Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: protein mimicry of DNA.

abstract：:Genetic and transcriptional maps have been constructed for the mitochondrial genome of the Ascomycete Podospora anserina. These data have been plotted on the restriction maps for Sal I, Xho I, Bam HI, Eco RI, BgI II and Hae III. We have characterized and cloned a new and unique senescent mitochondrial DNA (beta-event ...

journal_title：Cell

authors： Wright RM,Horrum MA,Cummings DJ

更新日期：1982-06-01 00:00:00

abstract：:ATP-dependent proteases are vital to maintain cellular protein homeostasis. Here, we study the mechanisms of force generation and intersubunit coordination in the ClpXP protease from E. coli to understand how these machines couple ATP hydrolysis to mechanical protein unfolding. Single-molecule analyses reveal that pho...

journal_title：Cell

authors： Sen M,Maillard RA,Nyquist K,Rodriguez-Aliaga P,Pressé S,Martin A,Bustamante C

更新日期：2013-10-24 00:00:00

abstract：:Pejvakin (PJVK), a protein originally identified in Persian families with sensorineural hearing loss, regulates peroxisomal dynamics and the antioxidant defense triggered by noise exposure in hair cells and auditory neurons of the inner ear. These findings bring peroxisomes to the forefront of noise-induced hearing lo...

journal_title：Cell

authors： Mardones P,Hetz C

更新日期：2015-11-05 00:00:00

abstract：:Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted P...

journal_title：Cell

authors： Woodruff JB,Ferreira Gomes B,Widlund PO,Mahamid J,Honigmann A,Hyman AA

更新日期：2017-06-01 00:00:00

abstract：:Prolonged behavioral challenges can cause animals to switch from active to passive coping strategies to manage effort-expenditure during stress; such normally adaptive behavioral state transitions can become maladaptive in psychiatric disorders such as depression. The underlying neuronal dynamics and brainwide interac...

journal_title：Cell

authors： Andalman AS,Burns VM,Lovett-Barron M,Broxton M,Poole B,Yang SJ,Grosenick L,Lerner TN,Chen R,Benster T,Mourrain P,Levoy M,Rajan K,Deisseroth K

更新日期：2019-05-02 00:00:00

abstract：:S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...

journal_title：Cell

authors： Mayer A,Wickner W,Haas A

更新日期：1996-04-05 00:00:00

abstract：:The tyrT locus of E. coli contains a 208 bp spacer region that separates two copies of sequence encoding tRNATyr1. The spacer includes a 120 bp sequence that is homologous to a sequence that is repeated three times in the distal portion of the tyrT locus. The tyrT locus possesses a graded set of transcription terminat...

journal_title：Cell

authors： Rossi J,Egan J,Hudson L,Landy A

更新日期：1981-11-01 00:00:00

abstract：:Through dissecting the link between spatial genome organization and DNA replication timing, Sima et al. (2018) discover early replicating control elements (ERCEs), a new type of cis-acting elements that regulate replication timing, transcription, and multiple layers of three-dimensional features of genome organization...

journal_title：Cell

authors： Ma J,Duan Z

更新日期：2019-02-07 00:00:00

abstract：:Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...

journal_title：Cell

authors： Méthot N,Basler K

更新日期：1999-03-19 00:00:00

abstract：:The expression of the E2A transcription unit of the adenovirus E1A deletion mutant dl312 in murine embryonal carcinoma (EC) stem cells suggests that they contain an activity that will complement viral E1A. We have prepared from these cells in vitro transcription extracts that use E1A-inducible promoters more efficient...

journal_title：Cell

authors： La Thangue NB,Rigby PW

更新日期：1987-05-22 00:00:00

abstract：:We have shown that in fixed mitotic chromosomes from female G. gerbillus cells the inactive X chromosome is distinctly less sensitive to DNAase I than the active X chromosome, as demonstrated by in situ nick translation. These results indicated that the specific chromatin conformation that renders potentially active g...

journal_title：Cell

authors： Kerem BS,Goitein R,Diamond G,Cedar H,Marcus M

更新日期：1984-09-01 00:00:00

abstract：:Junctions that connect the endoplasmic reticulum (ER) and the plasma membrane (PM) are unique yet ubiquitous subcellular compartments. Giordano et al. now report that extended synaptotagmins (E-Syts) promote their formation, providing fundamental insight into the molecular machinery controlling ER and plasma membrane ...

journal_title：Cell

authors： Malmersjö S,Meyer T

更新日期：2013-06-20 00:00:00

abstract：:DDB1, a component of the Cul4 ubiquitin ligase complex, promotes protein ubiquitination in diverse cellular functions, including nuclear excision repair, regulation of the cell cycle, and DNA replication. To investigate its physiological significance, we generated mice with null and floxed alleles of the DDB1 gene. He...

journal_title：Cell

authors： Cang Y,Zhang J,Nicholas SA,Bastien J,Li B,Zhou P,Goff SP

更新日期：2006-12-01 00:00:00

abstract：:In Drosophila, the fat body (FB), a functional analog of the vertebrate adipose tissue, is the nutrient sensor that conveys the nutrient status to the insulin-producing cells (IPCs) in the fly brain to release Drosophila insulin-like peptides (Dilps). Dilp secretion in turn regulates energy balance and promotes system...

journal_title：Cell

authors： Rajan A,Perrimon N

更新日期：2012-09-28 00:00:00

abstract：:We have analyzed the generation of unexpected patterns of RNA editing, i.e., those not following a strict 3' to 5' progression, which occur in junction regions between fully edited and preedited sequences. Evidence is presented that these patterns are generated by misediting due to specific events of misguiding. Mised...

journal_title：Cell

authors： Sturm NR,Maslov DA,Blum B,Simpson L

更新日期：1992-08-07 00:00:00

abstract：:gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...

journal_title：Cell

authors： Dulić V,Kaufmann WK,Wilson SJ,Tlsty TD,Lees E,Harper JW,Elledge SJ,Reed SI

更新日期：1994-03-25 00:00:00

abstract：:Insulin receptor (IR) signaling is central to normal metabolic control and dysregulated in prevalent chronic diseases. IR binds insulin at the cell surface and transduces rapid signaling via cytoplasmic kinases. However, mechanisms mediating long-term effects of insulin remain unclear. Here, we show that IR associates...

journal_title：Cell

authors： Hancock ML,Meyer RC,Mistry M,Khetani RS,Wagschal A,Shin T,Ho Sui SJ,Näär AM,Flanagan JG

更新日期：2019-04-18 00:00:00

abstract：:A complex interplay of environmental factors impacts the metabolism of human cells, but neither traditional culture media nor mouse plasma mimic the metabolite composition of human plasma. Here, we developed a culture medium with polar metabolite concentrations comparable to those of human plasma (human plasma-like me...

journal_title：Cell

authors： Cantor JR,Abu-Remaileh M,Kanarek N,Freinkman E,Gao X,Louissaint A Jr,Lewis CA,Sabatini DM

更新日期：2017-04-06 00:00:00

abstract：:Rho family GTPases are thought to regulate actin-dependent processes, but their functions in vivo are still poorly understood. We have investigated the function of a new, widely expressed Rho family member in C. elegans by analyzing mutations in the endogenous gene. Activated and null alleles all inhibit cell migratio...

journal_title：Cell

authors： Zipkin ID,Kindt RM,Kenyon CJ

更新日期：1997-09-05 00:00:00

abstract：:To study the insertion of multispanning membrane proteins into the endoplasmic reticulum, we constructed novel proteins on the cDNA level by repeating, up to four times, the internal signal-anchor domain of the asialoglycoprotein receptor H1. Upon in vitro translation in the presence of microsomes, these polypeptides ...

journal_title：Cell

authors： Wessels HP,Spiess M

更新日期：1988-10-07 00:00:00

abstract：:At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...

journal_title：Cell

authors： Zakharyevich K,Tang S,Ma Y,Hunter N

更新日期：2012-04-13 00:00:00

abstract：:Retroviral DNA integration involves a coordinated set of DNA cutting and joining reactions. We find that the IN protein of Moloney murine leukemia virus (MoMLV) is the only viral protein required to accomplish these reactions in vitro. IN protein has a site-specific nuclease activity that cleaves 2 nucleotides from th...

journal_title：Cell

authors： Craigie R,Fujiwara T,Bushman F

更新日期：1990-08-24 00:00:00

abstract：:The synthesis and secretion of plasminogen activator by cultured macrophages can be induced and stimulated by concanavalin A and by phorbol myristate acetate, and inhibited by such agents as glucocorticoids, mitotic inhibitors and compounds affecting cAMP metabolism. By the manipulation of stimulatory and inhibitory i...

journal_title：Cell

authors： Vassalli JD,Hamilton J,Reich E

更新日期：1977-07-01 00:00:00

abstract：:Circadian rhythms control organismal physiology throughout the day. At the cellular level, clock regulation is established by a self-sustained Bmal1-dependent transcriptional oscillator network. However, it is still unclear how different tissues achieve a synchronized rhythmic physiology. That is, do they respond inde...

journal_title：Cell

authors： Welz PS,Zinna VM,Symeonidi A,Koronowski KB,Kinouchi K,Smith JG,Guillén IM,Castellanos A,Furrow S,Aragón F,Crainiciuc G,Prats N,Caballero JM,Hidalgo A,Sassone-Corsi P,Benitah SA

更新日期：2019-05-30 00:00:00

abstract：:The BALC/c mouse has two nonallelic beta-globin genes that appear to reside on two different Eco R1 fragments of genomic DNA. We have already cloned one of these fragments and shown that the gene encoded within it is interrupted by at least one large intervening sequence of DNA. We have now cloned and characterized th...

journal_title：Cell

authors： Tiemeier DC,Tilghman SM,Polsky FI,Seidman JG,Leder A,Edgell MH,Leder P

更新日期：1978-06-01 00:00:00

abstract：:Protein-DNA binding is mediated by the recognition of the chemical signatures of the DNA bases and the 3D shape of the DNA molecule. Because DNA shape is a consequence of sequence, it is difficult to dissociate these modes of recognition. Here, we tease them apart in the context of Hox-DNA binding by mutating residues...

journal_title：Cell

authors： Abe N,Dror I,Yang L,Slattery M,Zhou T,Bussemaker HJ,Rohs R,Mann RS

更新日期：2015-04-09 00:00:00

abstract：:The structure and organization on the Escherichia coli chromosome of the gene cluster coding for two methionine tRNAs (tRNAmMet), four glutamine tRNAs (two tRNA1Gln and two tRNA2Gln), and a previously unidentified tRNA (called tRNAx) have been studied by restriction enzyme analysis and DNA sequencing, utilizing a spec...

journal_title：Cell

authors： Nakajima N,Ozeki H,Shimura Y

更新日期：1981-01-01 00:00:00

abstract：:ATRX is an X-linked gene of the SWI/SNF family, mutations in which cause syndromal mental retardation and downregulation of α-globin expression. Here we show that ATRX binds to tandem repeat (TR) sequences in both telomeres and euchromatin. Genes associated with these TRs can be dysregulated when ATRX is mutated, and ...

journal_title：Cell

authors： Law MJ,Lower KM,Voon HP,Hughes JR,Garrick D,Viprakasit V,Mitson M,De Gobbi M,Marra M,Morris A,Abbott A,Wilder SP,Taylor S,Santos GM,Cross J,Ayyub H,Jones S,Ragoussis J,Rhodes D,Dunham I,Higgs DR,Gibbons RJ

更新日期：2010-10-29 00:00:00

abstract：:We have compared the chromatin structure in the active and inactive states at loci encoding the major heat shock protein in Drosophila. DNAase I and micrococcal nuclease were used as probes of higher order organization and nucleosomal integrity. Such integrity is gauged here by the characteristic pattern of discrete D...

journal_title：Cell

authors： Wu C,Wong YC,Elgin SC

更新日期：1979-04-01 00:00:00

abstract：:SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycle of preprotein translocation. When SecA is complexed with SecY/E and preprotein, ATP drives a profound conformational change, leading to membrane insertion of a 30...

journal_title：Cell

authors： Economou A,Wickner W

更新日期：1994-09-09 00:00:00