Abstract：
:RECQL5 is the sole member of the RECQ family of helicases associated with RNA polymerase II (RNAPII). We now show that RECQL5 is a general elongation factor that is important for preserving genome stability during transcription. Depletion or overexpression of RECQL5 results in corresponding shifts in the genome-wide RNAPII density profile. Elongation is particularly affected, with RECQL5 depletion causing a striking increase in the average rate, concurrent with increased stalling, pausing, arrest, and/or backtracking (transcription stress). RECQL5 therefore controls the movement of RNAPII across genes. Loss of RECQL5 also results in the loss or gain of genomic regions, with the breakpoints of lost regions located in genes and common fragile sites. The chromosomal breakpoints overlap with areas of elevated transcription stress, suggesting that RECQL5 suppresses such stress and its detrimental effects, and thereby prevents genome instability in the transcribed region of genes.
journal_name
Celljournal_title
Cellauthors
Saponaro M,Kantidakis T,Mitter R,Kelly GP,Heron M,Williams H,Söding J,Stewart A,Svejstrup JQdoi
10.1016/j.cell.2014.03.048subject
Has Abstractpub_date
2014-05-22 00:00:00pages
1037-49issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(14)00477-2journal_volume
157pub_type
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