Abstract:
:In addition to xeroderma pigmentosum, mutations in the human XPG gene cause early onset Cockayne syndrome (CS). Here, we provide evidence for the involvement of RAD2, the S. cerevisiae counterpart of XPG, in promoting efficient RNA polymerase II transcription. Inactivation of RAD26, the S. cerevisiae counterpart of the human CSB gene, also causes a deficiency in transcription, and a synergistic decline in transcription occurs in the absence of both the RAD2 and RAD26 genes. Growth is also retarded in the rad2 Delta and rad26 Delta single mutant strains, and a very severe growth inhibition is seen in the rad2 Delta rad26 Delta double mutant. From these and other observations presented here, we suggest that transcriptional defects are the underlying cause of CS.
journal_name
Celljournal_title
Cellauthors
Lee SK,Yu SL,Prakash L,Prakash Sdoi
10.1016/s0092-8674(02)00795-xsubject
Has Abstractpub_date
2002-06-28 00:00:00pages
823-34issue
7eissn
0092-8674issn
1097-4172pii
S009286740200795Xjournal_volume
109pub_type
杂志文章相关文献
CELL文献大全abstract::During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the fla...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90073-4
更新日期:1979-10-01 00:00:00
abstract::The Drosophila transforming growth factor beta (TGF-beta) homolog Decapentaplegic (Dpp) acts as a morphogen that forms a long-range concentration gradient to direct the anteroposterior patterning of the wing. Both planar transcytosis initiated by Dynamin-mediated endocytosis and extracellular diffusion have been propo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.09.031
更新日期:2004-10-15 00:00:00
abstract::In this issue of Cell, Lee et al. (2006) report that the parasite Trypanosoma brucei synthesizes fatty acids in an unconventional way. T. brucei and two other trypanosomes use enzymes called elongases to synthesize myristate, a fourteen carbon (C14) fatty acid essential for pathogenesis. This is an unexpected finding ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2006.08.001
更新日期:2006-08-25 00:00:00
abstract::CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or parti...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.09.014
更新日期:2014-10-09 00:00:00
abstract::In this issue, Wilson et al. (2008) demonstrate that there are two functional subsets of hematopoietic stem cells that have distinctive kinetics of cell cycling. They present evidence that cells may transition between the two kinetic states, establishing one subpopulation that is ready to proliferate and another that ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.11.034
更新日期:2008-12-12 00:00:00
abstract::PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2016.02.065
更新日期:2016-03-24 00:00:00
abstract::Different species of fruit flies share habitats but are believed to mate with each other only rarely. In this issue, Fan et al. show that interspecies mating is inhibited by the taste receptor Gr32a (Gustatory receptor 32a) and a neural circuit in which it functions. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.06.015
更新日期:2013-07-03 00:00:00
abstract::Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.09.027
更新日期:2016-10-06 00:00:00
abstract::In extracts from rat and calf uterus, the steroid hormone 17 beta-estradiol stimulates the binding of its specific receptor protein to DNA. This interaction appears to be of low affinity (half of the estradiol-activated, 5S receptor bound at 300-400 mug/ml DNA) and nonspecific with respect to DNA base sequence. No bin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90150-6
更新日期:1975-04-01 00:00:00
abstract::We investigated soybean lectin gene structure and expression in a genetic line that accumulates normal levels of seed lectin (Le+ line) and in a mutant line that produces no detectable seed lectin (Le- line). We found that the nuclear and cytoplasmic lectin RNA levels in Le- embryos are approximately 0.01% of those in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90428-2
更新日期:1983-06-01 00:00:00
abstract::Uracil-DNA glycosylase inhibitor (Ugi) is a B. subtilis bacteriophage protein that protects the uracil-containing phage DNA by irreversibly inhibiting the key DNA repair enzyme uracil-DNA glycosylase (UDG). The 1.9 A crystal structure of Ugi complexed to human UDG reveals that the Ugi structure, consisting of a twiste...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90467-0
更新日期:1995-09-08 00:00:00
abstract::GAL4 derivatives containing an activation domain alleviated repression of a promoter during nucleosome assembly. A GAL4 derivative lacking an activation domain stably bound the promoter during nucleosome assembly but was not sufficient to preserve promoter function. The activation domain of GAL4 derivatives was essent...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90237-s
更新日期:1991-02-08 00:00:00
abstract::We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-inter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80024-6
更新日期:1985-06-01 00:00:00
abstract::We have isolated two adenylyl cyclase genes, designated ACA and ACG, from Dictyostelium. The proposed structure for ACA resembles that proposed for mammalian adenylyl cyclases: two large hydrophilic domains and two sets of six transmembrane spans. ACG has a novel structure, reminiscent of the membrane-bound guanylyl c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90411-5
更新日期:1992-04-17 00:00:00
abstract::Minimal hammerhead ribozymes have been characterized extensively by static and time-resolved crystallography as well as numerous biochemical analyses, leading to mutually contradictory mechanistic explanations for catalysis. We present the 2.2 A resolution crystal structure of a full-length Schistosoma mansoni hammerh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.06.036
更新日期:2006-07-28 00:00:00
abstract::Tyrosine phosphorylation controls many cellular functions. Yet the three-part toolkit that regulates phosphotyrosine signaling-tyrosine kinases, phosphotyrosine phosphatases, and Src Homology 2 (SH2) domains-is a relatively new innovation. Genomic analyses reveal how this revolutionary signaling system may have origin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.08.023
更新日期:2010-09-03 00:00:00
abstract::The ongoing pandemic of a new human coronavirus, SARS-CoV-2, has generated enormous global concern. We and others in China were involved in the initial genome sequencing of the virus. Herein, we describe what genomic data reveal about the emergence SARS-CoV-2 and discuss the gaps in our understanding of its origins. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.03.035
更新日期:2020-04-16 00:00:00
abstract::daughterless (da) has multiple functions in Drosophila embryonic development: maternal da activity is necessary for proper sex determination, and zygotic da activity is necessary for formation of the peripheral nervous system. We have cloned the region containing da and have found that five recessive lethal da mutatio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90250-4
更新日期:1988-12-23 00:00:00
abstract::Many body surfaces harbor organ-specific γδ T cell compartments that contribute to tissue integrity. Thus, murine dendritic epidermal T cells (DETCs) uniquely expressing T cell receptor (TCR)-Vγ5 chains protect from cutaneous carcinogens. The DETC repertoire is shaped by Skint1, a butyrophilin-like (Btnl) gene express...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.08.030
更新日期:2016-09-22 00:00:00
abstract::X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90379-9
更新日期:1984-06-01 00:00:00
abstract::The unfolded protein response (UPR) regulates gene expression in response to stress in the endoplasmic reticulum (ER). We determined the transcriptional scope of the UPR using DNA microarrays. Rather than regulating only ER-resident chaperones and phospholipid biosynthesis, as anticipated from earlier work, the UPR af...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80835-1
更新日期:2000-04-28 00:00:00
abstract::We here attempt to achieve an integrated understanding of the structure and dynamics of a number of higher-order assemblies, including amyloids, various kinds of signalosomes, and cellular granules. We propose that the synergy between folded domains, linear motifs, and intrinsically disordered regions regulates the fo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.05.004
更新日期:2016-05-19 00:00:00
abstract::Most bacteria that colonize eukaryotes must bind directly to host cells to establish a replicative niche. In enteric bacteria, adhesion to host cells is often promoted by a lectin found on surface-localized pili. Some pili promote efficient adhesion only when they are subjected to shear stress, as found during the flo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)00821-8
更新日期:2002-07-12 00:00:00
abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.063
更新日期:2012-02-17 00:00:00
abstract::A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90230-1
更新日期:1983-11-01 00:00:00
abstract::The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90710-5
更新日期:1986-09-26 00:00:00
abstract::The protein product of the human Duchenne muscular dystrophy locus (DMD) and its mouse homolog (mDMD) have been identified by using polyclonal antibodies directed against fusion proteins containing two distinct regions of the mDMD cDNA. The DMD protein is shown to be approximately 400 kd and to represent approximately...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90579-4
更新日期:1987-12-24 00:00:00
abstract::We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90295-7
更新日期:1983-04-01 00:00:00
abstract::Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.050
更新日期:2012-01-20 00:00:00
abstract::The Brachyury (T) gene is required for mesoderm formation in the mouse. In this paper we describe the cloning and expression of a Xenopus homolog of Brachyury, Xbra. As with Brachyury in the mouse, Xbra is expressed in presumptive mesodermal cells around the blastopore, and then in the notochord. We show that expressi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90573-h
更新日期:1991-10-04 00:00:00