Abstract：
:This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage orientation axis is that which passes from the apex through the tail, the measured dichroism suggests that DNA is wrapped in closely packed, co-axial solenoids with the axis of the solenoids tipped 43.5 degrees +/- 2.5 degrees from the orientation axis of the phage. All three phages show a large permanent dipole moment, with respective values of 5600, 200,000 and 500,000 Debye for T7, phiCd-1 and P22. The radius of the equivalent sphere for the three phages calculated from the rotational relaxation time for the rise of dichroism is in agreement with birefringence and electron microscope observations. The circular dichroism spectra of all three bacteriophages indicate that the local DNA helicity is similar in each case.
journal_name
Celljournal_title
Cellauthors
Kosturko LD,Hogan M,Dattagupta Ndoi
10.1016/0092-8674(79)90026-6subject
Has Abstractpub_date
1979-03-01 00:00:00pages
515-22issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(79)90026-6journal_volume
16pub_type
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