Abstract:
:The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA binding specificity but instead through the use of promoter-selective activation domains. These distinct activation domains lead to stimulation of the U2 small nuclear RNA promoter by Oct-1 and an mRNA promoter by Oct-2. An Oct-2 variant, called Oct-2B, differs from Oct-2 by an Oct-1-related C-terminal extension that results from alternative splicing. This variant gains the ability to activate the U2 small nuclear RNA promoter. Thus, the promoter selectivity of a transcriptional activator can be changed, in this case by alternative splicing, without affecting its DNA binding specificity.
journal_name
Celljournal_title
Cellauthors
Tanaka M,Lai JS,Herr Wdoi
10.1016/0092-8674(92)90150-bsubject
Has Abstractpub_date
1992-02-21 00:00:00pages
755-67issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(92)90150-Bjournal_volume
68pub_type
杂志文章相关文献
CELL文献大全abstract::Drugs selectively targeting CB2 hold promise for treating neurodegenerative disorders, inflammation, and pain while avoiding psychotropic side effects mediated by CB1. The mechanisms underlying CB2 activation and signaling are poorly understood but critical for drug design. Here we report the cryo-EM structure of the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.01.007
更新日期:2020-02-20 00:00:00
abstract::The mouse kreisler (kr) mutation causes segmentation abnormalities in the caudal hindbrain and defective inner ear development. Based on an inversion discovered in the original kr allele, we selected a candidate cDNA highly expressed in the developing caudal hindbrain. This cDNA encodes a basic domain-leucine zipper (...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90033-7
更新日期:1994-12-16 00:00:00
abstract::How and when memory T cells form during an immune response are long-standing questions. To better understand memory CD8 T cell development, a time course of gene expression and functional changes in antigen-specific T cells during viral infection was evaluated. The expression of many genes continued to change after vi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01139-x
更新日期:2002-12-13 00:00:00
abstract::The rps12 gene codes for chloroplast ribosomal protein S12. In the tobacco chloroplast genome (156 kbp circular DNA), exons II and III of this gene are separated by an intron of 536 bases and are present in two copies in the inverted repeat region, while exon I is located in the large single copy region at a distance ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90361-8
更新日期:1987-01-16 00:00:00
abstract::Replicative intermediates of SV40 were isolated, digested with the restriction endonuclease Bgl I and examined by electron microscopy. Over 98% of the replicative intermediates isolated following infection with wild-type virions at 33 degrees, 37 degrees or 40 degrees C or with tsA209 at 33 degrees C had initiated rep...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90624-8
更新日期:1980-06-01 00:00:00
abstract::Observational learning is a powerful survival tool allowing individuals to learn about threat-predictive stimuli without directly experiencing the pairing of the predictive cue and punishment. This ability has been linked to the anterior cingulate cortex (ACC) and the basolateral amygdala (BLA). To investigate how inf...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.04.004
更新日期:2018-05-31 00:00:00
abstract::To determine the special feature of ribosomal RNA promoters that might account for the highly efficient and regulated synthesis of rRNA in E. coli, we have analyzed the beginnings of two ribosomal RNA operons, rrnD and rrnX. DNA sequences for 425 bp preceding those specifying mature 16s rRNA are reported. In vitro tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90310-6
更新日期:1979-05-01 00:00:00
abstract::The protein product of the human Duchenne muscular dystrophy locus (DMD) and its mouse homolog (mDMD) have been identified by using polyclonal antibodies directed against fusion proteins containing two distinct regions of the mDMD cDNA. The DMD protein is shown to be approximately 400 kd and to represent approximately...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90579-4
更新日期:1987-12-24 00:00:00
abstract::To investigate the function of SWI/SNF in site-specific chromatin remodeling at promoters, we have used a purified system to analyze its distribution, function, and retention following recruitment by a sequence-specific transcription activator. Activator recruitment of SWI/SNF bound the complex to promoter proximal nu...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00279-3
更新日期:2001-03-23 00:00:00
abstract::Endoplasmic reticulum (ER) membrane contact sites (MCSs) mark positions where endosomes undergo fission for cargo sorting. To define the role of ER at this unique MCS, we targeted a promiscuous biotin ligase to cargo-sorting domains on endosome buds. This strategy identified the ER membrane protein TMCC1, a member of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.08.030
更新日期:2018-09-20 00:00:00
abstract::The looming threat of a new influenza virus pandemic has fueled ambitious efforts to devise more predictive parameters for assessing the risks associated with emergent virus strains. At the same time, a comprehensive understanding of critical factors that can accurately predict the outcome of vaccination is sorely nee...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.023
更新日期:2014-04-10 00:00:00
abstract::Morphogen gradients control body pattern by differentially regulating cellular behavior. Here, we analyze the molecular events underlying the primary response to the Dpp/BMP morphogen in Drosophila. Throughout development, Dpp transduction causes the graded transcriptional downregulation of the brinker (brk) gene. We ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00241-1
更新日期:2003-04-18 00:00:00
abstract::The somatic 5S DNA from X. borealis (Xbs 5S DNA) and X. laevis (Xis 5S DNA) and a minor oocyte-specific 5S DNA from X. laevis (Xit 5S DNA) have been purified, and individual repeating units have been cloned and sequenced. The two somatic 5S DNAs differ from the major oocyte 5S DNAs in having GC-rich spacers, homogeneo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90241-x
更新日期:1980-05-01 00:00:00
abstract::The molybdenum-containing enzyme sulfite oxidase catalyzes the conversion of sulfite to sulfate, the terminal step in the oxidative degradation of cysteine and methionine. Deficiency of this enzyme in humans usually leads to major neurological abnormalities and early death. The crystal structure of chicken liver sulfi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80488-2
更新日期:1997-12-26 00:00:00
abstract::TGF-beta signaling controls a plethora of cellular responses and figures prominently in animal development. Recent cellular, biochemical, and structural studies have revealed significant insight into the mechanisms of the activation of TGF-beta receptors through ligand binding, the activation of Smad proteins through ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(03)00432-x
更新日期:2003-06-13 00:00:00
abstract::We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics of 139 COVID-19 patients representing all levels of disease severity, from serial blood draws collected during the first week of infection following diagnosis. We identify a major shift between mild and moderate diseas...
journal_title:Cell
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.cell.2020.10.037
更新日期:2020-12-10 00:00:00
abstract::We show that expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme. Expression is reduced at least 200-fold in cells grown under conditions of heme deficiency. Studies on the regulation of a CYC1-lacZ fused gene and direct determination of mRN...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90309-4
更新日期:1983-04-01 00:00:00
abstract::We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90225-3
更新日期:1984-02-01 00:00:00
abstract::Smad4 is an important mediator of signaling by TGFbeta family members that is presumed to be controlled by the phosphorylation status of its partners, the receptor-regulated R-Smads. In this issue, Dupont et al. (2009) now reveal that Smad4 itself is subject to cycles of ubiquitination and deubiquitination that regula...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.12.028
更新日期:2009-01-09 00:00:00
abstract::Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81615-3
更新日期:1998-11-13 00:00:00
abstract::A clonally diverse anticipatory repertoire in which each lymphocyte bears a unique antigen receptor is the central feature of the adaptive immune system that evolved in our vertebrate ancestors. The survival advantage gained through adding this type of adaptive immune system to a pre-existing innate immune system led ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2006.02.001
更新日期:2006-02-24 00:00:00
abstract::Despite tremendous progress made toward the identification of the molecular circuitry that governs cell fate in embryonic stem cells, genes controlling this process in the adult hematopoietic stem cell have proven to be more difficult to unmask. We now report the results of a novel gain-of-function screening approach,...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.03.026
更新日期:2009-04-17 00:00:00
abstract::Antigen presenting cells (macrophages and dendritic cells) express pattern recognition molecules that are thought to recognize foreign ligands during early phases of the immune response. The best known of these are probably the Toll-like receptors, but a number of other receptors are also involved. Several of these re...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(02)01201-1
更新日期:2002-12-27 00:00:00
abstract::Balancing the capacity for protein maturation with changes in protein flux through the endoplasmic reticulum (ER) is crucial for maintaining ER homeostasis. In this issue, Merksamer et al. (2008) exploit a redox-sensitive fluorescent protein to monitor the environment inside the ER of living yeast, illuminating how th...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.11.004
更新日期:2008-11-28 00:00:00
abstract::The pathway of RNA polymerase entry at the lac promoter was studied by investigating the relationship between the promoter and a weak, overlapping polymerase interaction site (P2). If polymerase is made to enter the DNA by binding in vitro at this P2 site, cyclic AMP receptor protein (CRP) actively removes polymerase ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90250-8
更新日期:1985-12-01 00:00:00
abstract::We present genetic evidence that integrins regulate epithelial-mesenchymal interactions during organogenesis. Mice with a mutation in the alpha8 gene do not express the integrin alpha8 beta1 and exhibit profound deficits in kidney morphogenesis. In wild-type animals, inductive interactions between the ureteric epithel...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81903-0
更新日期:1997-03-07 00:00:00
abstract::Complex multicellular organisms, such as mammals, express two complete sets of chromosomes per nucleus, combining the genetic material of both parents. However, epigenetic studies have demonstrated violations to this rule that are necessary for mammalian physiology; the most notable parental allele expression phenomen...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.01.043
更新日期:2019-02-21 00:00:00
abstract::Methylation of arginine residues within histone H3 has been linked to active transcription. This modification appears on the estrogen-regulated pS2 promoter when the CARM1 methyltransferase is recruited during transcriptional activation. Here we describe a process, deimination, that converts histone arginine to citrul...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.08.020
更新日期:2004-09-03 00:00:00
abstract::STAT6 plays a prominent role in adaptive immunity by transducing signals from extracellular cytokines. We now show that STAT6 is required for innate immune signaling in response to virus infection. Viruses or cytoplasmic nucleic acids trigger STING (also named MITA/ERIS) to recruit STAT6 to the endoplasmic reticulum, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.09.022
更新日期:2011-10-14 00:00:00
abstract::Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted P...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.05.028
更新日期:2017-06-01 00:00:00