Abstract：
:Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles. It has been postulated to dock vesicles to their release sites, to act as the Ca2+ sensor for the release process, and to be a fusion protein during exocytosis. To clarify the function of this protein, we have undertaken a genetic analysis of the synaptotagmin gene in Drosophila. We have identified five lethal alleles of synaptotagmin, at least one of which lacks detectable protein. Surprisingly, however, many embryos homozygous for this null allele hatch and, as larvae, crawl, feed, and respond to stimuli. Electrophysiological recordings in embryonic cultures confirmed that synaptic transmission persists in the null allele. Therefore, synaptotagmin is not absolutely required for the regulated exocytosis of synaptic vesicles. The lethality of synaptotagmin in late first instar larvae is probably due to a perturbation of transmission that leaves the main apparatus for vesicle docking and fusion intact.
journal_name
Celljournal_title
Cellauthors
DiAntonio A,Parfitt KD,Schwarz TLdoi
10.1016/0092-8674(93)90356-usubject
Has Abstractpub_date
1993-07-02 00:00:00pages
1281-90issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(93)90356-Ujournal_volume
73pub_type
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