Abstract：
:Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane during translocation, successive segments of the chain are incrementally exposed to solvent and yet are unlikely to be able to fold. This topological restriction on folding likely mandates a need for PCB proteins to prevent aggregation. Catalysis of topologically restricted folding by PCB proteins is likely to involve both an antifolding activity that postpones folding until entire domains are available and, more speculatively, a folding activity resulting from a programmed stepwise release that employs the energy of ATP hydrolysis to ensure a favorable pathway. We are left with a new set of problems. How do proteins fold in cells? What are the sequences or structural signals that dictate folding pathways? The new challenge will be to understand folding as a combination of physical chemistry, enzymology, and cell biology.
journal_name
Celljournal_title
Cellauthors
Rothman JEdoi
10.1016/0092-8674(89)90005-6subject
Has Abstractpub_date
1989-11-17 00:00:00pages
591-601issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(89)90005-6journal_volume
59pub_type
杂志文章,评审相关文献
CELL文献大全abstract：:In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90066-7
更新日期：1979-10-01 00:00:00
abstract：:Secreted proteins of the Wnt family have profound organizing roles during animal development and are transduced via the activities of the Frizzled (Fz) class of transmembrane receptors and the TCF/LEF/Pangolin class of transcription factors. beta-catenins, including Drosophila Armadillo (Arm), link activation of Fz at...
journal_title：Cell
pub_type： 杂志文章,收录出版
doi：10.1016/s0092-8674(02)01037-1
更新日期：2002-10-18 00:00:00
abstract：:Receptor-interacting protein kinase (RIPK)-1 is involved in RIPK3-dependent and -independent signaling pathways leading to cell death and/or inflammation. Genetic ablation of ripk1 causes postnatal lethality, which was not prevented by deletion of ripk3, caspase-8, or fadd. However, animals that lack RIPK1, RIPK3, and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.04.018
更新日期：2014-05-22 00:00:00
abstract：:Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.10.050
更新日期：2012-01-20 00:00:00
abstract：:The PML tumor suppressor controls key pathways for growth suppression, induction of apoptosis, and cellular senescence. PML loss occurs frequently in human tumors through unknown posttranslational mechanisms. Casein kinase 2 (CK2) is oncogenic and frequently upregulated in human tumors. Here we show that CK2 regulates...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.05.041
更新日期：2006-07-28 00:00:00
abstract：:Inflammation is associated with many neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. In this Review, we discuss inducers, sensors, transducers, and effectors of neuroinflammation that contribute to neuronal dysfunction and death. Al...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2010.02.016
更新日期：2010-03-19 00:00:00
abstract：:The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80875-2
更新日期：2000-06-09 00:00:00
abstract：:A nutritious diet low in calories improves the health and extends the life span of rodents. Recent studies identified a gene, SIR2, which encodes an NAD-dependent deacetylase and may mediate the effects of calorie restriction. In this review, we discuss SIR2 genes and calorie restriction in the lower organisms yeast a...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2005.01.029
更新日期：2005-02-25 00:00:00
abstract：:We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90245-4
更新日期：1985-12-01 00:00:00
abstract：:Vertebrate Hoxd genes are sequentially activated during the morphogenesis and pattern formation of the limb. Using the approach of gene disruption via homologous recombination in embryonic stem cells, we have assessed the function of the last gene of the complex, Hoxd-13. Mutant mice displayed skeletal alterations alo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90378-4
更新日期：1993-11-05 00:00:00
abstract：:In this issue of Cell, Noma et al. (2006) show that B-boxes and TFIIIC limit the spread of heterochromatin at the silent mat region in the fission yeast genome. Global analysis of TFIIIC distribution revealed dispersed sites of association that coalesce at the nuclear periphery, suggesting that TFIIIC may act as a bar...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.05.016
更新日期：2006-06-02 00:00:00
abstract：:Misfolded proteins accumulating in several neurodegenerative diseases (including Alzheimer, Parkinson, and Huntington diseases) can cause aggregation of their native counterparts through a mechanism similar to the infectious prion protein's induction of a pathogenic conformation onto its cellular isoform. Evidence for...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.10.011
更新日期：2011-10-28 00:00:00
abstract：:In 1981, the acquired immunodeficiency syndrome (AIDS) appeared insidiously and mystified doctors and scientists alike. No one could have predicted then that it would become, arguably, the worst plague in human history. Today, 33 million persons are living with infection by the human immunodeficiency virus type 1 (HIV...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.05.003
更新日期：2008-05-16 00:00:00
abstract：:The intermediate filament proteins, desmin and vimentin, are phosphorylated in skeletal muscle cells in vivo. Desmin kinase activities have been purified from mature chicken skeletal muscle and identified as the cAMP-dependent kinases. Chicken skeletal muscle contains two cAMP-dependent protein kinases which are simil...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90278-6
更新日期：1981-01-01 00:00:00
abstract：:We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). The structure reveals an extended rod-like molecule with a long axis of approximately 140 A and highly variable relationships between adjacent domains. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81002-8
更新日期：1996-01-12 00:00:00
abstract：:Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00986-3
更新日期：2003-12-26 00:00:00
abstract：:Mouse plasmacytoma (MOPC) 460 cells contain two chromatographic forms of RNA polymerase III (IIIA and IIIB) in addition to the major class I and II RNA polymerases. Nuclei isolated from these cells actively synthesize RNA. Among the discrete transcription products observed are the 5S and 4.5S RNAs and additional low m...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90028-9
更新日期：1977-03-01 00:00:00
abstract：:When treated with the synthetic glucocorticoid dexamethasone, HT1080 human fibrosarcoma cells show changes in morphology, adhesion, and the extracellular matrix. Dexamethasone treatment results in a tenfold increase in the rate of fibronectin biosynthesis in HT1080 cells and a twofold increase in untransformed, normal...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90357-4
更新日期：1983-05-01 00:00:00
abstract：:In order for telomeres to remain functional and stable, they must rendezvous with the enzyme telomerase in a productive manner. In human cells, this interaction is mediated by Cajal bodies as matchmaker, and now Zhong et al. reveal molecular determinants that establish good chemistry between the two partners. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2012.07.015
更新日期：2012-08-03 00:00:00
abstract：:We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90290-2
更新日期：1982-10-01 00:00:00
abstract：:We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00700-6
更新日期：2002-04-19 00:00:00
abstract：:We propose a mechanism for the priming of influenza viral RNA transcription by capped RNAs in which specific 5'-terminal fragments are cleaved from the capped RNAs by a virion-associated endonuclease. These fragments would serve as the actual primers for the initiation of transcription by the initial incorporation by ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90449-9
更新日期：1981-03-01 00:00:00
abstract：:Many cofactors bind the hormone-activated estrogen receptor (ER), yet the specific regulators of endogenous ER-mediated gene transcription are unknown. Using chromatin immunoprecipitation (ChIP), we find that ER and a number of coactivators rapidly associate with estrogen responsive promoters following estrogen treatm...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00188-4
更新日期：2000-12-08 00:00:00
abstract：:In addition to xeroderma pigmentosum, mutations in the human XPG gene cause early onset Cockayne syndrome (CS). Here, we provide evidence for the involvement of RAD2, the S. cerevisiae counterpart of XPG, in promoting efficient RNA polymerase II transcription. Inactivation of RAD26, the S. cerevisiae counterpart of th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00795-x
更新日期：2002-06-28 00:00:00
abstract：:Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90502-0
更新日期：1983-01-01 00:00:00
abstract：:Choices have consequences. Immune cells survey and migrate throughout the body and sometimes take residence in niche environments with distinct communities of cells, extracellular matrix, and nutrients that may differ from those in which they matured. Imbedded in immune cell physiology are metabolic pathways and metab...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2017.04.004
更新日期：2017-05-04 00:00:00
abstract：:Signaling by the epidermal growth factor receptor requires an allosteric interaction between the kinase domains of two receptors, whereby one activates the other. We show that the intracellular juxtamembrane segment of the receptor, known to potentiate kinase activity, is able to dimerize the kinase domains. The C-ter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.04.025
更新日期：2009-06-26 00:00:00
abstract：:Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80592-9
更新日期：1999-03-19 00:00:00
abstract：:The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tiss...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)01087-5
更新日期：2002-11-27 00:00:00
abstract：:Ovule development in Arabidopsis involves the formation of three morphologically defined proximal-distal pattern elements. Integuments arise from the central pattern element. Analysis of Bell 1 (Bel 1) mutant ovules indicated that BEL1 was required for integument development. Cloning of the BEL1 locus reveals that it ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90186-8
更新日期：1995-12-01 00:00:00
