Abstract:
:A yeast gene homologous to bacterial RNase III (RNT1) encodes a double-strand-specific endoribonuclease essential for ribosome synthesis. Two rRNA processing events are blocked in cells temperature sensitive for RNT1: cleavage at the snoRNA-dependent AO site in the 5' ETS and cleavage in the 3' ETS. Recombinant RNT1 protein accurately cleaves a synthetic 5' ETS RNA at AO site in vitro, in the absence of snoRNA or other factors. A synthetic 3' ETS substrate is specifically cleaved at a site 21 nt downstream of the 3' end 28S rRNA. These observations show that a protein endonuclease collaborates with snoRNAs in eukaryotic rRNA processing and exclude a catalytic role for snoRNAs at certain pre-rRNA cleavage.
journal_name
Celljournal_title
Cellauthors
Elela SA,Igel H,Ares M Jrdoi
10.1016/s0092-8674(00)81087-9subject
Has Abstractpub_date
1996-04-05 00:00:00pages
115-24issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81087-9journal_volume
85pub_type
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