Abstract:
:The susceptibility to repair of particular base mismatches by the hex system of Streptococcus pneumoniae was examined by comparison of the nucleotide sequence of the wild-type and eight mutant alleles of the malM gene. A detailed restriction map was constructed for pLS70, and the nucleotide sequence was determined for its 3475 bp chromosomal insert, which contains the entire malM gene (encoding amylomaltase), portions of malX and malP (encoding a membrane protein and a phosphorylase, respectively) and a control region. Transition mismatches were highly susceptible to repair; transversion mismatches, much less so. A mismatch caused by a single-nucleotide deletion was reparable, but mismatches with longer deletions were not. The hex system also reduced spontaneous reversion of mutations corresponding to transitions. It is suggested that recognition of donor or nascent DNA strands by the hex system depends on single-strand breaks in the target strand, and that the role of DNA methylation in mismatch repair of Escherichia coli can be accommodated to this model.
journal_name
Celljournal_title
Cellauthors
Lacks SA,Dunn JJ,Greenberg Bdoi
10.1016/0092-8674(82)90126-xsubject
Has Abstractpub_date
1982-12-01 00:00:00pages
327-36issue
2 Pt 1eissn
0092-8674issn
1097-4172pii
0092-8674(82)90126-Xjournal_volume
31pub_type
杂志文章相关文献
CELL文献大全abstract::The minimal DNA sequence required for the formation of a stable complex with Drosophila heat shock factor (HSF) in vitro is an inverted repeat of a 5 bp recognition unit, -GAA-. Surprisingly, both permutations of this 5 bp unit, head-to-head and tail-to-tail, bind to HSF with similar affinity and with striking 2-fold ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90603-x
更新日期:1989-12-01 00:00:00
abstract::The adenovirus E1a region encodes two closely related gene products: 243 and 289 amino acid phosphoproteins. These proteins differ in their primary sequence only by 46 amino acids unique to the 289 amino acid protein. By constructing single-base substitution mutants we localized two functional regions of these E1a pro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90704-x
更新日期:1986-09-26 00:00:00
abstract::The actions and receptor for ciliary neurotrophic factor (CNTF) are largely restricted to cells of the nervous system, although one of the CNTF receptor components, CNTFR alpha, is expressed by skeletal muscle. Here we show that the other CNTF receptor components, LIFR beta and gp130, are also expressed by skeletal mu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90113-9
更新日期:1994-02-11 00:00:00
abstract::Activation of Agouti-related peptide (AgRP) neurons potently promotes feeding, and chronically altering their activity also affects peripheral glucose homeostasis. We demonstrate that acute activation of AgRP neurons causes insulin resistance through impairment of insulin-stimulated glucose uptake into brown adipose t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.02.044
更新日期:2016-03-24 00:00:00
abstract::The origin of a low molecular weight virus-associated RNA (VA-RNA) was mapped by hybridization of VA-RNA to specific fragments of adenovirus type 2 DNA, obtained after cleavage with three different restriction endonucleases. VA-RNA was found to hybridize exculsively to the l-strand [strand with low buoyant density in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90066-5
更新日期:1975-09-01 00:00:00
abstract::Increased protein synthesis plays an etiologic role in diverse cancers. Here, we demonstrate that METTL13 (methyltransferase-like 13) dimethylation of eEF1A (eukaryotic elongation factor 1A) lysine 55 (eEF1AK55me2) is utilized by Ras-driven cancers to increase translational output and promote tumorigenesis in vivo. ME...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.11.038
更新日期:2019-01-24 00:00:00
abstract::Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae. Here, we present evidence that Ste5 forms a multikinase complex that joins these kinases for efficient Fus3 activation. B...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90427-8
更新日期:1994-08-12 00:00:00
abstract::We have isolated five clones containing human histone genes from a human genomic DNA library, using a cloned X. laevis histone H4 cDNA probe (pX1ch4). Each genomic clone has been mapped and the locations of the histone genes have been determined by blot hybridization and hybridization-selection of human histone mRNA. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90092-1
更新日期:1981-06-01 00:00:00
abstract::The topology of E. coli inner membrane proteins depends primarily on the distribution of positively charged residues in the molecule. We have constructed model proteins with four potential transmembrane stretches and have systematically explored the topological effects of lysines placed in the loops connecting the tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90155-4
更新日期:1994-05-06 00:00:00
abstract::In vitro enzymatic alteration of plasmid phenotype and in vitro construction of recombinant plasmids containing genetic information derived from the plasmid pSC101 have been used to investigate the mechanism of function of tetracycline resistance determined by the plasmid pSC101. The resistance has been shown to be in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90139-3
更新日期:1978-01-01 00:00:00
abstract::In this issue of Cell, two articles show that tumor-specific changes in HLA-mediated antigen presentation affect tumor immunogenicity and may play a role in shaping cancer cell survival. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.11.012
更新日期:2017-11-30 00:00:00
abstract::The human geneticist Archibald Garrod noted in 1931 that, "It is, of necessity, no easy matter to distinguish between immunity which is inborn and that which has been acquired" (The Inborn Factors in Disease). In this issue of Cell, Brodin et al. show that the heritability of blood counts rapidly decreases with age fo...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.12.029
更新日期:2015-01-15 00:00:00
abstract::The p53 tumor suppressor gene is commonly altered in human tumors, predominantly through missense mutations that result in accumulation of mutant p53 protein. These mutations may confer dominant-negative or gain-of-function properties to p53. To ascertain the physiological effects of p53 point mutation, the structural...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.004
更新日期:2004-12-17 00:00:00
abstract::To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90494-0
更新日期:1986-01-17 00:00:00
abstract::Misfolded proteins accumulating in several neurodegenerative diseases (including Alzheimer, Parkinson, and Huntington diseases) can cause aggregation of their native counterparts through a mechanism similar to the infectious prion protein's induction of a pathogenic conformation onto its cellular isoform. Evidence for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.011
更新日期:2011-10-28 00:00:00
abstract::The JAK/STAT signaling pathway, renowned for its effects on cell proliferation and survival, is constitutively active in various human cancers, including ovarian. We have found that JAK and STAT are required to convert the border cells in the Drosophila ovary from stationary, epithelial cells to migratory, invasive ce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00607-9
更新日期:2001-12-28 00:00:00
abstract::Bacteria are known to communicate primarily via secreted extracellular factors. Here we identify a previously uncharacterized type of bacterial communication mediated by nanotubes that bridge neighboring cells. Using Bacillus subtilis as a model organism, we visualized transfer of cytoplasmic fluorescent molecules bet...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.01.015
更新日期:2011-02-18 00:00:00
abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.063
更新日期:2012-02-17 00:00:00
abstract::Sir2 is a conserved deacetylase that modulates life span in yeast, worms, and flies and stress response in mammals. In yeast, Sir2 is required for maintaining replicative life span, and increasing Sir2 dosage can delay replicative aging. We address the role of Sir2 in regulating chronological life span in yeast. Lack ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.08.042
更新日期:2005-11-18 00:00:00
abstract::The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.09.053
更新日期:2015-10-22 00:00:00
abstract::The specific patterns and functional properties of electrical synapses of a nervous system are defined by the neuron-specific complement of electrical synapse constituents. We systematically examined the molecular composition of the electrical connectome of the nematode C. elegans through a genome- and nervous-system-...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.12.024
更新日期:2019-02-21 00:00:00
abstract::Xenopus laevis oocytes were used as a model system to study the intracellular fate of proteins incorporated by endocytosis. We found that the intracellular stability and compartmentation of proteins incorporated by receptor-mediated endocytosis differed substantially from that of proteins incorporated by nonspecific e...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90153-1
更新日期:1980-11-01 00:00:00
abstract::Mutually exclusive expression within the var gene family of the malaria parasite Plasmodium falciparum is important for parasite survival and virulence. In this issue of Cell, Duraisingh et al. and Freitas-Junior et al. provide evidence for the role of Sir2-dependent alterations in chromatin structure and changes in s...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2005.03.019
更新日期:2005-04-08 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) binds to cells through interactions of viral glycoproteins gB and gC with heparan sulfate chains on cell surface proteoglycans. This binding is not sufficient for viral entry, which requires fusion between the viral envelope and cell membrane. Here, we show that heparan sulfate modi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80058-6
更新日期:1999-10-01 00:00:00
abstract::Ras-related GTPases are positively regulated by guanine nucleotide exchange factors (GEFs) that promote the exchange of GDP for GTP. The crystal structure of the Sec7 domain GEF bound to nucleotide-free ARF1 GTPase has been determined at 2.8 A resolution and the structure of ARF1 in the GTP-analog form determined at 1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81754-7
更新日期:1998-10-16 00:00:00
abstract::Structures of 70 unique G protein-coupled receptors (GPCRs) have been determined, with over 370 structures in total bound to different ligands and the receptors in various conformational states. Structure-based drug design has been applied to an increasing number of GPCR targets over the past decade and now a few of t...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2020.03.003
更新日期:2020-04-02 00:00:00
abstract::The pluripotential hemopoietic stem cells (CFU-S) of genetically resistant Fv2rr adult C57BL/6 (B6) mice can be persistently infected by Friend virus (FV) when exposed to defective spleen focus-forming virus (SFFV) and helper virus (LLV) at the time of transplantation into heavily irradiated syngeneic recipients (that...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90327-0
更新日期:1982-12-01 00:00:00
abstract::3.18 kb fragments of X. laevis DNA coding for tRNA 1 met have been inserted into a lambda vector via Hind III termini and cloned in E. coli. The organization of one cloned fragment has been analyzed by restriction endonuclease digestion and RNA-DNA hybridization. From the distribution of sites for three enzymes, this ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90253-2
更新日期:1978-07-01 00:00:00
abstract::The rate-limiting enzyme of cholesterol biosynthesis, HMG CoA reductase, is controlled by negative feedback regulation of transcription. We have isolated the reductase gene from a bacteriophage lambda genomic library prepared from hamster UT-1 cells. The 25 kilobase gene is split into 20 exons. The 5' untranslated and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90549-x
更新日期:1984-08-01 00:00:00
abstract::We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00700-6
更新日期:2002-04-19 00:00:00