Abstract:
:Transcription termination by bacterial RNA polymerase (RNAP) occurs at sequences coding for a GC-rich RNA hairpin followed by a U-rich tract. We used single-molecule techniques to investigate the mechanism by which three representative terminators (his, t500, and tR2) destabilize the elongation complex (EC). For his and tR2 terminators, loads exerted to bias translocation did not affect termination efficiency (TE). However, the force-dependent kinetics of release and the force-dependent TE of a mutant imply a forward translocation mechanism for the t500 terminator. Tension on isolated U-tracts induced transcript release in a manner consistent with RNA:DNA hybrid shearing. We deduce that different mechanisms, involving hypertranslocation or shearing, operate at terminators with different U-tracts. Tension applied to RNA at terminators suggests that closure of the final 2-3 hairpin bases destabilizes the hybrid and that competing RNA structures modulate TE. We propose a quantitative, energetic model that predicts the behavior for these terminators and mutant variants.
journal_name
Celljournal_title
Cellauthors
Larson MH,Greenleaf WJ,Landick R,Block SMdoi
10.1016/j.cell.2008.01.027subject
Has Abstractpub_date
2008-03-21 00:00:00pages
971-82issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(08)00127-Xjournal_volume
132pub_type
杂志文章相关文献
CELL文献大全abstract::The brlA gene of A. nidulans mediates the developmental switch from the indeterminate, apical growth pattern of vegetative cells to the budding growth pattern of conidiophores. brlA encodes a 432 amino acid polypeptide containing two directly repeated motifs resembling the Zn(II) coordination sites first recognized in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90198-5
更新日期:1988-07-29 00:00:00
abstract::Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.07.030
更新日期:2005-10-07 00:00:00
abstract::In induced erythroleukemia cells, transcription of the beta-globin gene terminates in a region 600-1500 nucleotides downstream of the poly(A) site. To determine whether this region of the mouse DNA functions to terminate transcription when moved to another genomic site, portions of the putative termination region have...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90349-6
更新日期:1985-04-01 00:00:00
abstract::We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells wit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90035-6
更新日期:1982-08-01 00:00:00
abstract::p11, through unknown mechanisms, is required for behavioral and cellular responses to selective serotonin reuptake inhibitors (SSRIs). We show that SMARCA3, a chromatin-remodeling factor, is a target for the p11/annexin A2 heterotetrameric complex. Determination of the crystal structure indicates that SMARCA3 peptide ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.01.014
更新日期:2013-02-14 00:00:00
abstract::Eukaryotic cells sterilize the cytosol by using autophagy to route invading bacterial pathogens to the lysosome. During macrophage infection with Mycobacterium tuberculosis, a vacuolar pathogen, exogenous induction of autophagy can limit replication, but the mechanism of autophagy targeting and its role in natural inf...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.06.040
更新日期:2012-08-17 00:00:00
abstract::RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide R...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.048
更新日期:2014-05-22 00:00:00
abstract::Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.033
更新日期:2009-12-11 00:00:00
abstract::A selection procedure is described that should allow the genetic identification of cellular components involved in the process of protein localization in Escherichia coli. This procedure makes use of mutations that alter the signal sequence of the lambda receptor protein (product of the lamB gene), and prevent export ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90272-5
更新日期:1981-01-01 00:00:00
abstract::DNA transcription, replication, and repair are regulated by histone acetylation, a process that requires the generation of acetyl-coenzyme A (CoA). Here, we show that all the subunits of the mitochondrial pyruvate dehydrogenase complex (PDC) are also present and functional in the nucleus of mammalian cells. We found t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.04.046
更新日期:2014-07-03 00:00:00
abstract::We used the interaction trap, a yeast genetic selection for interacting proteins, to isolate human cyclin-dependent kinase interactor 1 (Cdi1). In yeast, Cdi1 interacts with cyclin-dependent kinases, including human Cdc2, Cdk2, and Cdk3, but not with Ckd4. In HeLa cells, Cdi1 is expressed at the G1 to S transition, an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90498-f
更新日期:1993-11-19 00:00:00
abstract::The alpha-amylase mRNAs which accumulate in two different tissues of the mouse, the salivary gland and the liver, are identical except for their 5' non-translated sequences: the 5' terminal 158 nucleotides of the major liver alpha-amylase mRNA are unrelated to the 5' terminal 47 nucleotides found in its salivary gland...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90140-9
更新日期:1981-02-01 00:00:00
abstract::The erythropoietin receptor (EpoR) is essential for production of red blood cells; a principal function of EpoR is to rescue committed erythroid progenitors from apoptosis. Stat5 is rapidly activated following EpoR stimulation, but its function in erythropoiesis has been unclear since adult Stat5a-/-5b-/- mice have no...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81013-2
更新日期:1999-07-23 00:00:00
abstract::Most human epithelial tumors harbor numerous alterations, making it difficult to predict which genes are required for tumor survival. To systematically identify cancer dependencies, we analyzed 501 genome-scale loss-of-function screens performed in diverse human cancer cell lines. We developed DEMETER, an analytical f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.06.010
更新日期:2017-07-27 00:00:00
abstract::We have used cloned chicken cDNA sequences for alpha- and beta-tubulin to investigate tubulin gene organization in Drosophila melanogaster. Experiments using genomic Drosophila DNA from several sources indicate that there are at least four copies each of the alpha-tubulin gene and the beta-tubulin gene. In situ hybrid...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90561-9
更新日期:1980-12-01 00:00:00
abstract::Polyadenylation of mRNA precursors by poly(A) polymerase depends on a specificity factor, CPF, recognizing the polyadenylation signal AAUAAA. This paper describes an apparently novel poly(A)-binding protein that acts as a second specificity factor, mediating the recognition of the growing poly(A) tail. A transition fr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90119-j
更新日期:1991-08-23 00:00:00
abstract::The organization of neuronal systems is often dependent on activity and competition between cells. In olfaction, the X-linked OCNC1 channel subunit is subject to random inactivation and is essential for odorant-evoked activity. Reporter-tagged OCNC1 mutant mice permit the visualization of OCNC1-deficient olfactory neu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00262-8
更新日期:2001-03-09 00:00:00
abstract::Plasma membrane vesicles and protoplasts of Escherichia coli were fused to planar lipid bilayers and studied with electrophysiological techniques. Large transmembrane aqueous channels were opened when 0.2 nM LamB signal peptide was added to the cytoplasmic side of the membrane. These aqueous pores are similar in condu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90231-z
更新日期:1992-05-15 00:00:00
abstract::Secretory proteins are segregated from cytosolic proteins by their translocation into the endoplasmic reticulum (ER). A modified secretory protein trapped during translocation across the ER membrane can be crosslinked to two previously identified proteins, Sec61p and BiP (Kar2p). The dependence of this cross-linking u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90415-9
更新日期:1992-04-17 00:00:00
abstract::We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics of 139 COVID-19 patients representing all levels of disease severity, from serial blood draws collected during the first week of infection following diagnosis. We identify a major shift between mild and moderate diseas...
journal_title:Cell
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.cell.2020.10.037
更新日期:2020-12-10 00:00:00
abstract::Using a combination of classical genetics, serology, and molecular genetics, we have mapped as many as possible of the MHC components in t haplotypes. Results indicate that the whole MHC is included in a simple inversion with the relative positions of its genes being intact. Furthermore, some of the t-lethal mutations...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90464-1
更新日期:1984-12-01 00:00:00
abstract::An examination of Abelson murine leukemia virus (A-MuL V)-hematopoietic cell interaction in cultures of fetal tissues reveals that A-MuLV can stimulate the formation of two different types of colonies. One type of colony is white and composed of A-MuLV-transformed lymphoid cells that can develop into established cell ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90035-0
更新日期:1981-10-01 00:00:00
abstract::Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions and can augment or diversify existing functions. Here, we report a gene duplication event that yielded another outcome--the generation of antagonistic functions. One product of this dupli...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.02.046
更新日期:2016-04-07 00:00:00
abstract::We wished to learn whether the genomes of strains of EMB isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from Burkitt's lymphoma. The genome of a new transforming strains (FF41) of EBV isolated from saliva of a patient with uncomplicated infectious mononucleo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90345-7
更新日期:1981-05-01 00:00:00
abstract::Steroid hormones induce transcription from the mouse mammary tumor virus (MMTV) promoter by complex mechanisms requiring binding of the hormone receptors to the hormone responsive element (HRE) of the long terminal repeat region. Here we show that the MMTV promoter contains two degenerated octamer motifs immediately u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90240-y
更新日期:1991-02-08 00:00:00
abstract::Beta-arrestin1 is a multifunctional protein critically involved in signal transduction. Recently, it is also identified as a nuclear transcriptional regulator, but the underlying mechanisms and physiological significance remain to be explored. Here, we identified beta-arrestin1 as an evolutionarily conserved protein e...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.038
更新日期:2009-10-30 00:00:00
abstract::The eukaryotic 20S proteasome is known to associate with the IFN gamma-inducible regulator PA28. We analyzed the kinetics of product generation by 20S proteasomes with and without PA28. In the absence of PA28, the 20S proteasome rapidly generates peptides that have been cleaved only once, while internal fragments accu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80097-5
更新日期:1996-07-26 00:00:00
abstract::Transplantation of pronuclei between one-cell-stage embryos was used to construct diploid mouse embryos with two female pronuclei ( biparental gynogenones ) or two male pronuclei ( biparental androgenones ). The ability of these embryos to develop to term was compared with control nuclear-transplant embryos in which t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90313-1
更新日期:1984-05-01 00:00:00
abstract::The presentation of peptides to T cells by MHC class II molecules is of critical importance in specific recognition by the immune system. Expression of class II molecules is exquisitely controlled at the transcriptional level. A large set of proteins interact with the promoters of class II genes. The most important of...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)00696-7
更新日期:2002-04-01 00:00:00
abstract::Malignancy can be suppressed by the immune system in a process termed immunosurveillance. However, to what extent immunosurveillance occurs in spontaneous cancers and the composition of participating cell types remains obscure. Here, we show that cell transformation triggers a tissue-resident lymphocyte response in on...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.01.002
更新日期:2016-01-28 00:00:00