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The isolation and characterization of a second oocyte 5s DNA from Xenopus laevis.

Abstract：

:A second and minor DNA component containing 5s RNA genes has been purified from the genomic DNA of Xenopus laevis (XIt 5S DNA). Some of its physical and chemical characteristics are described. It contains a 5S RNA gene sequence which has some oocyte and some somatic-specific residues, as well as nucleotides which differ from both types of 5S RNA. There are about 2000 of these 5S RNA genes per haploid complement of DNA compared to about 24,000 of the principal oocyte 5S RNA genes. The multiple repeating units of XIt 5S DNA are homogeneous in length (about 350 base pairs). We present evidence that XIt 5S RNA is transcribed in ovaries but not in somatic cells; XIt 5S DNA may therefore be under the same control as the major oocyte 5S DNA.

journal_name

Cell

journal_title

Cell

authors

Brown DD,Carrol D,Brown RD

doi

10.1016/0092-8674(77)90168-4

subject

Has Abstract

pub_date

1977-12-01 00:00:00

pages

1045-56

issue

4

eissn

0092-8674

issn

1097-4172

pii

0092-8674(77)90168-4

journal_volume

12

pub_type

杂志文章

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