Abstract:
:Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated CaMKII phenocopied structural abnormalities of dlg mutant synapses and dramatically increased extrajunctional DLG. Decreased CaMKII activity caused opposite alterations. In vitro, CaMKII phosphorylated a DLG fragment with a stoichiometry close to one. Moreover, expression of site-directed dlg mutants that blocked or mimicked phosphorylation had effects similar to those observed upon inhibiting or constitutively activating CaMKII. We propose that CaMKII-dependent DLG phosphorylation regulates the association of DLG with the synaptic complex during development and plasticity, thus providing a link between synaptic activity and structure.
journal_name
Celljournal_title
Cellauthors
Koh YH,Popova E,Thomas U,Griffith LC,Budnik Vdoi
10.1016/s0092-8674(00)81964-9subject
Has Abstractpub_date
1999-08-06 00:00:00pages
353-63issue
3eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81964-9journal_volume
98pub_type
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