Abstract：
:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in vivo primary transcripts. An ordered series of deletion clones, whose template sequences were truncated at either the 5' or 3' end, was used to identify the precise mitochondrial DNA sequence required for initiation of transcription. The data provide a definitive assignment of the promoter for heavy-strand transcription occurring within -16 to +7 of the transcriptional start site 16 nucleotides upstream of the 5' end of the gene for tRNAPhe and of the promoter for light-strand transcription occurring within -28 to +16 of the transcriptional start site at the 5' end of "7S RNA." Within each control sequence is a candidate promoter whose consensus sequence is 5'-CANACC(G)CC(A)AAAGAPyA-3' and in both cases transcriptional initiation occurs within six to eight nucleotides of the 3' end of this sequence. The transcriptional start site is an integral part of each promoter and each promoter can function in the absence of the other.
journal_name
Celljournal_title
Cellauthors
Chang DD,Clayton DAdoi
10.1016/0092-8674(84)90343-xsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
635-43issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(84)90343-Xjournal_volume
36pub_type
杂志文章相关文献
CELL文献大全abstract：:X-linked recessive chondrodysplasia punctata (CDPX) is a congenital defect of bone and cartilage development characterized by aberrant bone mineralization, severe underdevelopment of nasal cartilage, and distal phalangeal hypoplasia. A virtually identical phenotype is observed in the warfarin embryopathy, which is due...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90367-4
更新日期：1995-04-07 00:00:00
abstract：:Sharply delineated domains of cell types arise in developing tissues under instruction of inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. The translation of a morphogen gradient into discrete spatial domains relies on precise signal responses at stable cell positio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.03.023
更新日期：2013-04-25 00:00:00
abstract：:This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90026-6
更新日期：1979-03-01 00:00:00
abstract：:We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90391-7
更新日期：1995-05-05 00:00:00
abstract：:Muscle cell cultures from Drosophila melanogaster were obtained by plating dissociated gastrula stage embryo cells on protamine-treated culture dishes. They myogenic cells in these cultures fuse to form multinucleated pulsating cells by 15 hr after plating. An analysis of protein synthesis during myogenesis in these c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90210-6
更新日期：1978-04-01 00:00:00
abstract：:A mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step of transcription initiation, we used single-particle electron microscopy (EM) to visualize human TFIID with promoter DN...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.12.005
更新日期：2013-01-17 00:00:00
abstract：:The karyophilic properties of the viral matrix (MA) protein govern HIV nuclear import in nondividing cells such as macrophages. A critical regulator of this process is the C-terminal tyrosine phosphorylation of MA during virus maturation. Here, we reveal the mechanism of this phenomenon, by demonstrating that tyrosine...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90097-7
更新日期：1995-11-17 00:00:00
abstract：:Meyer et al. find that subjects lacking the AIRE gene, critical for self-tolerance in T lymphocytes, show a broad range of autoantibody specificities, which can have extremely high affinities. The data also suggest that some of these autoantibodies can, surprisingly, prevent some types of autoimmunity, particularly ty...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.07.018
更新日期：2016-07-28 00:00:00
abstract：:Sleep is characterized by behavioral quiescence, homeostasis, increased arousal threshold, and rapid reversibility. Understanding how these properties are encoded by a neuronal circuit has been difficult, and no single molecular or neuronal pathway has been shown to be responsible for the regulation of sleep. Taking a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.11.036
更新日期：2014-01-16 00:00:00
abstract：:The specificity of target gene activation by steroid receptors is encoded within a small, cysteine-rich domain that is believed to form two zinc-coordinated fingers. Here we show that the ability of glucocorticoid and estrogen receptors to discriminate between their closely related response elements resides in the two...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90050-0
更新日期：1989-06-30 00:00:00
abstract：:Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81269-6
更新日期：1996-06-14 00:00:00
abstract：:We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus results in the st...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90272-1
更新日期：1984-10-01 00:00:00
abstract：:Murine intracisternal A particles have a number of properties which are common to known RNA tumor viruses, but horizontal transmission has not been previously demonstrated. The apparent absence of infectivity may be related to the failure of these particles to be released from cisternae of endo-plasmic reticulum. Prev...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90083-0
更新日期：1976-11-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) develop from a specialized subpopulation of endothelial cells known as hemogenic endothelium (HE). Although the HE origin of HSCs is now well established in different species, the signaling pathways that control this transition remain poorly understood. Here, we show that activation of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.08.055
更新日期：2013-09-26 00:00:00
abstract：:Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.027
更新日期：2016-10-06 00:00:00
abstract：:I have defined conditions under which RepFIC plasmid DNA can be maintained in a state of lowered helical density. In exponentially growing cultures, the DNA of lowered helical density is present in small amounts but never totally absent, suggesting that it is a normal variant of plasmid maintenance. It is fully methyl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00402-0
更新日期：2001-06-29 00:00:00
abstract：:Most bacteria that colonize eukaryotes must bind directly to host cells to establish a replicative niche. In enteric bacteria, adhesion to host cells is often promoted by a lectin found on surface-localized pili. Some pili promote efficient adhesion only when they are subjected to shear stress, as found during the flo...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00821-8
更新日期：2002-07-12 00:00:00
abstract：:The enteric bacteria Salmonella typhimurium has the ability to invade (enter) nonphagocytic cells. The internalization process occurs as a result of an intimate interaction between the bacteria and the host cell, in which S. typhimurium triggers a cascade of host cell-signaling events leading to the formation of host ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90510-x
更新日期：1994-02-25 00:00:00
abstract：:In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to date, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Over...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81819-x
更新日期：1996-12-27 00:00:00
abstract：:torso encodes a receptor tyrosine kinase (torso) required for anterior and posterior terminal development of the Drosophila embryo. Injecting eggs with in vitro synthesized torso mRNAs revealed that torso activation is governed by an extracellular molecule, presumably the torso ligand, produced at terminal regions of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90394-r
更新日期：1992-12-11 00:00:00
abstract：:The dynamic synapse is represented by the constant mobility and exchange of components at both the cell surface and at intracellular sites. This includes thermally powered Brownian diffusion movement, followed by reversible trapping through receptor-scaffold interactions and active transport of cargo vesicles through ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.06.002
更新日期：2014-06-19 00:00:00
abstract：:Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory proce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.01.001
更新日期：2020-02-06 00:00:00
abstract：:Fos and Jun transcription factors are induced by a variety of extracellular signaling agents. We describe here an unusual member of the Fos family that is also induced, namely, a truncated form of FosB (delta FosB) missing the C-terminal 101 amino acids of FosB. delta FosB retains the dimerization and DNA-binding acti...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90504-r
更新日期：1991-02-22 00:00:00
abstract：:Neuronal plasticity depends on plasma membrane Ca(2+) influx, resulting in activity-dependent gene transcription. Calmodulin (CaM) activated by Ca(2+) initiates the nuclear events, but how CaM makes its way to the nucleus has remained elusive. Ma et al. now show that CaMKIIγ transports CaM from cell surface Ca(2+) cha...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2014.09.023
更新日期：2014-10-09 00:00:00
abstract：:X-linked lissencephaly and "double cortex" are allelic human disorders mapping to Xq22.3-Xq23 associated with arrest of migrating cerebral cortical neurons. We identified a novel 10 kb brain-specific cDNA interrupted by a balanced translocation in an XLIS patient that encodes a novel 40 kDa predicted protein named Dou...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80899-5
更新日期：1998-01-09 00:00:00
abstract：:Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.10.010
更新日期：2009-11-13 00:00:00
abstract：:Bacteria can respond to adverse environmental conditions by drastically reducing or even ceasing metabolic activity. They must then determine that conditions have improved before exiting dormancy, and one indication of such a change is the growth of other bacteria in the local environment. Growing bacteria release mur...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.08.039
更新日期：2008-10-31 00:00:00
abstract：:In yeast, hyperosmotic stress causes an immediate dissociation of most proteins from chromatin, presumably because cells are unprepared for, and initially unresponsive to, increased ion concentrations in the nucleus. Osmotic stress activates Hog1 MAP kinase, which phosphorylates at least two proteins located at the pl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.07.016
更新日期：2004-08-06 00:00:00
abstract：:Many cofactors bind the hormone-activated estrogen receptor (ER), yet the specific regulators of endogenous ER-mediated gene transcription are unknown. Using chromatin immunoprecipitation (ChIP), we find that ER and a number of coactivators rapidly associate with estrogen responsive promoters following estrogen treatm...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00188-4
更新日期：2000-12-08 00:00:00
abstract：:The treatment of advanced prostate cancer has been transformed by novel antiandrogen therapies such as enzalutamide. Here, we identify induction of glucocorticoid receptor (GR) expression as a common feature of drug-resistant tumors in a credentialed preclinical model, a finding also confirmed in patient samples. GR s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.11.012
更新日期：2013-12-05 00:00:00
