Abstract：
:Chromatin modifying activities inherent to polycomb repressive complexes PRC1 and PRC2 play an essential role in gene regulation, cellular differentiation, and development. However, the mechanisms by which these complexes recognize their target sites and function together to form repressive chromatin domains remain poorly understood. Recruitment of PRC1 to target sites has been proposed to occur through a hierarchical process, dependent on prior nucleation of PRC2 and placement of H3K27me3. Here, using a de novo targeting assay in mouse embryonic stem cells we unexpectedly discover that PRC1-dependent H2AK119ub1 leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain. This activity is restricted to variant PRC1 complexes, and genetic ablation experiments reveal that targeting of the variant PCGF1/PRC1 complex by KDM2B to CpG islands is required for normal polycomb domain formation and mouse development. These observations provide a surprising PRC1-dependent logic for PRC2 occupancy at target sites in vivo.
journal_name
Celljournal_title
Cellauthors
Blackledge NP,Farcas AM,Kondo T,King HW,McGouran JF,Hanssen LLP,Ito S,Cooper S,Kondo K,Koseki Y,Ishikura T,Long HK,Sheahan TW,Brockdorff N,Kessler BM,Koseki H,Klose RJdoi
10.1016/j.cell.2014.05.004subject
Has Abstractpub_date
2014-06-05 00:00:00pages
1445-1459issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(14)00589-3journal_volume
157pub_type
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