Abstract:
:The mitotic checkpoint prevents advance to anaphase prior to successful attachment of every centromere/kinetochore to mitotic spindle microtubules. Using purified components and Xenopus egg extracts, the kinetochore-associated microtubule motor CENP-E is now shown to be the activator of the essential checkpoint kinase BubR1. Since kinase activity and the checkpoint are silenced following CENP-E-dependent microtubule attachment in extracts or binding of CENP-E antibodies that do not disrupt CENP-E association with BubR1, CENP-E mediates silencing of BubR1 signaling. Checkpoint signaling requires the normal level of BubR1 containing a functional Mad3 domain implicated in Cdc20 binding, but only a small fraction need be kinase competent. This supports bifunctional roles for BubR1 in the checkpoint: an enzymatic one requiring CENP-E-dependent activation of its kinase activity at kinetochores and a stoichiometric one as a direct inhibitor of Cdc20.
journal_name
Celljournal_title
Cellauthors
Mao Y,Abrieu A,Cleveland DWdoi
10.1016/s0092-8674(03)00475-6subject
Has Abstractpub_date
2003-07-11 00:00:00pages
87-98issue
1eissn
0092-8674issn
1097-4172pii
S0092867403004756journal_volume
114pub_type
杂志文章相关文献
CELL文献大全abstract::Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81742-0
更新日期:1998-09-18 00:00:00
abstract::Initiator tRNAs from the cytoplasm of salmon testes and salmon liver have been purified. The nucleotide sequence of these initiator tRNAs has been determined and found identical to that of initiator tRNA from mammalian cytoplasm. The only difference is the extent of modification of the nuceloside located between the d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90189-0
更新日期:1975-11-01 00:00:00
abstract::The mitotic checkpoint ensures proper chromosome segregation by delaying anaphase until chromosomes are aligned on the spindle. Following prolonged spindle damage, however, cells eventually exit mitosis and undergo apoptosis. We show here that a murine homolog of the yeast mitotic checkpoint gene BUB1 localizes to the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80255-x
更新日期:1997-05-30 00:00:00
abstract::We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...
journal_title:Cell
pub_type: 杂志文章
doi:
更新日期:1993-10-08 00:00:00
abstract::HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00714-1
更新日期:2003-09-19 00:00:00
abstract::Sterol regulatory element binding proteins (SREBPs) are transcription factors attached to the endoplasmic reticulum. The NH2-segment, which activates transcription, is connected to membranes by a hairpin anchor formed by two transmembrane sequences and a short lumenal loop. Using H-Ras-SREBP-2 fusion proteins, we show...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81304-5
更新日期:1996-06-28 00:00:00
abstract::The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00177-x
更新日期:2000-11-22 00:00:00
abstract::The S. cerevisiae gene MCR1 encodes two mitochondrial isoforms of NADH-cytochrome b5 reductase. The primary translation product has an amino-terminal matrix-targeting signal, followed by a stretch of 21 uncharged amino acids. This precursor protein is inserted into the outer membrane, but only about one-third of the m...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90072-8
更新日期:1994-12-02 00:00:00
abstract::Previous work on Drosophila santomea suggested that its absence of abdominal pigmentation, compared to the other darkly pigmented species, is based on mutations in the cis-regulatory region of tan, inactivating the expression of that gene in the abdomen of D. santomea males and females. Our discovery that D. santomea ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.10.033
更新日期:2009-12-11 00:00:00
abstract::Mutants in bacterial topoisomerase (topo) IV are deficient in chromosomal partitioning. To investigate the basis of this phenotype, we examined plasmid DNA topology in conditionally lethal topo IV mutants. We found that dimeric catenated plasmids accumulated in vivo after topo IV inhibition. The catenanes were superco...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90356-h
更新日期:1992-10-16 00:00:00
abstract::Cell lines stably transfected with metal inducible, MT-fos chimeric genes were used to study the ability of the c-fos gene product, Fos, to act as a transcriptional trans-activator. In 3T3MTfos cells, induction of Fos expression led to specific trans-activation of an AP-1 responsive reporter gene. Induction of Fos exp...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90076-1
更新日期:1988-08-12 00:00:00
abstract::Structures of 70 unique G protein-coupled receptors (GPCRs) have been determined, with over 370 structures in total bound to different ligands and the receptors in various conformational states. Structure-based drug design has been applied to an increasing number of GPCR targets over the past decade and now a few of t...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2020.03.003
更新日期:2020-04-02 00:00:00
abstract::Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes across 32 normal human tissues. Tissue-specific or tissue...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.08.036
更新日期:2020-10-01 00:00:00
abstract::The Hippo-signaling pathway is an important regulator of cellular proliferation and organ size. However, little is known about the role of this cascade in the control of cell fate. Employing a combination of lineage tracing, clonal analysis, and organoid culture approaches, we demonstrate that Hippo pathway activity i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.060
更新日期:2014-06-05 00:00:00
abstract::Clinical trials are key to translating scientific advances into progress in cancer research and care. Confronted by developments in basic science, the landscape of clinical cancer research is rapidly evolving. Here, we review recent changes in clinical trials' design and conduct, and we forecast future directions towa...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.027
更新日期:2017-02-09 00:00:00
abstract::Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80592-9
更新日期:1999-03-19 00:00:00
abstract::Many biological motor molecules move within cells using stepsizes predictable from their structures. Myosin VI, however, has much larger and more broadly distributed stepsizes than those predicted from its short lever arms. We explain the discrepancy by monitoring Qdots and gold nanoparticles attached to the myosin-VI...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.08.033
更新日期:2010-09-17 00:00:00
abstract::Spotted fever group (SFG) rickettsiae are human pathogens that infect cells in the vasculature. They disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. Other bacterial pathogens rely on actin-based motility to provide a physical for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.09.023
更新日期:2016-10-20 00:00:00
abstract::The RNA helicase eIF4A plays a key role in unwinding of mRNA and scanning during translation initiation. Free eIF4A is a poor helicase and requires the accessory proteins eIF4G and eIF4H. However, the structure of the helicase complex and the mechanisms of stimulation of eIF4A activity have remained elusive. Here we r...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.01.014
更新日期:2009-02-06 00:00:00
abstract::The growth of lymphatic vessels (lymphangiogenesis) is actively involved in a number of pathological processes including tissue inflammation and tumor dissemination but is insufficient in patients suffering from lymphedema, a debilitating condition characterized by chronic tissue edema and impaired immunity. The recen...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2010.01.045
更新日期:2010-02-19 00:00:00
abstract::We have analyzed the structure of the Shrunken (Sh) locus in a strain containing an unstable recessive mutation, sh-m5933, caused by the transposable controlling element Dissociation (Ds). We have also analyzed nine spontaneous Sh revertant alleles. The sh-m5933 allele contains a 30 kb insertion at the Sh locus, as we...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90372-0
更新日期:1983-09-01 00:00:00
abstract::The fasciclin I, II, and III glycoproteins are expressed on different subsets of axon bundles (fascicles) in insect embryos and are thus candidates for surface recognition molecules involved in growth cone guidance. Here we present the sequence of grasshopper fasciclin I and the identification and sequence of the Dros...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90574-0
更新日期:1988-05-20 00:00:00
abstract::Given the heterogeneity of senescent cells, our knowledge of both the drivers and consequences of cellular senescence in tissues and organs remains limited, as is our understanding of how this process could be harnessed for human health. Here we identified five broad areas that would help propel the field forward. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.10.032
更新日期:2020-11-25 00:00:00
abstract::In order to identify nucleotide sequences required for efficient and accurate polyadenylation of mRNA precursors, we have constructed a series of mutations in the X. laevis beta 1-globin gene and analyzed transcripts produced upon microinjection into Xenopus oocytes. Small deletion and linker replacement mutations, wh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90743-9
更新日期:1986-07-18 00:00:00
abstract::The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression. It remains unclear how the APC - or many other E3s - promotes the multiple distinct reactions ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.05.027
更新日期:2007-07-13 00:00:00
abstract::Sleep is characterized by behavioral quiescence, homeostasis, increased arousal threshold, and rapid reversibility. Understanding how these properties are encoded by a neuronal circuit has been difficult, and no single molecular or neuronal pathway has been shown to be responsible for the regulation of sleep. Taking a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.11.036
更新日期:2014-01-16 00:00:00
abstract::Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential--presumably providing the housekeeping chaperone functions--while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.09.012
更新日期:2005-12-02 00:00:00
abstract::In familial searching in forensic genetics, a query DNA profile is tested against a database to determine whether it represents a relative of a database entrant. We examine the potential for using linkage disequilibrium to identify pairs of profiles as belonging to relatives when the query and database rely on nonover...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.09.008
更新日期:2018-10-18 00:00:00
abstract::The rps12 gene codes for chloroplast ribosomal protein S12. In the tobacco chloroplast genome (156 kbp circular DNA), exons II and III of this gene are separated by an intron of 536 bases and are present in two copies in the inverted repeat region, while exon I is located in the large single copy region at a distance ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90361-8
更新日期:1987-01-16 00:00:00
abstract::Metastasis is the leading cause of cancer-associated death but has been difficult to study because it involves a series of rare, stochastic events. To capture these events, we developed a sensitive method to tag and track pancreatic epithelial cells in a mouse model of pancreatic cancer. Tagged cells invaded and enter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.025
更新日期:2012-01-20 00:00:00