Abstract：
:Following EMS mutagenesis we recovered a mutant of D. melanogaster that grows at concentrations of alpha-amanitin lethal to wild-type. To our knowledge this mutant represents the first example of an amanitin-resistant eucaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA-dependent RNA polymerase II, which is approximately 250 times less sensitive to inhibition by amanitin than the wild-type polymerase II whether tested in nuclei, in partially-fractionated extracts or as a highly purified enzyme. While the wild-type enzyme activity is inhibited 50% by 2.1 x 10(-8) M alpha-amanitin, inhibition of 50% of the AmaC4 RNA polymerase II activity requires a toxin concentration of 5.6 x 10(-6) M. The mutation responsible for the amanitin resistance of AmaC4 is on the X chromosome near the vermillion locus.
journal_name
Celljournal_title
Cellauthors
Greenleaf AL,Borsett LM,Jiamachello PF,Coulter DEdoi
10.1016/0092-8674(79)90116-8subject
Has Abstractpub_date
1979-11-01 00:00:00pages
613-22issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(79)90116-8journal_volume
18pub_type
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