Abstract：
:Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight into how nucleosomes are arranged in vivo, we combined quantitative super-resolution nanoscopy with computer simulations to visualize and count nucleosomes along the chromatin fiber in single nuclei. Nucleosomes assembled in heterogeneous groups of varying sizes, here termed "clutches," and these were interspersed with nucleosome-depleted regions. The median number of nucleosomes inside clutches and their compaction defined as nucleosome density were cell-type-specific. Ground-state pluripotent stem cells had, on average, less dense clutches containing fewer nucleosomes and clutch size strongly correlated with the pluripotency potential of induced pluripotent stem cells. RNA polymerase II preferentially associated with the smallest clutches while linker histone H1 and heterochromatin were enriched in the largest ones. Our results reveal how the chromatin fiber is formed at nanoscale level and link chromatin fiber architecture to stem cell state.
journal_name
Celljournal_title
Cellauthors
Ricci MA,Manzo C,García-Parajo MF,Lakadamyali M,Cosma MPdoi
10.1016/j.cell.2015.01.054subject
Has Abstractpub_date
2015-03-12 00:00:00pages
1145-58issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(15)00132-4journal_volume
160pub_type
杂志文章相关文献
CELL文献大全abstract：:After translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in posttermination complexes (post-TCs), which must therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subunits. Recycling of bacterial post-TCs requires elongation factor EF-G ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.08.041
更新日期：2007-10-19 00:00:00
abstract：:Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae. Here, we present evidence that Ste5 forms a multikinase complex that joins these kinases for efficient Fus3 activation. B...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90427-8
更新日期：1994-08-12 00:00:00
abstract：:Tracing the lineage history of cells is key to answering diverse and fundamental questions in biology. Coupling of cell ancestry information with other molecular readouts represents an important goal in the field. Here, we describe the CRISPR array repair lineage tracing (CARLIN) mouse line and corresponding analysis ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.04.048
更新日期：2020-06-11 00:00:00
abstract：:Little is known about how neutrophils and other cells establish a single zone of actin assembly during migration. A widespread assumption is that the leading edge prevents formation of additional fronts by generating long-range diffusible inhibitors or by sequestering essential polarity components. We use morphologica...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.10.050
更新日期：2012-01-20 00:00:00
abstract：:The asymmetries of internal organs are consistently oriented along the left-right axis in all vertebrates, and perturbations of left-right orientation lead to significant congenital disease. We propose a model in which a "left-right coordinator" interacts with the Spemann organizer to coordinate the evolutionarily con...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81144-7
更新日期：1998-04-03 00:00:00
abstract：:Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90471-x
更新日期：1986-02-14 00:00:00
abstract：:We show that the high in vitro transcription efficiency of yeast RNA pol III is mainly due to rapid recycling. Kinetic analysis shows that RNA polymerase recycling on preassembled tDNA.TFIIIC.TFIIIB complexes is much faster than the initial transcription cycle. High efficiency of RNA pol III recycling is favored at hi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80979-4
更新日期：1996-01-26 00:00:00
abstract：:The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. We have identified a p53-inducible gene named TIGAR (TP53-induced glycolysis and apoptosis regulator). TIGAR expression lowered fruc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.05.036
更新日期：2006-07-14 00:00:00
abstract：:The general inhibition in transcriptional activity during mitosis abolishes the stress-inducible expression of the human hsp70 gene. Among the four transcription factors that bind to the human hsp70 promoter, the DNA-binding activities of three (C/EBP, GBP, and HSF1) were normal, while Sp1 showed reduced binding activ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90231-7
更新日期：1995-10-06 00:00:00
abstract：:Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90185-9
更新日期：1981-09-01 00:00:00
abstract：:To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.04.014
更新日期：2017-05-04 00:00:00
abstract：:Neuroinflammation is being increasingly recognized as a potential mediator of cognitive impairments in various neurological conditions. Habbas et al. demonstrate that the pro-inflammatory cytokine tumor necrosis factor alpha signals through astrocytes to alter synaptic transmission and impair cognition in a mouse mode...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.12.001
更新日期：2015-12-17 00:00:00
abstract：:Ocean microbial communities strongly influence the biogeochemistry, food webs, and climate of our planet. Despite recent advances in understanding their taxonomic and genomic compositions, little is known about how their transcriptomes vary globally. Here, we present a dataset of 187 metatranscriptomes and 370 metagen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.014
更新日期：2019-11-14 00:00:00
abstract：:In normal human fibroblasts, the synthesis of a cell surface receptor for plasma low density lipoprotein (LDL) is regulated by a sensitive system of feedback suppression. The number of functional LDL receptors declines by more than 20 fold when cellular stores of esterified cholesterol are increased by incubation of c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90110-0
更新日期：1976-10-01 00:00:00
abstract：:Small nucleolar RNAs represent an abundant, evolutionarily ancient group of noncoding RNAs which possess impressively diverse functions ranging from 2'-O-methylation and pseudouridylation of various classes of RNAs, through nucleolytic processing of rRNAs to the synthesis of telomeric DNA. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00718-3
更新日期：2002-04-19 00:00:00
abstract：:X-linked recessive chondrodysplasia punctata (CDPX) is a congenital defect of bone and cartilage development characterized by aberrant bone mineralization, severe underdevelopment of nasal cartilage, and distal phalangeal hypoplasia. A virtually identical phenotype is observed in the warfarin embryopathy, which is due...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90367-4
更新日期：1995-04-07 00:00:00
abstract：:Telomeres of Oxytricha nova macronuclear chromosomes consist of a repeated T4G4 sequence, single-stranded at the 3' terminus, bound by a heterodimeric protein. The cloning of genes for the two polypeptides and their separate expression in E. coli have enabled evaluation of their individual contributions to DNA binding...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90075-a
更新日期：1991-11-15 00:00:00
abstract：:Using integrative genomic analysis of 360 metastatic castration-resistant prostate cancer (mCRPC) samples, we identified a novel subtype of prostate cancer typified by biallelic loss of CDK12 that is mutually exclusive with tumors driven by DNA repair deficiency, ETS fusions, and SPOP mutations. CDK12 loss is enriched...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.04.034
更新日期：2018-06-14 00:00:00
abstract：:Two dimensional gel electrophoresis (triton-acid-urea followed by SDS) has been used to resolve two previously uncharacterized, quantitatively minor histone variants in acid extracts from macronuclei of Tetrahymena thermophila. Utilizing techniques which allow characterization of these variants without purifying them ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90307-4
更新日期：1980-07-01 00:00:00
abstract：:We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00700-6
更新日期：2002-04-19 00:00:00
abstract：:The presentation of peptides to T cells by MHC class II molecules is of critical importance in specific recognition by the immune system. Expression of class II molecules is exquisitely controlled at the transcriptional level. A large set of proteins interact with the promoters of class II genes. The most important of...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(02)00696-7
更新日期：2002-04-01 00:00:00
abstract：:Voluntary movement is a result of signals transmitted through a communication channel that links the internal world in our minds to the physical world around us. Intention can be considered the desire to effect change on our environment, and this is contained in the signals from the brain, passed through the nervous s...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.02.038
更新日期：2016-03-10 00:00:00
abstract：:All-trans retinoic acid (RA) has previously been shown to modulate the transcriptional properties of the retinoic acid receptor (RAR) and retinoid X receptor (RXR). The inability of all-trans RA to bind to RXR suggests that it may be metabolized to a more active high affinity ligand. We report here an experimental app...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90479-v
更新日期：1992-01-24 00:00:00
abstract：:We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics of 139 COVID-19 patients representing all levels of disease severity, from serial blood draws collected during the first week of infection following diagnosis. We identify a major shift between mild and moderate diseas...
journal_title：Cell
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.cell.2020.10.037
更新日期：2020-12-10 00:00:00
abstract：:During adaptive angiogenesis, a key process in the etiology and treatment of cancer and obesity, the vasculature changes to meet the metabolic needs of its target tissues. Although the cues governing vascular remodeling are not fully understood, target-derived signals are generally believed to underlie this process. H...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.12.008
更新日期：2014-01-16 00:00:00
abstract：:3.18 kb fragments of X. laevis DNA coding for tRNA 1 met have been inserted into a lambda vector via Hind III termini and cloned in E. coli. The organization of one cloned fragment has been analyzed by restriction endonuclease digestion and RNA-DNA hybridization. From the distribution of sites for three enzymes, this ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90253-2
更新日期：1978-07-01 00:00:00
abstract：:In this issue of Cell, a study by Valerie Mizrahi and her colleagues suggests that a putative error-prone DNA polymerase encoded by the dnaE2 gene of Mycobacterium tuberculosis may bypass certain types of DNA base damage, generating mutations. This may be an important mechanism for generating drug-resistant strains of...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(03)00275-7
更新日期：2003-04-18 00:00:00
abstract：:A Drosophila gene with similarity to the mammalian Ras GTPase activating protein has been isolated in screens for mutations that affect eye development. Inactivation of the locus, Gap1, mimics constitutive activation of the Sevenless receptor tyrosine kinase and eliminates the need for a functional Sevenless protein i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90073-l
更新日期：1992-03-20 00:00:00
abstract：:White adipose tissue (WAT) serves as the primary energy depot in the body by storing fat. During development, fat cell precursors (i.e., preadipocytes) undergo a hypertrophic response as they mature into lipid-laden adipocytes. However, the mechanisms that regulate adipocyte size and mass remain undefined. Herein, we ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.02.050
更新日期：2006-05-05 00:00:00
abstract：:Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2014.04.008
更新日期：2014-04-24 00:00:00
