Abstract:
:We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production of cAMP following stimulation by an activated RAS allele. Null mutations of srv2 result in lethality, which cannot be suppressed by mutational activation of the cAMP-dependent protein kinase. The sequence of the gene indicates that it encodes an adenylate cyclase-associated protein. These results demonstrate that SRV2 protein is required for RAS-activated adenylate cyclase activity, but that it participates in other essential cellular functions as well.
journal_name
Celljournal_title
Cellauthors
Fedor-Chaiken M,Deschenes RJ,Broach JRdoi
10.1016/0092-8674(90)90813-tsubject
Has Abstractpub_date
1990-04-20 00:00:00pages
329-40issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(90)90813-Tjournal_volume
61pub_type
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