Abstract:
:The human La antigen is an RNA-binding protein that facilitates transcriptional termination and reinitiation by RNA polymerase III. Native La protein fractionates into transcriptionally active and inactive forms that are unphosphorylated and phosphorylated at serine 366, respectively, as determined by enzymatic and mass spectrometric analyses. Serine 366 comprises a casein kinase II phosphorylation site that resides within a conserved region in the La proteins from several species. RNA synthesis from isolated transcription complexes is inhibited by casein kinase II-mediated phosphorylation of La serine 366 and is reversible by dephosphorylation. This work demonstrates a novel mechanism of transcriptional control at the level of recycling of stable transcription complexes.
journal_name
Celljournal_title
Cellauthors
Fan H,Sakulich AL,Goodier JL,Zhang X,Qin J,Maraia RJdoi
10.1016/s0092-8674(00)81913-3subject
Has Abstractpub_date
1997-03-07 00:00:00pages
707-15issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81913-3journal_volume
88pub_type
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