Abstract：
:We have isolated two adenylyl cyclase genes, designated ACA and ACG, from Dictyostelium. The proposed structure for ACA resembles that proposed for mammalian adenylyl cyclases: two large hydrophilic domains and two sets of six transmembrane spans. ACG has a novel structure, reminiscent of the membrane-bound guanylyl cyclases. An aca- mutant, created by gene disruption, has little detectable adenylyl cyclase activity and fails to aggregate, demonstrating that cAMP is required for cell-cell communication. cAMP is not required for motility, chemotaxis, growth, and cell division, which are unaffected. Constitutive expression in aca- cells of either ACA or ACG, which is normally expressed only during germination, restores aggregation and the ability to complete the developmental program. ACA expression restores receptor and guanine nucleotide-regulated adenylyl cyclase activity, while activity in cells expressing ACG is insensitive to these regulators. Although they lack ACA, which has a transporter-like structure, the cells expressing ACG secrete cAMP constitutively.
journal_name
Celljournal_title
Cellauthors
Pitt GS,Milona N,Borleis J,Lin KC,Reed RR,Devreotes PNdoi
10.1016/0092-8674(92)90411-5subject
Has Abstractpub_date
1992-04-17 00:00:00pages
305-15issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(92)90411-5journal_volume
69pub_type
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