Recognition of polyadenylate RNA by the poly(A)-binding protein.

abstract：:Cancer immunotherapy can induce long lasting responses in patients with metastatic cancers of a wide range of histologies. Broadening the clinical applicability of these treatments requires an improved understanding of the mechanisms limiting cancer immunotherapy. The interactions between the immune system and cancer ...

journal_title：Cell

authors： Sharma P,Hu-Lieskovan S,Wargo JA,Ribas A

更新日期：2017-02-09 00:00:00

abstract：:Transcription termination by bacterial RNA polymerase (RNAP) occurs at sequences coding for a GC-rich RNA hairpin followed by a U-rich tract. We used single-molecule techniques to investigate the mechanism by which three representative terminators (his, t500, and tR2) destabilize the elongation complex (EC). For his a...

journal_title：Cell

authors： Larson MH,Greenleaf WJ,Landick R,Block SM

更新日期：2008-03-21 00:00:00

abstract：:The single most frequent cancer-causing mutation across all heterotrimeric G proteins is R201C in Gαs. The current model explaining the gain-of-function activity of the R201 mutations is through the loss of GTPase activity and resulting inability to switch off to the GDP state. Here, we find that the R201C mutation ca...

journal_title：Cell

authors： Hu Q,Shokat KM

更新日期：2018-05-17 00:00:00

abstract：:Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin r...

journal_title：Cell

authors： Kschonsak M,Merkel F,Bisht S,Metz J,Rybin V,Hassler M,Haering CH

更新日期：2017-10-19 00:00:00

abstract：:Receptor-mediated endocytosis of specific ligands is mediated through clustering of receptor-ligand complexes in coated pits on the cell surface. Following this clustering event, the ligand is internalized into a noncoated intracellular vesicle, the receptosome, which selectively avoids fusion with lysosomes and moves...

journal_title：Cell

authors： Willingham MC,Pastan I

更新日期：1980-08-01 00:00:00

abstract：:Phosphoinositide 3 kinase (PI3K) is a key signaling enzyme implicated in receptor-stimulated mitogenesis, oxidative bursting in neutrophils, membrane ruffling, and glucose uptake. A PI3K has already been purified, cloned, and shown to be regulated by receptors that act via tyrosine kinase-dependent regulatory mechanis...

journal_title：Cell

authors： Stephens L,Smrcka A,Cooke FT,Jackson TR,Sternweis PC,Hawkins PT

更新日期：1994-04-08 00:00:00

abstract：:The susceptibility to repair of particular base mismatches by the hex system of Streptococcus pneumoniae was examined by comparison of the nucleotide sequence of the wild-type and eight mutant alleles of the malM gene. A detailed restriction map was constructed for pLS70, and the nucleotide sequence was determined for...

journal_title：Cell

authors： Lacks SA,Dunn JJ,Greenberg B

更新日期：1982-12-01 00:00:00

abstract：:We have characterized regulation of a complex Chlamydomonas reinhardtii chloroplast (PA) whose activity is stimulated by the DNA gyrase inhibitor novobiocin, both in the alga itself and in a heterologous Escherichia coli plasmid system. Since novobiocin is known to reduce torsional stress in E. coli DNA, we interpret ...

journal_title：Cell

authors： Thompson RJ,Mosig G

更新日期：1987-01-30 00:00:00

abstract：:The immortal strand hypothesis posits that the propensity of stem cell compartments to give rise to cancer in later life can be minimized if stem cells, during the process of self-renewal, retain those DNA strands with the fewest mutations acquired during DNA replication. In this Essay, I explore evidence in support o...

journal_title：Cell

authors： Rando TA

更新日期：2007-06-29 00:00:00

abstract：:Translation of ribosomal proteins in the alpha operon of E. coli is repressed by one of the encoded proteins, S4; it specifically recognizes an RNA fragment containing the translational initiation site for the first gene in the operon. RNA structure mapping experiments have suggested a pseudoknot structure for the S4 ...

journal_title：Cell

authors： Tang CK,Draper DE

更新日期：1989-05-19 00:00:00

abstract：:Neurotrophic factors are essential for neuronal survival and function. Recent data have demonstrated that the product of the tyrosine kinase trk proto-oncogene binds NGF and is a component of the high affinity NGF receptor. Analysis of the trkB gene product, gp145trkB, in NIH 3T3 cells indicates that this tyrosine kin...

journal_title：Cell

authors： Soppet D,Escandon E,Maragos J,Middlemas DS,Reid SW,Blair J,Burton LE,Stanton BR,Kaplan DR,Hunter T,Nikolics K,Parada LF

更新日期：1991-05-31 00:00:00

abstract：:The sites at which topoisomerase I interacts with the transcriptionally active ribosomal chromatin of Xenopus oocytes were mapped by treating oocyte nuclei first with camptothecin to stabilize topoisomerase I-rDNA reaction intermediates, and then with SDS to resolve these adducts as protein-linked nicks in the DNA. St...

journal_title：Cell

authors： Culotta V,Sollner-Webb B

更新日期：1988-02-26 00:00:00

abstract：:Metabolic coordination between neurons and astrocytes is critical for the health of the brain. However, neuron-astrocyte coupling of lipid metabolism, particularly in response to neural activity, remains largely uncharacterized. Here, we demonstrate that toxic fatty acids (FAs) produced in hyperactive neurons are tran...

journal_title：Cell

authors： Ioannou MS,Jackson J,Sheu SH,Chang CL,Weigel AV,Liu H,Pasolli HA,Xu CS,Pang S,Matthies D,Hess HF,Lippincott-Schwartz J,Liu Z

更新日期：2019-05-30 00:00:00

abstract：:Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the...

journal_title：Cell

authors： Downey M,Houlsworth R,Maringele L,Rollie A,Brehme M,Galicia S,Guillard S,Partington M,Zubko MK,Krogan NJ,Emili A,Greenblatt JF,Harrington L,Lydall D,Durocher D

更新日期：2006-03-24 00:00:00

abstract：:32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...

journal_title：Cell

authors： Klootwijk J,Klein I,Zabel P,van Kammen A

更新日期：1977-05-01 00:00:00

abstract：:In developing organisms, spatially prescribed cell identities are thought to be determined by the expression levels of multiple genes. Quantitative tests of this idea, however, require a theoretical framework capable of exposing the rules and precision of cell specification over developmental time. We use the gap gene...

journal_title：Cell

authors： Petkova MD,Tkačik G,Bialek W,Wieschaus EF,Gregor T

更新日期：2019-02-07 00:00:00

abstract：:The extracellular space (ECS) of the brain has an extremely complex spatial organization, which has defied conventional light microscopy. Consequently, despite a marked interest in the physiological roles of brain ECS, its structure and dynamics remain largely inaccessible for experimenters. We combined 3D-STED micros...

journal_title：Cell

authors： Tønnesen J,Inavalli VVGK,Nägerl UV

更新日期：2018-02-22 00:00:00

abstract：:We have isolated a conditional lethal mutant of bacteriophage 12 which makes plaques only on E. coli strains carrying a UGA suppressor. It grows normally in nonsuppressing hosts but does not lyse such strains. The mutation complements with amber mutations in each of the three known phage cistrons. These observations l...

journal_title：Cell

authors： Model P,Webster RE,Zinder ND

更新日期：1979-10-01 00:00:00

abstract：:Constitutive cell-autonomous immunity in metazoans predates interferon-inducible immunity and comprises primordial innate defense. Phagocytes mobilize interferon-inducible responses upon engagement of well-characterized signaling pathways by pathogen-associated molecular patterns (PAMPs). The signals controlling deplo...

journal_title：Cell

authors： Moretti J,Roy S,Bozec D,Martinez J,Chapman JR,Ueberheide B,Lamming DW,Chen ZJ,Horng T,Yeretssian G,Green DR,Blander JM

更新日期：2017-11-02 00:00:00

abstract：:ATP increases microtubule steady state assembly and disassembly rates in vitro in a concentration-dependent manner. Bovine brain microtubules, composed of 75% tubulin and 25% high molecular weight microtubule-associated proteins (MAPs), were purified by three cycles of assembly and disassembly in the absence of ATP. W...

journal_title：Cell

authors： Margolis RL,Wilson L

更新日期：1979-11-01 00:00:00

abstract：:The dentate gyrus of the mammalian hippocampus continuously generates new neurons during adulthood. These adult-born neurons become functionally active and are thought to contribute to learning and memory, especially during their maturation phase, when they have extraordinary plasticity. In this Review, we discuss the...

journal_title：Cell

authors： Gonçalves JT,Schafer ST,Gage FH

更新日期：2016-11-03 00:00:00

abstract：:Metagenomics seeks to characterize the composition of microbial communities, their operations, and their dynamically coevolving relationships with the habitats they occupy without having to culture community members. Uniting metagenomics with analyses of the products of microbial community metabolism (metabolomics) wi...

journal_title：Cell

authors： Turnbaugh PJ,Gordon JI

更新日期：2008-09-05 00:00:00

abstract：:The Wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (Cdk1), thereby delaying entry into mitosis until appropriate conditions have been met. An understanding of the mechanisms that regulate Wee1 should provide new insight into how cells make the decision to enter mitosis. We report here that Swe1, the...

journal_title：Cell

authors： Harvey SL,Charlet A,Haas W,Gygi SP,Kellogg DR

更新日期：2005-08-12 00:00:00

abstract：:The MYC oncoproteins are thought to stimulate tumor cell growth and proliferation through amplification of gene transcription, a mechanism that has thwarted most efforts to inhibit MYC function as potential cancer therapy. Using a covalent inhibitor of cyclin-dependent kinase 7 (CDK7) to disrupt the transcription of a...

journal_title：Cell

authors： Chipumuro E,Marco E,Christensen CL,Kwiatkowski N,Zhang T,Hatheway CM,Abraham BJ,Sharma B,Yeung C,Altabef A,Perez-Atayde A,Wong KK,Yuan GC,Gray NS,Young RA,George RE

更新日期：2014-11-20 00:00:00

abstract：:To determine the extent and nature of genetic variation present in independent isolates of HTLV-III/LAV, the nucleotide sequences of the entire envelope gene and parts of gag and pol were determined for two AIDS viruses. The results indicated that variation throughout the viral genome is extensive and that the envelop...

journal_title：Cell

authors： Starcich BR,Hahn BH,Shaw GM,McNeely PD,Modrow S,Wolf H,Parks ES,Parks WP,Josephs SF,Gallo RC

更新日期：1986-06-06 00:00:00

abstract：:The mammalian alpha- and beta--globin genes are thought to have evolved from a common ancestral sequence by a duplication event that occurred over 500 million years ago. We have now determined the entire nucleotide sequence of a cloned mouse alpha--globin gene, including regions that flank and interrupt the coding seq...

journal_title：Cell

authors： Nishioka Y,Leder P

更新日期：1979-11-01 00:00:00

abstract：:Six restriction fragments of Ad2 DNA which contain sites for RNA initiation have been identified by their ability to hybridize nascent labeled RNA less than 1 kb in length. Four RNA initiation sites early in infection are identified in regions where previous work (Flint, 1977) had mapped mRNAs. The major late RNA init...

journal_title：Cell

authors： Evans RM,Fraser N,Ziff E,Weber J,Wilson M,Darnell JE

更新日期：1977-11-01 00:00:00

abstract：:The angiotensin II (AngII) type 1 receptor (AT1R) is a critical regulator of cardiovascular and renal function and is an important model for studies of G-protein-coupled receptor (GPCR) signaling. By stabilizing the receptor with a single-domain antibody fragment ("nanobody") discovered using a synthetic yeast-display...

journal_title：Cell

authors： Wingler LM,McMahon C,Staus DP,Lefkowitz RJ,Kruse AC

更新日期：2019-01-24 00:00:00

abstract：:The Bcl-2 family proteins are key regulators of apoptosis in human diseases and cancers. Though known to block apoptosis, Bcl-2 promotes cell death through an undefined mechanism. Here, we show that Bcl-2 interacts with orphan nuclear receptor Nur77 (also known as TR3), which is required for cancer cell apoptosis indu...

journal_title：Cell

authors： Lin B,Kolluri SK,Lin F,Liu W,Han YH,Cao X,Dawson MI,Reed JC,Zhang XK

更新日期：2004-02-20 00:00:00

abstract：:The v-erbB gene product of avian erythroblastosis virus (AEV) has extensive homology with the receptor for epidermal growth factor (EGF). We report here that chicken embryo fibroblasts (CEF) transformed by AEV show enhanced tyrosine phosphorylation of a number of cellular polypeptides, including the 36 kd protein, whi...

journal_title：Cell

authors： Gilmore T,DeClue JE,Martin GS

更新日期：1985-03-01 00:00:00