Abstract：
:During Tetrahymena conjugation, programmed DNA degradation occurs in two separate nuclei. Thousands of germline-specific deletion elements are removed from the genome of the developing somatic macronucleus, and the old parental macronucleus is degraded by an apoptotic mechanism. An abundant polypeptide, Pdd1p (formerly p65), localizes to both of these nuclei at the time of DNA degradation. Here we report that, in developing macronuclei, Pdd1p localizes to electron-dense, heterochromatic structures that contain germline-specific deletion elements. Pdd1p also associates with parental macronuclei during terminal stages of apoptosis. Sequencing of the PDD1 gene reveals it to be a member of the chromodomain family, suggesting a molecular link between heterochromatin assembly and programmed DNA degradation.
journal_name
Celljournal_title
Cellauthors
Madireddi MT,Coyne RS,Smothers JF,Mickey KM,Yao MC,Allis CDdoi
10.1016/s0092-8674(00)81324-0subject
Has Abstractpub_date
1996-10-04 00:00:00pages
75-84issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81324-0journal_volume
87pub_type
杂志文章相关文献
CELL文献大全abstract：:CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that d...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.11.017
更新日期：2016-12-15 00:00:00
abstract：:p25rum1 from the fission yeast S. pombe is shown to act as a specific in vitro inhibitor of the p34cdc2/p56cdc13 mitotic kinase. It is also shown that early G1 cells contain p25rum1, which associates with and inhibits the mitotic kinase, and maintains p56cdc13 mitotic B cyclin at a low level, ensuring that these cells...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90215-5
更新日期：1995-12-15 00:00:00
abstract：:DNA demethylating agents are approved for some blood malignancies and are under active investigation in solid tumors, but how these drugs work has remained unclear. In this issue of Cell, two groups show that these agents activate a toxic cellular antiviral program through transcriptional activation of endogenous retr...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.08.005
更新日期：2015-08-27 00:00:00
abstract：:In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to date, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Over...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81819-x
更新日期：1996-12-27 00:00:00
abstract：:Following EMS mutagenesis we recovered a mutant of D. melanogaster that grows at concentrations of alpha-amanitin lethal to wild-type. To our knowledge this mutant represents the first example of an amanitin-resistant eucaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90116-8
更新日期：1979-11-01 00:00:00
abstract：:The anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 controls progression to S phase and mitosis by stabilizing key APC/C ubiquitination substrates, including cyclin A. Examining Emi1 binding proteins, we identified the Evi5 oncogene as a regulator of Emi1 accumulation. Evi5 antagonizes SCF(betaTrCP)-depend...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.10.038
更新日期：2006-01-27 00:00:00
abstract：:Choices have consequences. Immune cells survey and migrate throughout the body and sometimes take residence in niche environments with distinct communities of cells, extracellular matrix, and nutrients that may differ from those in which they matured. Imbedded in immune cell physiology are metabolic pathways and metab...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2017.04.004
更新日期：2017-05-04 00:00:00
abstract：:Certain tRNAs in S. cerevisiae (tRNATyr and tRNAPhe) arise via precursor molecules which are mature at the 5' and 3' termini but contain intervening sequences adjacent to the anticodon (Knapp et al., 1978; O'Farrell et al., 1978). In addition to these molecules, precursors to several other tRNAs accumulate in a temper...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90349-0
更新日期：1979-09-01 00:00:00
abstract：:We have isolated cDNA clones generated from the mRNA encoding the opsin apoprotein of bovine rhodopsin and used these cDNAs to isolate genomic DNA clones containing the complete opsin gene. Nucleotide sequence analysis of the cloned DNAs has yielded a complete amino acid sequence for bovine rhodopsin and provided an i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90537-8
更新日期：1983-10-01 00:00:00
abstract：:Tracing the lineage history of cells is key to answering diverse and fundamental questions in biology. Coupling of cell ancestry information with other molecular readouts represents an important goal in the field. Here, we describe the CRISPR array repair lineage tracing (CARLIN) mouse line and corresponding analysis ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.04.048
更新日期：2020-06-11 00:00:00
abstract：:Regulated proteolysis is essential for cell cycle progression in both prokaryotes and eukaryotes. We show here that the ClpXP protease, responsible for the degradation of multiple bacterial proteins, is dynamically localized to specific cellular positions in Caulobacter where it degrades colocalized proteins. The CtrA...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.12.033
更新日期：2006-02-10 00:00:00
abstract：:The neurofibromatosis type 1 (NF1) protein contains a region of significant sequence similarity to ras p21 GTPase-activating protein (GAP) and the yeast IRA1 gene product. A fragment of NF1 cDNA encoding the GAP-related domain (NF1 GRD) was expressed, immunoaffinity purified, and assayed for effects on N-ras p21 GTPas...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90150-d
更新日期：1990-11-16 00:00:00
abstract：:V(D)J recombination of antigen receptor gene segments in B and T cells is mediated by the lymphoid-specific proteins RAG1 and RAG2. Now, Ji et al. (2010) demonstrate how RAG1 and RAG2 use DNA sequence specificity and modified histones within chromatin to target specific loci for V(D)J recombination at different stages...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.04.014
更新日期：2010-04-30 00:00:00
abstract：:Metastasis is a rare event. Does it arise from rare, variant, highly metastatic cells or does a primary tumor progress to a premalignant state from which metastases arise stochastically without further changes in gene expression? Arguments and evidence have been adduced to support either position. A paper in this mont...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(03)00468-9
更新日期：2003-06-27 00:00:00
abstract：:Synonymous mutations change the sequence of a gene without directly altering the sequence of the encoded protein. Here, we present evidence that these "silent" mutations frequently contribute to human cancer. Selection on synonymous mutations in oncogenes is cancer-type specific, and although the functional consequenc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.01.051
更新日期：2014-03-13 00:00:00
abstract：:Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.12.019
更新日期：2017-01-12 00:00:00
abstract：:We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90391-7
更新日期：1995-05-05 00:00:00
abstract：:p120-catenin regulates cell-cell adhesion by controlling cell surface retention of cadherin. In this issue, Ishiyama et al. (2010) present the first crystal structure of p120 in complex with cadherin, revealing molecular details of the functional interface and providing sophisticated new tools for dissecting p120's ro...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.03.022
更新日期：2010-04-02 00:00:00
abstract：:TET-mediated 5-methyl cytosine (5mC) oxidation acts in epigenetic regulation, stem cell development, and cancer. Hu et al. now determine the crystal structure of the TET2 catalytic domain bound to DNA, shedding light on 5mC-DNA substrate recognition and the catalytic mechanism of 5mC oxidation. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2013.12.002
更新日期：2013-12-19 00:00:00
abstract：:Metabolic activity is intimately linked to T cell fate and function. Using high-resolution mass spectrometry, we generated dynamic metabolome and proteome profiles of human primary naive T cells following activation. We discovered critical changes in the arginine metabolism that led to a drop in intracellular L-argini...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.031
更新日期：2016-10-20 00:00:00
abstract：:In a 4-cell stage C. elegans embryo, signaling by the P2 blastomere induces anterior-posterior polarity in the adjacent EMS blastomere, leading to endoderm formation. We have taken genetic and reverse genetic approaches toward understanding the molecular basis for this induction. These studies have identified a set of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80531-0
更新日期：1997-08-22 00:00:00
abstract：:Mutually exclusive expression within the var gene family of the malaria parasite Plasmodium falciparum is important for parasite survival and virulence. In this issue of Cell, Duraisingh et al. and Freitas-Junior et al. provide evidence for the role of Sir2-dependent alterations in chromatin structure and changes in s...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2005.03.019
更新日期：2005-04-08 00:00:00
abstract：:The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical prope...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.05.038
更新日期：2011-06-24 00:00:00
abstract：:Macroautophagy is a lysosomal degradative pathway essential for neuron survival. Here, we show that macroautophagy requires the Alzheimer's disease (AD)-related protein presenilin-1 (PS1). In PS1 null blastocysts, neurons from mice hypomorphic for PS1 or conditionally depleted of PS1, substrate proteolysis and autopha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.05.008
更新日期：2010-06-25 00:00:00
abstract：:The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90278-6
更新日期：1981-01-01 00:00:00
abstract：:Neurodegenerative diseases can occur so early as to affect neurodevelopment. From a cohort of more than 2,000 consanguineous families with childhood neurological disease, we identified a founder mutation in four independent pedigrees in cleavage and polyadenylation factor I subunit 1 (CLP1). CLP1 is a multifunctional ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.03.049
更新日期：2014-04-24 00:00:00
abstract：:We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90450-1
更新日期：1984-12-01 00:00:00
abstract：:In this issue, Wilson et al. (2008) demonstrate that there are two functional subsets of hematopoietic stem cells that have distinctive kinetics of cell cycling. They present evidence that cells may transition between the two kinetic states, establishing one subpopulation that is ready to proliferate and another that ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.11.034
更新日期：2008-12-12 00:00:00
abstract：:Notch signaling is broadly used to regulate cell-fate decisions. We have identified a gene, rumi, with a temperature-sensitive Notch phenotype. At 28 degrees C-30 degrees C, rumi clones exhibit a full-blown loss of Notch signaling in all tissues tested. However, at 18 degrees C only a mild Notch phenotype is evident. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.12.016
更新日期：2008-01-25 00:00:00
abstract：:In the nucleus of eukaryotic cells, RNA decay in the 3'-5' direction is carried out by a complex of exonucleases called the exosome. Surprisingly, the purified exosome shows only weak activity in vitro. Two papers in this issue of Cell, by LaCava et al. (2005) and Wyers et al. (2005), and a third report by Vanacova et...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2005.05.018
更新日期：2005-06-03 00:00:00
