Abstract:
:For the faithful initiation of transcription of exogenously added fibroin gene, a crude cellular extract was prepared from the posterior silk glands where fibroin gene is specifically transcribed. The faithful initiation was demonstrated by the assay with use of truncated fibroin gene templates and confirmed by nuclease S1 mapping. The transcription initiation of fibroin gene was detected also in the extract from the middle silk glands where fibroin gene is not transcribed, indicating that an expected suppression machinery for the gene was left behind during the extraction procedures. The promoter sequence of fibroin gene required in these homologous extracts (the nucleotide position -29 to +6) is the same as that in a heterologous HeLa cell extract. This result guarantees a limited usefulness of the HeLa cell extract in determining promotor sequence or analyzing general transcription machinery. In the homologous extracts, but not in the heterologous extract, however, it has been shown for the first time in vitro that the 5' flanking sequence upstream from -74 enhances the transcription initiation of fibroin gene. In addition, a remarkable fibroin gene preference in transcription over mouse beta globin and adenovirus 2 major late genes was observed in the homologous extracts. The fibroin gene preference is partly caused by the sequence element upstream from -74 of fibroin DNA.
journal_name
Celljournal_title
Cellauthors
Tsuda M,Suzuki Ydoi
10.1016/0092-8674(81)90371-8subject
Has Abstractpub_date
1981-11-01 00:00:00pages
175-82issue
1 Pt 2eissn
0092-8674issn
1097-4172pii
0092-8674(81)90371-8journal_volume
27pub_type
杂志文章相关文献
CELL文献大全abstract::Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(88)90977-4
更新日期:1988-09-09 00:00:00
abstract::Several recent papers show that differences in histone modification and the use of histone variants at the 5' and 3' ends of genes influence the location and kinetics of transcriptional initiation. The ultimate target of most epigenetic mechanisms may be the regulation of nucleosome occupancy, which in turn controls a...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.12.010
更新日期:2005-12-29 00:00:00
abstract::Notch signaling regulates many aspects of metazoan development and tissue renewal. Accordingly, the misregulation or loss of Notch signaling underlies a wide range of human disorders, from developmental syndromes to adult-onset diseases and cancer. Notch signaling is remarkably robust in most tissues even though each ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2009.03.045
更新日期:2009-04-17 00:00:00
abstract::Dot1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, Dot1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3. We have solved a 2.5 A resolution structure of the catalytic doma...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00114-4
更新日期:2003-03-07 00:00:00
abstract::Sleep is characterized by behavioral quiescence, homeostasis, increased arousal threshold, and rapid reversibility. Understanding how these properties are encoded by a neuronal circuit has been difficult, and no single molecular or neuronal pathway has been shown to be responsible for the regulation of sleep. Taking a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.11.036
更新日期:2014-01-16 00:00:00
abstract::Hematopoietic stem cells (HSCs) develop from a specialized subpopulation of endothelial cells known as hemogenic endothelium (HE). Although the HE origin of HSCs is now well established in different species, the signaling pathways that control this transition remain poorly understood. Here, we show that activation of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.08.055
更新日期:2013-09-26 00:00:00
abstract::Temperature-sensitive mutants of vesicular stomatitis virus (VSV) belonging to complementation group III contain a lesion in the matrix (M) protein. This results in a 2--5 fold increase in transcription at the nonpermissive temperature. Co-infection of cells with one of these mutants and wild-type virus reverses this ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90069-7
更新日期:1978-12-01 00:00:00
abstract::In Drosophila, flies with two X chromosomes are females, with one X chromosome, males. We investigated the presence of sex determining factors on the X chromosome by constructing genotypes with one X and various X-chromosomal duplications. We found that female determining factors are not evenly distributed along the X...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90284-3
更新日期:1985-10-01 00:00:00
abstract::Brassinosteroids (BRs) signal through a plasma membrane-localized receptor kinase to regulate plant growth and development. We showed previously that a novel protein, BES1, accumulates in the nucleus in response to BRs, where it plays a role in BR-regulated gene expression; however, the mechanism by which BES1 regulat...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.044
更新日期:2005-01-28 00:00:00
abstract::Synonymous mutations change the sequence of a gene without directly altering the sequence of the encoded protein. Here, we present evidence that these "silent" mutations frequently contribute to human cancer. Selection on synonymous mutations in oncogenes is cancer-type specific, and although the functional consequenc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.01.051
更新日期:2014-03-13 00:00:00
abstract::We have shown that in fixed mitotic chromosomes from female G. gerbillus cells the inactive X chromosome is distinctly less sensitive to DNAase I than the active X chromosome, as demonstrated by in situ nick translation. These results indicated that the specific chromatin conformation that renders potentially active g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90504-x
更新日期:1984-09-01 00:00:00
abstract::Understanding molecular mechanisms that dictate B cell diversity is important for targeting B cells as anti-cancer treatment. Through the single-cell dissection of B cell heterogeneity in longitudinal samples of patients with breast cancer before and after neoadjuvant chemotherapy, we revealed that an ICOSL+ B cell su...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.02.015
更新日期:2020-03-19 00:00:00
abstract::Here, we show that the beta-chemokine receptor CKR-5 serves as a cofactor for M-tropic HIV viruses. Expression of CKR-5 with CD4 enables nonpermissive cells to form syncytia with cells expressing M-tropic, but not T-tropic, HIV-1 env proteins. Expression of CKR-5 and CD4 enables entry of a M-tropic, but not a T-tropic...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81314-8
更新日期:1996-06-28 00:00:00
abstract::A second and minor DNA component containing 5s RNA genes has been purified from the genomic DNA of Xenopus laevis (XIt 5S DNA). Some of its physical and chemical characteristics are described. It contains a 5S RNA gene sequence which has some oocyte and some somatic-specific residues, as well as nucleotides which diff...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90168-4
更新日期:1977-12-01 00:00:00
abstract::We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81933-9
更新日期:1997-03-21 00:00:00
abstract::The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81732-8
更新日期:1998-09-18 00:00:00
abstract::The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00191-0
更新日期:2003-03-21 00:00:00
abstract::The crystal structure of the snake toxin fasciculin, bound to mouse acetylcholinesterase (mAChE), at 3.2 A resolution reveals a synergistic three-point anchorage consistent with the picomolar dissociation constant of the complex. Loop II of fasciculin contains a cluster of hydrophobic residues that interact with the p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90128-0
更新日期:1995-11-03 00:00:00
abstract::N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.05.014
更新日期:2015-06-04 00:00:00
abstract::Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated CaMKII phenocopied structural abnormalities of dlg mut...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81964-9
更新日期:1999-08-06 00:00:00
abstract::Cancer cells enter a reversible drug-tolerant persister (DTP) state to evade death from chemotherapy and targeted agents. It is increasingly appreciated that DTPs are important drivers of therapy failure and tumor relapse. We combined cellular barcoding and mathematical modeling in patient-derived colorectal cancer mo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.11.018
更新日期:2021-01-07 00:00:00
abstract::Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.10.008
更新日期:2015-11-05 00:00:00
abstract::The phage Mu transpososome is assembled by interactions of transposase subunits with the left (L) and right (R) ends of Mu and an enhancer (E) located in between. A metastable three-site complex LER progresses into a more stable type 0 complex in which a tetrameric transposase is poised for DNA cleavage. "Difference t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00728-6
更新日期:2002-05-17 00:00:00
abstract::We report genome-wide DNA data for 73 individuals from five archaeological sites across the Bronze and Iron Ages Southern Levant. These individuals, who share the "Canaanite" material culture, can be modeled as descending from two sources: (1) earlier local Neolithic populations and (2) populations related to the Chal...
journal_title:Cell
pub_type: 历史文章,杂志文章
doi:10.1016/j.cell.2020.04.024
更新日期:2020-05-28 00:00:00
abstract::Different species of fruit flies share habitats but are believed to mate with each other only rarely. In this issue, Fan et al. show that interspecies mating is inhibited by the taste receptor Gr32a (Gustatory receptor 32a) and a neural circuit in which it functions. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2013.06.015
更新日期:2013-07-03 00:00:00
abstract::During Tetrahymena conjugation, programmed DNA degradation occurs in two separate nuclei. Thousands of germline-specific deletion elements are removed from the genome of the developing somatic macronucleus, and the old parental macronucleus is degraded by an apoptotic mechanism. An abundant polypeptide, Pdd1p (formerl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81324-0
更新日期:1996-10-04 00:00:00
abstract::torso encodes a receptor tyrosine kinase (torso) required for anterior and posterior terminal development of the Drosophila embryo. Injecting eggs with in vitro synthesized torso mRNAs revealed that torso activation is governed by an extracellular molecule, presumably the torso ligand, produced at terminal regions of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90394-r
更新日期:1992-12-11 00:00:00
abstract::Behavioral expression of food-associated memory in fruit flies is constrained by satiety and promoted by hunger, suggesting an influence of motivational state. Here, we identify a neural mechanism that integrates the internal state of hunger and appetitive memory. We show that stimulation of neurons that express neuro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.08.035
更新日期:2009-10-16 00:00:00
abstract::Who are science journalists, and how can journalists and research scientists work together to improve science communication? ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.10.051
更新日期:2016-11-17 00:00:00
abstract::We have identified in mice an allele of a new T cell receptor V beta gene, V beta 17a, whose product is bound by the monoclonal antibody KJ23a. Over 90% of T cell hybridomas prepared from V beta 17a+ T cells of SWR mice respond to allogeneic forms of the IE class II MHC protein, indicating that V beta 17a has an appre...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90567-8
更新日期:1987-04-24 00:00:00