Abstract：
:We have reconstituted the fusion and assembly of vesiculated Golgi membranes (VGMs) into functionally active stacks of cisternae. A kinetic analysis of this assembly process revealed that highly dispersed VGMs of 60-90 nm diameter first fuse to form larger vesicles of 200-300 nm diameter that are clustered together. These vesicles then fuse to form tubular elements and short cisternae, which finally assemble into stacks of cisternae. We now provide evidence that the sequential stack formation from VGMs reflects two distinct fusion processes: the first event is N-ethyl-maleimide (NEM)-sensitive factor (NSF) dependent, and the second fusion event requires an NSF-like NEM-sensitive ATPase called p97. Interestingly, while the earliest steps in stack formation share some similarities with events catalyzing fusion of transport vesicles to its target membrane, neither GTP gamma S nor Rab-GDI, inhibitors of vesicular protein traffic, inhibit stack formation.
journal_name
Celljournal_title
Cellauthors
Acharya U,Jacobs R,Peters JM,Watson N,Farquhar MG,Malhotra Vdoi
10.1016/0092-8674(95)90269-4subject
Has Abstractpub_date
1995-09-22 00:00:00pages
895-904issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(95)90269-4journal_volume
82pub_type
杂志文章相关文献
CELL文献大全abstract：:We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81164-2
更新日期：1998-05-01 00:00:00
abstract：:Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.10.011
更新日期：2016-11-03 00:00:00
abstract：:Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipop...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.06.059
更新日期：2015-07-30 00:00:00
abstract：:The mouse kreisler (kr) mutation causes segmentation abnormalities in the caudal hindbrain and defective inner ear development. Based on an inversion discovered in the original kr allele, we selected a candidate cDNA highly expressed in the developing caudal hindbrain. This cDNA encodes a basic domain-leucine zipper (...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90033-7
更新日期：1994-12-16 00:00:00
abstract：:Cells are devices whose structures delimit function. For example, in the nervous system, neuronal and glial shapes dictate paths of information flow. To understand how cells acquire their shapes, we examined the formation of a sense organ in C. elegans. Using time-lapse imaging, we found that sensory dendrites form by...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.01.057
更新日期：2009-04-17 00:00:00
abstract：:The chronic autoimmune diseases include multiple complex genetic disorders. Recently, genome-wide association studies (GWAS) have identified a large number of major loci, with many associations shared between various autoimmune diseases. These associations highlight key roles for lymphocyte activation and prioritize s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.03.003
更新日期：2010-03-19 00:00:00
abstract：:During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dyna...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.11.002
更新日期：2011-11-23 00:00:00
abstract：:Apoptosis induced by TNF-receptor I (TNFR1) is thought to proceed via recruitment of the adaptor FADD and caspase-8 to the receptor complex. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. The mechanism by which this decision between cell death and survival is arbitr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00521-x
更新日期：2003-07-25 00:00:00
abstract：:We have isolated and characterized a set of overlapping cDNA clones that encode the human centromere autoantigen centromere protein C (CENP-C). The identity of these clones has been established using several criteria. First, they were shown to encode a polypeptide that migrates at the expected position for CENP-C on S...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90538-n
更新日期：1992-07-10 00:00:00
abstract：:beta-glucocerebrosidase, the enzyme defective in Gaucher disease, is targeted to the lysosome independently of the mannose-6-phosphate receptor. Affinity-chromatography experiments revealed that the lysosomal integral membrane protein LIMP-2 is a specific binding partner of beta-glucocerebrosidase. This interaction in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.10.018
更新日期：2007-11-16 00:00:00
abstract：:T lymphocyte selection and lineage commitment in the thymus requires multiple signals. Herein, CD4+ T cell generation required engagement of CD83, a surface molecule expressed by thymic epithelial and dendritic cells. CD83-deficient (CD83-/-) mice had a specific block in CD4+ single-positive thymocyte development with...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00673-6
更新日期：2002-03-22 00:00:00
abstract：:Receptor-mediated endocytosis of specific ligands is mediated through clustering of receptor-ligand complexes in coated pits on the cell surface. Following this clustering event, the ligand is internalized into a noncoated intracellular vesicle, the receptosome, which selectively avoids fusion with lysosomes and moves...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90115-4
更新日期：1980-08-01 00:00:00
abstract：:In vitro enzymatic alteration of plasmid phenotype and in vitro construction of recombinant plasmids containing genetic information derived from the plasmid pSC101 have been used to investigate the mechanism of function of tetracycline resistance determined by the plasmid pSC101. The resistance has been shown to be in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90139-3
更新日期：1978-01-01 00:00:00
abstract：:LIM domains are zinc-binding protein sequences that are found in a growing number of proteins, including certain transcriptional regulators, proto-oncogene products, and adhesion plaque constituents. Here we define the biological activity of the LIM domain through studies of an adhesion plaque protein called zyxin tha...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90191-0
更新日期：1994-10-21 00:00:00
abstract：:Members of the E2F transcription factor family (E2F-1-E2F-5) are believed to be critical positive regulators of cell cycle progression in eukaryotes although the in vivo functions of the individual E2Fs have not been elucidated. Mice were generated that lack E2F-1 and, surprisingly, these mice develop and reproduce no...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81255-6
更新日期：1996-05-17 00:00:00
abstract：:The angiotensin II (AngII) type 1 receptor (AT1R) is a critical regulator of cardiovascular and renal function and is an important model for studies of G-protein-coupled receptor (GPCR) signaling. By stabilizing the receptor with a single-domain antibody fragment ("nanobody") discovered using a synthetic yeast-display...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.12.006
更新日期：2019-01-24 00:00:00
abstract：:Pseudomonas exotoxin (PE) was used as a probe to study the mechanism by which protein ligands are internalized by mammalian cells. Both biochemical and electron microscopic methods were used to look at the internalization of PE by mouse LM cell fibroblasts. Our data suggest that PE enters cells by receptor-mediated en...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90450-x
更新日期：1980-10-01 00:00:00
abstract：:Misfolded proteins accumulating in several neurodegenerative diseases (including Alzheimer, Parkinson, and Huntington diseases) can cause aggregation of their native counterparts through a mechanism similar to the infectious prion protein's induction of a pathogenic conformation onto its cellular isoform. Evidence for...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.10.011
更新日期：2011-10-28 00:00:00
abstract：:We have analyzed two mutations of the white-eye gene, which arose in flies subject to I-R hybrid dysgenesis. These mutations are associated with insertions of apparently identical 5.4 kb sequences, which we have cloned. We believe that these insertions are copies of the I factor controlling I-R hybrid dysgenesis. The ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90536-1
更新日期：1984-08-01 00:00:00
abstract：:The trk tyrosine kinase proto-oncogene product gp140prototrk binds nerve growth factor (NGF) and is rapidly and selectively activated by this neurotrophic factor. To determine whether gp140prototrk is involved in transducing a functional NGF signal, PC12 cell mutants (PC12nnr) deficient in high affinity NGF binding an...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90441-z
更新日期：1991-09-06 00:00:00
abstract：:Poorly immunogenic tumor cells evade host immunity and grow even in the presence of an intact immune system, but the complex mechanisms regulating tumor immunogenicity have not been elucidated. Here, we discovered an unexpected role of the Hippo pathway in suppressing anti-tumor immunity. We demonstrate that, in three...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.11.005
更新日期：2016-12-01 00:00:00
abstract：:Balancing the capacity for protein maturation with changes in protein flux through the endoplasmic reticulum (ER) is crucial for maintaining ER homeostasis. In this issue, Merksamer et al. (2008) exploit a redox-sensitive fluorescent protein to monitor the environment inside the ER of living yeast, illuminating how th...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.11.004
更新日期：2008-11-28 00:00:00
abstract：:Sec7-related guanine nucleotide exchange factors (GEFs) initiate vesicle budding from the Golgi membrane surface by converting the GTPase ARF to a GTP-bound, membrane-associated form. Here we report the crystal structure of the catalytic Sec7 homology domain of Arno, a human GEF for ARF1, determined at 2.2 angstroms r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80933-2
更新日期：1998-02-06 00:00:00
abstract：:The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amoun...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90541-x
更新日期：1983-10-01 00:00:00
abstract：:Ca(2+) binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that have been primed for release by SNARE-complex assembly. Besides synaptotagmin 1, fast Ca(2+)-triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is unclear how their ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.08.030
更新日期：2006-09-22 00:00:00
abstract：:Regulated proteolysis is essential for cell cycle progression in both prokaryotes and eukaryotes. We show here that the ClpXP protease, responsible for the degradation of multiple bacterial proteins, is dynamically localized to specific cellular positions in Caulobacter where it degrades colocalized proteins. The CtrA...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.12.033
更新日期：2006-02-10 00:00:00
abstract：:We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90245-4
更新日期：1985-12-01 00:00:00
abstract：:Polycomb group (PcG) and trithorax group (trxG) proteins are critical regulators of numerous developmental genes. To silence or activate gene expression, respectively, PcG and trxG proteins bind to specific regions of DNA and direct the posttranslational modification of histones. Recent work suggests that PcG proteins...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.02.009
更新日期：2007-02-23 00:00:00
abstract：:Despite all modern advances in medicine, an effective drug treatment of obesity has not been found yet. Discovery of leptin two decades ago created hopes for treatment of obesity. However, development of leptin resistance has been a big obstacle, mitigating a leptin-centric treatment of obesity. Here, by using in sili...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.011
更新日期：2015-05-21 00:00:00
abstract：:The P7C3 class of aminopropyl carbazole chemicals fosters the survival of neurons in a variety of rodent models of neurodegeneration or nerve cell injury. To uncover its mechanism of action, an active derivative of P7C3 was modified to contain both a benzophenone for photocrosslinking and an alkyne for CLICK chemistry...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.07.040
更新日期：2014-09-11 00:00:00
