Abstract:
:We have isolated and characterized several members of the P transposable element family from a Drosophila melanogaster P strain. Large 2.9 kb elements are present as multiple highly conserved copies together with smaller (0.5-1.6 kb), heterogeneous elements. The complete DNA sequences of the 2.9 kb element and four small elements (previously isolated from hybrid-dysgenesis-induced mutations of the white locus) have been determined. Each small element appears to have arisen from the 2.9 kb element by a different internal deletion. P elements have 31 bp perfect inverse terminal repeats and upon insertion duplicate an 8 bp sequence found only once at the site of insertion. Three of the insertions into the white locus occurred at the same nucleotide, indicating a high degree of local site specificity for insertion. The basis of this specificity has been investigated by DNA sequence analysis of the sites where 18 P elements are found. A revertant of one of the white locus mutants has been found to result from precise excision of the P element, restoring the wild-type DNA sequence.
journal_name
Celljournal_title
Cellauthors
O'Hare K,Rubin GMdoi
10.1016/0092-8674(83)90133-2subject
Has Abstractpub_date
1983-08-01 00:00:00pages
25-35issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(83)90133-2journal_volume
34pub_type
杂志文章相关文献
CELL文献大全abstract::The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may have an active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptor-like functio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00191-x
更新日期:2001-01-12 00:00:00
abstract::Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies, but their subclass composition changed over time, with IgG1 persisting, IgG3 r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.036
更新日期:2019-05-30 00:00:00
abstract::We present evidence that the genes encoding U3 snRNA in plants are transcribed by RNA polymerase III (pol III) and not by RNA polymerase II (pol II) as in vertebrates or lower eukaryotes. The U3 gene is the only known example of a gene transcribed by different polymerases in different organisms. It is possible to conv...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90469-f
更新日期:1991-05-03 00:00:00
abstract::Elucidation of endogenous cellular protein-protein interactions and their networks is most desirable for biological studies. Here we report our study of endogenous human coregulator protein complex networks obtained from integrative mass spectrometry-based analysis of 3290 affinity purifications. By preserving weak pr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.05.006
更新日期:2011-05-27 00:00:00
abstract::The mammalian circadian timing system is composed of a central pacemaker in the suprachiasmatic nucleus (SCN) of the brain and subsidiary oscillators in most peripheral cell types. While oscillators in SCN neurons are known to function in a self-sustained fashion, peripheral oscillators have been thought to damp rapid...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.015
更新日期:2004-11-24 00:00:00
abstract::Eukaryotic translation initiation requires coordinated assembly of a remarkable array of initiation factors onto the small ribosomal subunit to select an appropriate mRNA start codon. Studies from Erzberger et al. and Hussain et al. bring new insights into this mechanism by looking at early and late initiation interme...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.10.005
更新日期:2014-10-23 00:00:00
abstract::In a screen for genes that interact with the Rap1 GTPase, we have identified a Drosophila gene, dacapo (dap), which is a member of the p21/p27 family of cdk inhibitors. Unlike mammalian cdk inhibitors studied to date, dap is essential for normal embryonic development. Dacapo inhibits cyclin-cdk activity in vitro. Over...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81819-x
更新日期:1996-12-27 00:00:00
abstract::Upon exposure to 0.1 mM N-phosphonacetyl-L-aspartate (PALA), a transition state analog inhibitor of aspartate transcarbamylase, most cells of a simian virus 40 (SV40)-transformed Syrian hamster line are killed within a few days, but resistant mutants form spontaneously with frequency 2-5 X 10(-5) in a stochastic proce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90036-2
更新日期:1976-12-01 00:00:00
abstract::Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of unde...
journal_title:Cell
pub_type: 历史文章,杂志文章
doi:10.1016/j.cell.2012.01.002
更新日期:2012-01-20 00:00:00
abstract::The origin recognition complex (ORC) is required to initiate eukaryotic DNA replication and also engages in transcriptional silencing in S. cerevisiae. We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes. HP1, a heterochromatin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80415-8
更新日期:1997-10-31 00:00:00
abstract::Ventral midline cells at different rostrocaudal levels of the central nervous system exhibit distinct properties but share the ability to pattern the dorsoventral axis of the neural tube. We show here that ventral midline cells acquire distinct identities in response to the different signaling activities of underlying...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80334-7
更新日期:1997-07-25 00:00:00
abstract::Most secreted growth factors and cytokines are functionally pleiotropic because their receptors are expressed on diverse cell types. While important for normal mammalian physiology, pleiotropy limits the efficacy of cytokines and growth factors as therapeutics. Stem cell factor (SCF) is a growth factor that acts throu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.02.011
更新日期:2017-03-09 00:00:00
abstract::Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90440-2
更新日期:1981-03-01 00:00:00
abstract::Steroid hormones induce transcription from the mouse mammary tumor virus (MMTV) promoter by complex mechanisms requiring binding of the hormone receptors to the hormone responsive element (HRE) of the long terminal repeat region. Here we show that the MMTV promoter contains two degenerated octamer motifs immediately u...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90240-y
更新日期:1991-02-08 00:00:00
abstract::Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and lab...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.064
更新日期:2016-06-30 00:00:00
abstract::The formation of morphogen gradients is essential for tissue patterning. Morphogens are released from producing cells and spread through adjacent tissue; paradoxically, however, many morphogens, including Wingless, associate tightly with the cell membrane. Here, we describe a novel cell biological mechanism that dispe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00484-6
更新日期:2001-09-07 00:00:00
abstract::Many body surfaces harbor organ-specific γδ T cell compartments that contribute to tissue integrity. Thus, murine dendritic epidermal T cells (DETCs) uniquely expressing T cell receptor (TCR)-Vγ5 chains protect from cutaneous carcinogens. The DETC repertoire is shaped by Skint1, a butyrophilin-like (Btnl) gene express...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.08.030
更新日期:2016-09-22 00:00:00
abstract::The amino acid glutamine has a central role in nitrogen metabolism. Although the molecular mechanisms responsible for its transport across cell membranes remain poorly understood, classical amino acid transport system N appears particularly important. Using intracellular pH measurements, we have now identified an orph...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81674-8
更新日期:1999-12-23 00:00:00
abstract::As axons grow past intermediate targets, they change their responsiveness to guidance cues. Local upregulation of receptor expression is involved, but the mechanisms for this are not clear. Here protein synthesis is traced within individual axons by introducing RNAs encoding visualizable reporters. Individual severed ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00813-9
更新日期:2002-07-26 00:00:00
abstract::Two studies from Jarosz et al. describe how [GAR(+)], a protein-based epigenetic determinant found mainly in wild yeast strains, can be activated by microbial cross-kingdom communication. With the aid of genetically and ecologically diverse bacteria, yeast can override an ancient regulatory mechanism of glucose repres...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.08.004
更新日期:2014-08-28 00:00:00
abstract::Given the heterogeneity of senescent cells, our knowledge of both the drivers and consequences of cellular senescence in tissues and organs remains limited, as is our understanding of how this process could be harnessed for human health. Here we identified five broad areas that would help propel the field forward. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.10.032
更新日期:2020-11-25 00:00:00
abstract::Malignancy can be suppressed by the immune system in a process termed immunosurveillance. However, to what extent immunosurveillance occurs in spontaneous cancers and the composition of participating cell types remains obscure. Here, we show that cell transformation triggers a tissue-resident lymphocyte response in on...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.01.002
更新日期:2016-01-28 00:00:00
abstract::The CD4 lymphocyte surface glycoprotein and the lck tyrosine protein kinase p56lck are found as a complex in T lymphocytes. We have defined the domains in both proteins that are responsible for this interaction by coexpressing hybrid and deleted forms of the two proteins in HeLa cells. We have found that the unique 32...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90008-1
更新日期:1989-11-17 00:00:00
abstract::Animal cells can change shape and move by using actin polymerization to drive plasma membrane protrusion. Giannone and coworkers (this issue of Cell) describe how cells periodically pull back on these protrusions in order to sense and respond to the rigidity of their environment. ...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s0092-8674(04)00123-0
更新日期:2004-02-06 00:00:00
abstract::We have characterized regulation of a complex Chlamydomonas reinhardtii chloroplast (PA) whose activity is stimulated by the DNA gyrase inhibitor novobiocin, both in the alga itself and in a heterologous Escherichia coli plasmid system. Since novobiocin is known to reduce torsional stress in E. coli DNA, we interpret ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90431-4
更新日期:1987-01-30 00:00:00
abstract::Vascular endothelial growth factor (VEGF), a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. We now describe the purification and the expression cloning from tumor cells of a third VEGF receptor, one that binds VEGF165 but not VEGF121. This isoform-specific VEGF receptor (...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81402-6
更新日期:1998-03-20 00:00:00
abstract::p53 is a key tumor suppressor protein that has numerous functions. Its primary mode of action has generally been ascribed to the induction of cell-cycle arrest, apoptosis, or senescence upon stress. Li et al. challenge this dogma with evidence that all three of these programs are dispensable for p53's tumor suppressiv...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.05.024
更新日期:2012-06-08 00:00:00
abstract::Using insertional mutagenesis, we have isolated a "stalky" mutant in which cells destined to become spores end up as stalk cells. Similar mutants were previously observed after chemical mutagenesis, but the affected gene could not be isolated. Our mutant, like the previous ones, is in stkA. Its defect is cell-autonomo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81367-7
更新日期:1996-11-01 00:00:00
abstract::The molecular nature of mutations arising in dysgenic hybrids between P and M Drosophila melanogaster strains has been investigated. Seven independent mutations at the white locus were examined, and these fell into two classes on the basis of their genetic and structural properties. The five mutations comprising the f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90462-7
更新日期:1982-07-01 00:00:00
abstract::Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90222-7
更新日期:1977-02-01 00:00:00