Abstract:
:We have shown that in fixed mitotic chromosomes from female G. gerbillus cells the inactive X chromosome is distinctly less sensitive to DNAase I than the active X chromosome, as demonstrated by in situ nick translation. These results indicated that the specific chromatin conformation that renders potentially active genes sensitive to DNAase I is maintained in fixed mitotic chromosomes. We increased the sensitivity and accuracy of in situ nick translation using biotinylated dUTP and a specific detection and staining procedure instead of radioactive label and autoradiography and now show that in both human and CHO chromosomes, the DNAase I sensitive and insensitive chromosomal regions form a specific dark and light banding pattern. The DNAase I sensitive dark D-bands usually correspond to the light G-bands, but not all light G-bands are DNAase I sensitive. Identifiable regions of inactive constitutive heterochromatin are in a DNAase I insensitive conformation. Our methodology provides a new and important tool for studying the structural and functional organization of chromosomes.
journal_name
Celljournal_title
Cellauthors
Kerem BS,Goitein R,Diamond G,Cedar H,Marcus Mdoi
10.1016/0092-8674(84)90504-xsubject
Has Abstractpub_date
1984-09-01 00:00:00pages
493-9issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(84)90504-Xjournal_volume
38pub_type
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