Abstract:
:The extracellular space (ECS) of the brain has an extremely complex spatial organization, which has defied conventional light microscopy. Consequently, despite a marked interest in the physiological roles of brain ECS, its structure and dynamics remain largely inaccessible for experimenters. We combined 3D-STED microscopy and fluorescent labeling of the extracellular fluid to develop super-resolution shadow imaging (SUSHI) of brain ECS in living organotypic brain slices. SUSHI enables quantitative analysis of ECS structure and reveals dynamics on multiple scales in response to a variety of physiological stimuli. Because SUSHI produces sharp negative images of all cellular structures, it also enables unbiased imaging of unlabeled brain cells with respect to their anatomical context. Moreover, the extracellular labeling strategy greatly alleviates problems of photobleaching and phototoxicity associated with traditional imaging approaches. As a straightforward variant of STED microscopy, SUSHI provides unprecedented access to the structure and dynamics of live brain ECS and neuropil.
journal_name
Celljournal_title
Cellauthors
Tønnesen J,Inavalli VVGK,Nägerl UVdoi
10.1016/j.cell.2018.02.007subject
Has Abstractpub_date
2018-02-22 00:00:00pages
1108-1121.e15issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(18)30151-Xjournal_volume
172pub_type
杂志文章相关文献
CELL文献大全abstract::We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90295-7
更新日期:1983-04-01 00:00:00
abstract::CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or parti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.09.014
更新日期:2014-10-09 00:00:00
abstract::Cell lines from rhabdomyosarcomas, which are tumors of muscle origin, have been used as models of CD4-independent HIV infection. These cell lines can be induced to differentiate in vitro. We report here that the vpr gene of HIV1 is sufficient for the differentiation of the human rhabdomyosarcoma cell line TE671. Diffe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90073-y
更新日期:1993-02-26 00:00:00
abstract::AU-rich elements (AREs), present in mRNA 3'-UTRs, are potent posttranscriptional regulatory signals that can rapidly effect changes in mRNA stability and translation, thereby dramatically altering gene expression with clinical and developmental consequences. In human cell lines, the TNFalpha ARE enhances translation r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.01.038
更新日期:2007-03-23 00:00:00
abstract::Vg1 is a TGF beta-related growth factor encoded by a maternal mRNA localized to vegetal blastomeres in Xenopus embryos. Vg1 precursor protein is abundant in vegetal cells, but the processed mature form has not been readily detected and no activity has been demonstrated for the putative Vg1 mature protein. We have engi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)80045-g
更新日期:1993-08-13 00:00:00
abstract::In RNA-mediated interference (RNAi), double-stranded RNAs (dsRNAs) target complementary mRNAs for degradation. New work demonstrates that essential chromosomal functions are mediated through RNAi protein components and short RNAs, which alter chromosome function at specific DNA loci via histone modification. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01039-5
更新日期:2002-10-18 00:00:00
abstract::SOCS1 is an SH2-containing protein that is primarily expressed in thymocytes in a cytokine- and T cell receptor-independent manner. SOCS1 deletion causes perinatal lethality with death by 2-3 weeks. During this period thymic changes include a loss of cellularity and a switch from predominantly CD4+ CD8+ to single posi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80048-3
更新日期:1999-09-03 00:00:00
abstract::To understand the role of FoxO family members in hematopoiesis, we conditionally deleted FoxO1, FoxO3, and FoxO4 in the adult hematopoietic system. FoxO-deficient mice exhibited myeloid lineage expansion, lymphoid developmental abnormalities, and a marked decrease of the lineage-negative Sca-1+, c-Kit+ (LSK) compartme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.01.003
更新日期:2007-01-26 00:00:00
abstract::Spotted fever group (SFG) rickettsiae are human pathogens that infect cells in the vasculature. They disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. Other bacterial pathogens rely on actin-based motility to provide a physical for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.09.023
更新日期:2016-10-20 00:00:00
abstract::It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 phys...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.06.028
更新日期:2014-08-14 00:00:00
abstract::There are still no effective treatments to prevent, halt, or reverse Alzheimer's disease, but research advances over the past three decades could change this gloomy picture. Genetic studies demonstrate that the disease has multiple causes. Interdisciplinary approaches combining biochemistry, molecular and cell biology...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2012.02.040
更新日期:2012-03-16 00:00:00
abstract::Mosquito-borne flaviviruses, including dengue virus (DENV) and Zika virus (ZIKV), are a growing public health concern. Systems-level analysis of how flaviviruses hijack cellular processes through virus-host protein-protein interactions (PPIs) provides information about their replication and pathogenic mechanisms. We u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.028
更新日期:2018-12-13 00:00:00
abstract::We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90028-3
更新日期:1981-10-01 00:00:00
abstract::Ubiquitin-conjugating enzyme variant (UEV) proteins resemble ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue. The MMS2-encoded UEV protein has been genetically implicated in error-free postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms a specific heteromer...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80575-9
更新日期:1999-03-05 00:00:00
abstract::Several metabolic alterations accumulate over time along with a reduction in biological fitness, suggesting the existence of a "metabolic clock" that controls aging. Multiple inborn defects in metabolic circuitries accelerate aging, whereas genetic loci linked to exceptional longevity influence metabolism. Each of the...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.07.031
更新日期:2016-08-11 00:00:00
abstract::Hsp33, a member of a newly discovered heat shock protein family, was found to be a very potent molecular chaperone. Hsp33 is distinguished from all other known molecular chaperones by its mode of functional regulation. Its activity is redox regulated. Hsp33 is a cytoplasmically localized protein with highly reactive c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80547-4
更新日期:1999-02-05 00:00:00
abstract::During metastasis, migrating breast cancer stem cells undergo a loss of polarity leading to an epithelial-to-mesenchymal transition (EMT). Gupta et al. (2009) use this attribute of cancer stem cells to develop a high-throughput screen, which successfully identifies small molecules that specifically inhibit cancer stem...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2009.08.007
更新日期:2009-08-21 00:00:00
abstract::Social isolation is a stressful condition that often leads to maladaptive behaviors. In this issue of Cell, Zelikowsky et al. find that chronic social isolation stress triggers an increase in neuronal tachykinin signaling across distinct brain regions that mediate fear and aggression, elucidating the neural basis of t...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2018.04.031
更新日期:2018-05-17 00:00:00
abstract::Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90568-5
更新日期:1988-05-20 00:00:00
abstract::The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.10.032
更新日期:2010-11-12 00:00:00
abstract::Inositol phosphates are well-known signaling molecules, whereas the inositol pyrophosphates, such as diphosphoinositol pentakisphosphate (InsP7/IP7) and bis-diphosphoinositol tetrakisphosphate (InsP8/IP8), are less well characterized. We demonstrate physiologic regulation of Dictyostelium chemotaxis by InsP7 mediated ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00640-8
更新日期:2003-09-05 00:00:00
abstract::Based on the above mechanisms of mRNA degradation, an integrated model of mRNA turnover can be proposed (Figure 1). In this model, all polyadenylated mRNAs would be degraded by the deadenylation-dependent pathway at some rate. In addition to this default pathway, another layer of complexity would come from degradation...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/0092-8674(95)90326-7
更新日期:1995-04-21 00:00:00
abstract::Induction of senescence permanently restricts cellular proliferation after oncogenic stimulation thereby acting as a potent barrier to tumor development. The relevant effector proteins may therefore be fundamental to cancer development. A recent study identified IGFBP7 as a secreted factor mediating melanocyte senesce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.04.021
更新日期:2010-05-14 00:00:00
abstract::T lymphocyte selection and lineage commitment in the thymus requires multiple signals. Herein, CD4+ T cell generation required engagement of CD83, a surface molecule expressed by thymic epithelial and dendritic cells. CD83-deficient (CD83-/-) mice had a specific block in CD4+ single-positive thymocyte development with...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00673-6
更新日期:2002-03-22 00:00:00
abstract::Polycomb group (PcG) proteins form large multimeric complexes (PcG bodies) which are involved in the stable repression of gene expression. The human PcG protein, Pc2, has been shown to recruit the transcriptional corepressor, CtBP, to PcG bodies. We show that CtBP is sumoylated at a single lysine. In vitro, CtBP sumoy...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00159-4
更新日期:2003-04-04 00:00:00
abstract::Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90248-3
更新日期:1982-09-01 00:00:00
abstract::Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.06.006
更新日期:2011-07-08 00:00:00
abstract::Uracil-DNA glycosylase inhibitor (Ugi) is a B. subtilis bacteriophage protein that protects the uracil-containing phage DNA by irreversibly inhibiting the key DNA repair enzyme uracil-DNA glycosylase (UDG). The 1.9 A crystal structure of Ugi complexed to human UDG reveals that the Ugi structure, consisting of a twiste...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90467-0
更新日期:1995-09-08 00:00:00
abstract::Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.024
更新日期:2017-02-23 00:00:00
abstract::The scrapie (PrPSc) and cellular (PrPC) prion proteins are encoded by the same gene, and their different properties are thought to arise from posttranslational modifications. We have found a phosphatidylinositol glycolipid on both PrPC and PrP 27-30 (derived from PrPSc by limited proteolysis at the amino terminus). Et...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90150-4
更新日期:1987-10-23 00:00:00