Abstract：
:Notch signaling is broadly used to regulate cell-fate decisions. We have identified a gene, rumi, with a temperature-sensitive Notch phenotype. At 28 degrees C-30 degrees C, rumi clones exhibit a full-blown loss of Notch signaling in all tissues tested. However, at 18 degrees C only a mild Notch phenotype is evident. In vivo analyses reveal that the target of Rumi is the extracellular domain of Notch. Notch accumulates intracellularly and at the cell membrane of rumi cells but fails to be properly cleaved, despite normal binding to Delta. Rumi is an endoplasmic reticulum-retained protein with a highly conserved CAP10 domain. Our studies show that Rumi is a protein O-glucosyltransferase, capable of adding glucose to serine residues in Notch EGF repeats with the consensus C1-X-S-X-P-C2 sequence. These data indicate that by O-glucosylating Notch in the ER, Rumi regulates its folding and/or trafficking and allows signaling at the cell membrane.
journal_name
Celljournal_title
Cellauthors
Acar M,Jafar-Nejad H,Takeuchi H,Rajan A,Ibrani D,Rana NA,Pan H,Haltiwanger RS,Bellen HJdoi
10.1016/j.cell.2007.12.016subject
Has Abstractpub_date
2008-01-25 00:00:00pages
247-58issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(07)01617-0journal_volume
132pub_type
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