9-cis retinoic acid is a high affinity ligand for the retinoid X receptor.


:All-trans retinoic acid (RA) has previously been shown to modulate the transcriptional properties of the retinoic acid receptor (RAR) and retinoid X receptor (RXR). The inability of all-trans RA to bind to RXR suggests that it may be metabolized to a more active high affinity ligand. We report here an experimental approach that has identified 9-cis RA as an RXR ligand. It is up to 40-fold more potent than all-trans RA in transfection assays and binds with high affinity. The production of 9-cis RA in cultured cells and the identification of this molecule in liver and kidney demonstrates the existence of this molecule in living organisms. The discovery of this novel hormone points to the key role retinoid metabolism may have in generating new signaling pathways.






Heyman RA,Mangelsdorf DJ,Dyck JA,Stein RB,Eichele G,Evans RM,Thaller C




Has Abstract


1992-01-24 00:00:00
















  • The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site.

    abstract::The Escherichia coli relBE operon encodes a toxin-antitoxin pair, RelE-RelB. RelB can reverse inhibition of protein synthesis by RelE in vivo. We have found that although RelE does not degrade free RNA, it cleaves mRNA in the ribosomal A site with high codon specificity. Among stop codons UAG is cleaved with fast, UAA...


    pub_type: 杂志文章


    authors: Pedersen K,Zavialov AV,Pavlov MY,Elf J,Gerdes K,Ehrenberg M

    更新日期:2003-01-10 00:00:00

  • Characterization of Op3, a lysis-defective mutant of bacteriophage f2.

    abstract::We have isolated a conditional lethal mutant of bacteriophage 12 which makes plaques only on E. coli strains carrying a UGA suppressor. It grows normally in nonsuppressing hosts but does not lyse such strains. The mutation complements with amber mutations in each of the three known phage cistrons. These observations l...


    pub_type: 杂志文章


    authors: Model P,Webster RE,Zinder ND

    更新日期:1979-10-01 00:00:00

  • Bicoid by the numbers: quantifying a morphogen gradient.

    abstract::Morphogen gradients are typically analyzed from static images of fixed embryonic tissues. Two papers in this issue of Cell now report live imaging of the Bicoid gradient in developing fruit fly embryos (Gregor et al., 2007a, 2007b). Their findings indicate that the gradient is highly reproducible from embryo to embryo...


    pub_type: 评论,杂志文章


    authors: Gibson MC

    更新日期:2007-07-13 00:00:00

  • Rosalind Franklin and the Advent of Molecular Biology.

    abstract::Rosalind Franklin provided the key data for deriving the double helix structure of DNA. The English chemist also pioneered structural studies of colloids, viruses, and RNA. To celebrate the 100th anniversary of Franklin's birth, I summarize her work, which shaped the emerging discipline of molecular biology. ...


    pub_type: 杂志文章


    authors: Cramer P

    更新日期:2020-08-20 00:00:00

  • Structure-guided reprogramming of human cGAS dinucleotide linkage specificity.

    abstract::Cyclic dinucleotides (CDNs) play central roles in bacterial pathogenesis and innate immunity. The mammalian enzyme cGAS synthesizes a unique cyclic dinucleotide (cGAMP) containing a 2'-5' phosphodiester linkage essential for optimal immune stimulation, but the molecular basis for linkage specificity is unknown. Here, ...


    pub_type: 杂志文章


    authors: Kranzusch PJ,Lee ASY,Wilson SC,Solovykh MS,Vance RE,Berger JM,Doudna JA

    更新日期:2014-08-28 00:00:00

  • Completion of mouse embryogenesis requires both the maternal and paternal genomes.

    abstract::Transplantation of pronuclei between one-cell-stage embryos was used to construct diploid mouse embryos with two female pronuclei ( biparental gynogenones ) or two male pronuclei ( biparental androgenones ). The ability of these embryos to develop to term was compared with control nuclear-transplant embryos in which t...


    pub_type: 杂志文章


    authors: McGrath J,Solter D

    更新日期:1984-05-01 00:00:00

  • Clonal lethality caused by the yeast plasmid 2 mu DNA.

    abstract::Strains of Saccharomyces that carry the nib allele of a nuclear gene exhibit a "nibbled" colony morphology if they also harbor the plasmid 2 mu DNA. I have found that the expression of the nibbled phenotype is correlated with the presence of a subpopulation of abnormally large cells that give rise to mortal clones. La...


    pub_type: 杂志文章


    authors: Holm C

    更新日期:1982-06-01 00:00:00

  • Synthesis of H1 histones by BHK cells in G1.

    abstract::The synthesis of histones and DNA was examined in BHK cells arrested in G1 by isoleucine starvation and in cells progressing into the S phase upon isoleucine refeeding. Approximately 2-3% of the cells were not arrested in G1 and synthesized DNA. The rate of synthesis of DNA and nucleosomal histones observed in cells s...


    pub_type: 杂志文章


    authors: Tarnowka MA,Baglioni C,Basilico C

    更新日期:1978-09-01 00:00:00

  • Reducing the mystery of neuronal differentiation.

    abstract::In the developing nervous system, neural progenitors exit the cell cycle and differentiate on a precise schedule, yet the mechanisms driving this process remain poorly defined. Yan et al. (2009) now identify a thiol-redox reaction mediated by the membrane protein GDE2 and the peroxiredoxin protein Prdx1 that promotes ...


    pub_type: 评论,杂志文章


    authors: Novitch BG,Butler SJ

    更新日期:2009-09-18 00:00:00

  • Organoid models of human and mouse ductal pancreatic cancer.

    abstract::Pancreatic cancer is one of the most lethal malignancies due to its late diagnosis and limited response to treatment. Tractable methods to identify and interrogate pathways involved in pancreatic tumorigenesis are urgently needed. We established organoid models from normal and neoplastic murine and human pancreas tiss...


    pub_type: 杂志文章


    authors: Boj SF,Hwang CI,Baker LA,Chio II,Engle DD,Corbo V,Jager M,Ponz-Sarvise M,Tiriac H,Spector MS,Gracanin A,Oni T,Yu KH,van Boxtel R,Huch M,Rivera KD,Wilson JP,Feigin ME,Öhlund D,Handly-Santana A,Ardito-Abraham CM,L

    更新日期:2015-01-15 00:00:00

  • The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.

    abstract::The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amoun...


    pub_type: 杂志文章


    authors: Kurilla MG,Keene JD

    更新日期:1983-10-01 00:00:00

  • Fusion of the Escherichia coli tRNALeu1 promoter to the galK gene: analysis of sequences necessary for growth-rate-dependent regulation.

    abstract::We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...


    pub_type: 杂志文章


    authors: Duester G,Elford RM,Holmes WM

    更新日期:1982-10-01 00:00:00

  • Differential response of multiple epsilon-globin cap sites to cis- and trans-acting controls.

    abstract::The human epsilon-globin gene has a number of alternative transcription-initiation sites located upstream of the canonical mRNA cap site. In three nonerythroid cell lines, "leaky" epsilon-globin transcription occurs exclusively from one of these upstream sites, the -200 cap site. Using a transient expression assay, we...


    pub_type: 杂志文章


    authors: Allan M,Zhu JD,Montague P,Paul J

    更新日期:1984-09-01 00:00:00

  • N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency.

    abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...


    pub_type: 杂志文章


    authors: Wang X,Zhao BS,Roundtree IA,Lu Z,Han D,Ma H,Weng X,Chen K,Shi H,He C

    更新日期:2015-06-04 00:00:00

  • In vivo analysis of Drosophila bicoid mRNA localization reveals a novel microtubule-dependent axis specification pathway.

    abstract::Drosophila bicoid mRNA is synthesized in the nurse cells and transported to the oocyte where microtubules and Exuperantia protein mediate localization to the anterior pole. Fluorescent bicoid mRNA injected into the oocyte displays nonpolar microtubule-dependent transport to the closest cortical surface, and the oocyte...


    pub_type: 杂志文章


    authors: Cha BJ,Koppetsch BS,Theurkauf WE

    更新日期:2001-07-13 00:00:00

  • A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response.

    abstract::Recently, more than 1000 large intergenic noncoding RNAs (lincRNAs) have been reported. These RNAs are evolutionarily conserved in mammalian genomes and thus presumably function in diverse biological processes. Here, we report the identification of lincRNAs that are regulated by p53. One of these lincRNAs (lincRNA-p21...


    pub_type: 杂志文章


    authors: Huarte M,Guttman M,Feldser D,Garber M,Koziol MJ,Kenzelmann-Broz D,Khalil AM,Zuk O,Amit I,Rabani M,Attardi LD,Regev A,Lander ES,Jacks T,Rinn JL

    更新日期:2010-08-06 00:00:00

  • Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.

    abstract::X-linked lissencephaly and "double cortex" are allelic human disorders mapping to Xq22.3-Xq23 associated with arrest of migrating cerebral cortical neurons. We identified a novel 10 kb brain-specific cDNA interrupted by a balanced translocation in an XLIS patient that encodes a novel 40 kDa predicted protein named Dou...


    pub_type: 杂志文章


    authors: Gleeson JG,Allen KM,Fox JW,Lamperti ED,Berkovic S,Scheffer I,Cooper EC,Dobyns WB,Minnerath SR,Ross ME,Walsh CA

    更新日期:1998-01-09 00:00:00

  • Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome.

    abstract::Nijmegen breakage syndrome (NBS) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. Cells from NBS patients are hypersensitive to ionizing radiation with cytogenetic features indistinguishable from ataxia telangiect...


    pub_type: 杂志文章


    authors: Varon R,Vissinga C,Platzer M,Cerosaletti KM,Chrzanowska KH,Saar K,Beckmann G,Seemanová E,Cooper PR,Nowak NJ,Stumm M,Weemaes CM,Gatti RA,Wilson RK,Digweed M,Rosenthal A,Sperling K,Concannon P,Reis A

    更新日期:1998-05-01 00:00:00

  • Cancer Immunosurveillance by Tissue-Resident Innate Lymphoid Cells and Innate-like T Cells.

    abstract::Malignancy can be suppressed by the immune system in a process termed immunosurveillance. However, to what extent immunosurveillance occurs in spontaneous cancers and the composition of participating cell types remains obscure. Here, we show that cell transformation triggers a tissue-resident lymphocyte response in on...


    pub_type: 杂志文章


    authors: Dadi S,Chhangawala S,Whitlock BM,Franklin RA,Luo CT,Oh SA,Toure A,Pritykin Y,Huse M,Leslie CS,Li MO

    更新日期:2016-01-28 00:00:00

  • Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains.

    abstract::The low-complexity (LC) domains of the products of the fused in sarcoma (FUS), Ewings sarcoma (EWS), and TAF15 genes are translocated onto a variety of different DNA-binding domains and thereby assist in driving the formation of cancerous cells. In the context of the translocated fusion proteins, these LC sequences fu...


    pub_type: 杂志文章


    authors: Kwon I,Kato M,Xiang S,Wu L,Theodoropoulos P,Mirzaei H,Han T,Xie S,Corden JL,McKnight SL

    更新日期:2013-11-21 00:00:00

  • Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.

    abstract::Complex gene regulatory networks require transcription factors (TFs) to bind distinct DNA sequences. To understand how novel TF specificity evolves, we combined phylogenetic, biochemical, and biophysical approaches to interrogate how DNA recognition diversified in the steroid hormone receptor (SR) family. After duplic...


    pub_type: 杂志文章


    authors: McKeown AN,Bridgham JT,Anderson DW,Murphy MN,Ortlund EA,Thornton JW

    更新日期:2014-09-25 00:00:00

  • A complexin/synaptotagmin 1 switch controls fast synaptic vesicle exocytosis.

    abstract::Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that have been primed for release by SNARE-complex assembly. Besides synaptotagmin 1, fast Ca(2+)-triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their ...


    pub_type: 杂志文章


    authors: Tang J,Maximov A,Shin OH,Dai H,Rizo J,Südhof TC

    更新日期:2006-09-22 00:00:00

  • A role for tRNA modifications in genome structure and codon usage.

    abstract::Transfer RNA (tRNA) gene content is a differentiating feature of genomes that contributes to the efficiency of the translational apparatus, but the principles shaping tRNA gene copy number and codon composition are poorly understood. Here, we report that the emergence of two specific tRNA modifications shaped the stru...


    pub_type: 杂志文章


    authors: Novoa EM,Pavon-Eternod M,Pan T,Ribas de Pouplana L

    更新日期:2012-03-30 00:00:00

  • E. coli mutant pleiotropically defective in the export of secreted proteins.

    abstract::A hybrid beta-galactosidase molecule containing a substantial portion of the amino-terminal sequence of the maltose-binding protein is inserted in the cytoplasmic membrane of E. coli; in this location, the protein has very low enzymatic activity. The strain producing it is, therefore, Lac-. Selection for derivatives o...


    pub_type: 杂志文章


    authors: Oliver DB,Beckwith J

    更新日期:1981-09-01 00:00:00

  • Disconnected: a locus required for neuronal pathway formation in the visual system of Drosophila.

    abstract::Mutations at the X-linked disconnected locus of D. melanogaster lead to the failure of adult photoreceptor axons to innervate their target cells in the developing optic lobes of the third instar larva, resulting in flies that have rudimentary optic ganglia. The cascade of epigenetic events leading to the adult disconn...


    pub_type: 杂志文章


    authors: Steller H,Fischbach KF,Rubin GM

    更新日期:1987-09-25 00:00:00

  • Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18).

    abstract::We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule...


    pub_type: 杂志文章


    authors: Lee JO,Rieu P,Arnaout MA,Liddington R

    更新日期:1995-02-24 00:00:00

  • Trans-activation by HIV-1 Tat via a heterologous RNA binding protein.

    abstract::The HIV-1 trans-activator Tat increases levels of viral gene expression and replication. The target for Tat is an RNA stem-loop called TAR, located at the 5' end of all viral transcripts. To study the mechanism of action and map functional domains of Tat, we fused Tat to the coat protein of bacteriophage MS2, an RNA b...


    pub_type: 杂志文章


    authors: Selby MJ,Peterlin BM

    更新日期:1990-08-24 00:00:00

  • Complement C1q activates canonical Wnt signaling and promotes aging-related phenotypes.

    abstract::Wnt signaling plays critical roles in development of various organs and pathogenesis of many diseases, and augmented Wnt signaling has recently been implicated in mammalian aging and aging-related phenotypes. We here report that complement C1q activates canonical Wnt signaling and promotes aging-associated decline in ...


    pub_type: 杂志文章


    authors: Naito AT,Sumida T,Nomura S,Liu ML,Higo T,Nakagawa A,Okada K,Sakai T,Hashimoto A,Hara Y,Shimizu I,Zhu W,Toko H,Katada A,Akazawa H,Oka T,Lee JK,Minamino T,Nagai T,Walsh K,Kikuchi A,Matsumoto M,Botto M,Shiojima

    更新日期:2012-06-08 00:00:00

  • Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.

    abstract::Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova tel...


    pub_type: 杂志文章


    authors: Horvath MP,Schweiker VL,Bevilacqua JM,Ruggles JA,Schultz SC

    更新日期:1998-12-23 00:00:00

  • Glial lipid droplets and ROS induced by mitochondrial defects promote neurodegeneration.

    abstract::Reactive oxygen species (ROS) and mitochondrial defects in neurons are implicated in neurodegenerative disease. Here, we find that a key consequence of ROS and neuronal mitochondrial dysfunction is the accumulation of lipid droplets (LD) in glia. In Drosophila, ROS triggers c-Jun-N-terminal Kinase (JNK) and Sterol Reg...


    pub_type: 杂志文章


    authors: Liu L,Zhang K,Sandoval H,Yamamoto S,Jaiswal M,Sanz E,Li Z,Hui J,Graham BH,Quintana A,Bellen HJ

    更新日期:2015-01-15 00:00:00