VR1), a member of the TRP family of channels, is activated by noxious heat. Here ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00652-9
更新日期：2002-03-08 00:00:00
abstract：:A cDNA library has been constructed from an RNA preparation highly enriched in silkmoth chorion mRNAs. Many distinct clones have been identified from this library using a stepwise procedure: scoring for infrequent hexanucleotide restriction enzyme recognition sequences; detailed characterization with restriction enzym...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90241-1
更新日期：1979-12-01 00:00:00
abstract：:The transport kinetics of the influenza virus hemagglutinin from its site of synthesis to the apical plasma membrane of Madin-Darby canine kidney cells, a polarized epithelial cell line, were studied by a sensitive tryptic assay. Hemagglutinin acquired terminal sugars, as judged by sensitivity to endo-beta-N-acetylglu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90154-x
更新日期：1983-08-01 00:00:00
abstract：:Metabolic activity is intimately linked to T cell fate and function. Using high-resolution mass spectrometry, we generated dynamic metabolome and proteome profiles of human primary naive T cells following activation. We discovered critical changes in the arginine metabolism that led to a drop in intracellular L-argini...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.031
更新日期：2016-10-20 00:00:00
abstract：:The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is dete...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90145-8
更新日期：1981-02-01 00:00:00
abstract：:Apoptosis and autophagy are both tightly regulated biological processes that play a central role in tissue homeostasis, development, and disease. The anti-apoptotic protein, Bcl-2, interacts with the evolutionarily conserved autophagy protein, Beclin 1. However, little is known about the functional significance of thi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.07.002
更新日期：2005-09-23 00:00:00
abstract：:The genetics of complex disease produce alterations in the molecular interactions of cellular pathways whose collective effect may become clear through the organized structure of molecular networks. To characterize molecular systems associated with late-onset Alzheimer's disease (LOAD), we constructed gene-regulatory ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.03.030
更新日期：2013-04-25 00:00:00
abstract：:Chimeric histocompatibility genes encoding the amino-terminal (beta 1) domain of the class II Ak beta polypeptide and the carboxy-terminal (C2, transmembrane, and intracytoplasmic) domains of either the class I H-2Ld or H-2Dd molecules were stably introduced into mouse L cells. Although both were transcribed, only 5' ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90139-4
更新日期：1985-02-01 00:00:00
abstract：:The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse pri...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)82000-0
更新日期：1996-11-29 00:00:00
abstract：:cDNA clones of the Drosophila epidermal growth factor receptor homolog (DER) gene were isolated and sequenced. The deduced amino acid sequence shows a similar degree of homology to the human epidermal growth factor receptor and to the rat and human neu proteins; the most striking difference is the addition of a third ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90709-9
更新日期：1986-09-26 00:00:00
abstract：:Constitutive cell-autonomous immunity in metazoans predates interferon-inducible immunity and comprises primordial innate defense. Phagocytes mobilize interferon-inducible responses upon engagement of well-characterized signaling pathways by pathogen-associated molecular patterns (PAMPs). The signals controlling deplo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.09.034
更新日期：2017-11-02 00:00:00
abstract：:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90343-x
更新日期：1984-03-01 00:00:00
abstract：:The pair-rule segmentation gene hairy (h) is expressed and required in alternate metameres in the early Drosophila embryo. We show that h expression outside these domains, driven by an hsp70 promoter, suppresses the expression of fushi tarazu (ftz). The kinetics of action favor h acting directly as a transcriptional r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90636-2
更新日期：1987-11-06 00:00:00
abstract：:The S. pombe nuc2+ gene is required for mitotic chromosome disjunction. Its mutation arrests mitosis at the metaphase. The gene product is present in the nuclear scaffold-like fraction. The nuc2+ protein contains a domain, separated from ten 34 amino acid repeat segments, that is capable of binding AT-rich DNA in vitr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90746-2
更新日期：1990-01-26 00:00:00
abstract：:Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae. Here, we present evidence that Ste5 forms a multikinase complex that joins these kinases for efficient Fus3 activation. B...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90427-8
更新日期：1994-08-12 00:00:00
abstract：:Genetic screening identifies the atypical tetraspanin TM4SF1 as a strong mediator of metastatic reactivation of breast cancer. Intriguingly, TM4SF1 couples the collagen receptor tyrosine kinase DDR1 to the cortical adaptor syntenin 2 and, hence, to PKCα. The latter kinase phosphorylates and activates JAK2, leading to ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.06.009
更新日期：2016-06-30 00:00:00
abstract：:E-cadherin is a major homophilic cell-cell adhesion molecule that inhibits motility of individual cells on matrix. However, its contribution to migration of cells through cell-rich tissues is less clear. We developed an in vivo sensor of mechanical tension across E-cadherin molecules, which we combined with cell-type-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.03.045
更新日期：2014-05-22 00:00:00
abstract：:We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90295-7
更新日期：1983-04-01 00:00:00
abstract：:It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 phys...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.06.028
更新日期：2014-08-14 00:00:00
abstract：:Mutations in gurken and torpedo cause a ventralization in the follicle cell epithelium during Drosophila oogenesis and in the pattern of the embryo that develops in the resultant egg. Both genes lie midway in an epistatic series between fs(1)K10 and dorsal; the mutations block the dorsalization normally observed in K1...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90546-0
更新日期：1987-06-05 00:00:00
abstract：:The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81517-2
更新日期：1999-09-17 00:00:00
abstract：:During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the fla...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90073-4
更新日期：1979-10-01 00:00:00
abstract：:The subcellular localization of the mouse Ltk transmembrane protein tyrosine kinase was studied in transfected COS cells, a mature B lymphocyte line, and a low expressing transfected lymphocyte clone. Indirect immunofluorescence and immunogold staining of COS transfectants and endoglycosidase analysis of both COS tran...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90114-e
更新日期：1991-08-23 00:00:00
abstract：:In this issue, Frey and Görlich (2007) provide new insight into the selective barrier that controls protein traffic through the nuclear pore complex. They show that a single protein domain of the nuclear pore protein Nsp1 can form a hydrogel that allows highly selective access of nuclear transport receptors and their ...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2007.07.029
更新日期：2007-08-10 00:00:00
abstract：:An attenuated form of familial adenomatous polyposis coli, AAPC, causes relatively few colonic polyps, but still carries a significant risk of colon cancer. The mutant alleles responsible for this attenuated phenotype have been mapped in several families to the adenomatous polyposis coli (APC) locus on human chromosom...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90538-2
更新日期：1993-12-03 00:00:00
