当前位置： SCI文献检索 > CELL期刊下所有文献 > Sequential E2s drive polyubiquitin chain assembly on APC targets.

Sequential E2s drive polyubiquitin chain assembly on APC targets.

Abstract：

:The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression. It remains unclear how the APC - or many other E3s - promotes the multiple distinct reactions necessary for chain assembly. We addressed this problem by analyzing APC interactions with different E2s. We screened all budding yeast E2s as APC coenzymes in vitro and found that two, Ubc4 and Ubc1, are the key E2 partners for the APC. These proteins display strikingly different but complementary enzymatic behaviors: Ubc4 supports the rapid monoubiquitination of multiple lysines on APC targets, while Ubc1 catalyzes K48-linked polyubiquitin chain assembly on preattached ubiquitins. Mitotic APC function is lost in yeast strains lacking both Ubc1 and Ubc4. E2-25K, a human homolog of Ubc1, also promotes APC-dependent chain extension on preattached ubiquitins. We propose that sequential E2 proteins catalyze K48-linked polyubiquitination and thus proteasomal destruction of APC targets.

journal_name

Cell

journal_title

Cell

authors

Rodrigo-Brenni MC,Morgan DO

doi

10.1016/j.cell.2007.05.027

subject

Has Abstract

pub_date

2007-07-13 00:00:00

pages

127-39

issue

1

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(07)00665-4

journal_volume

130

pub_type

杂志文章

相关文献

CELL文献大全
  • Communication by touch: role of cellular extensions in complex animals.

    abstract：:Neurons make networks of intercellular connections. Many other cells also send out long cellular extensions and "touch" other cells far away. The extensions are intriguing, but what do they do? Possible rationales for this cell behavior include: (1) forming precise binary connections with the possibility of forming mo...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/s0092-8674(03)00156-9

    authors： Rørth P

    更新日期：2003-03-07 00:00:00

  • Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3.

    abstract：:A cDNA clone encoding a novel hematopoietic growth factor activity produced by a gibbon T cell line has been identified using a mammalian cell expression cloning system. The sequence of this cDNA proved to have significant homology to the sequence encoding murine interleukin 3 (IL-3). The human gene, which was readily...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(86)90360-0

    authors： Yang YC,Ciarletta AB,Temple PA,Chung MP,Kovacic S,Witek-Giannotti JS,Leary AC,Kriz R,Donahue RE,Wong GG

    更新日期：1986-10-10 00:00:00

  • Wnt Down, Tumors Wind Up?

    abstract：:In mouse intestinal tumors induced by the inhibition of APC, the restoration of APC function causes complete tumor regression with normal differentiation and return of stem cell function irrespective of whether tumors also carried mutations in Kras and p53. These findings by Dow et al. validate the Wnt pathway as an e...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2015.06.008

    authors： Krimpenfort P,Berns A

    更新日期：2015-06-18 00:00:00

  • The existence of an insoluble Z disc scaffold in chicken skeletal muscle.

    abstract：:Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(78)90051-x

    authors： Granger BL,Lazarides E

    更新日期：1978-12-01 00:00:00

  • A 450 kb transgene displays properties of the mammalian X-inactivation center.

    abstract：:X inactivation results in inactivation of one X chromosome to compensate for gene dosage differences between mammalian females and males. It requires the X-inactivation center (Xic) and Xist in cis. We report that introducing 450 kb of murine Xic/Xist sequences onto autosomes activates female dosage compensation in ma...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)80079-3

    authors： Lee JT,Strauss WM,Dausman JA,Jaenisch R

    更新日期：1996-07-12 00:00:00

  • Hippo pathway activity influences liver cell fate.

    abstract：:The Hippo-signaling pathway is an important regulator of cellular proliferation and organ size. However, little is known about the role of this cascade in the control of cell fate. Employing a combination of lineage tracing, clonal analysis, and organoid culture approaches, we demonstrate that Hippo pathway activity i...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.03.060

    authors： Yimlamai D,Christodoulou C,Galli GG,Yanger K,Pepe-Mooney B,Gurung B,Shrestha K,Cahan P,Stanger BZ,Camargo FD

    更新日期：2014-06-05 00:00:00

  • A bivalent tarantula toxin activates the capsaicin receptor, TRPV1, by targeting the outer pore domain.

    abstract：:Toxins have evolved to target regions of membrane ion channels that underlie ligand binding, gating, or ion permeation, and have thus served as invaluable tools for probing channel structure and function. Here, we describe a peptide toxin from the Earth Tiger tarantula that selectively and irreversibly activates the c...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2010.03.052

    authors： Bohlen CJ,Priel A,Zhou S,King D,Siemens J,Julius D

    更新日期：2010-05-28 00:00:00

  • Role of small t antigen in the acute transforming activity of SV40.

    abstract：:A plasmid, pHR402, containing SV40 sequences that include a truncated early region bearing an intact t-coding sequence and a functionally intact late region, was introduced into thymidine kinase deficient (tk-) mouse L cells by cotransformation with a cloned tk gene. tk+ cotransformants synthesized SV40 t but not T an...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90244-6

    authors： Rubin H,Figge J,Bladon MT,Chen LB,Ellman M,Bikel I,Farrell M,Livingston DM

    更新日期：1982-09-01 00:00:00

  • Trp'ing tuberculosis.

    abstract：:IFN-γ-activated macrophages attempt to limit pathogen growth through tryptophan starvation. Zhang et al. demonstrate that Mycobacterium tuberculosis responds to this threat by synthesizing its own tryptophan. Genetic and chemical inhibition of this pathway reduces mycobacterial survival, revealing a new immune-depende...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2013.11.015

    authors： Russell DG

    更新日期：2013-12-05 00:00:00

  • A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs.

    abstract：:The alpha-amylase mRNAs which accumulate in two different tissues of the mouse, the salivary gland and the liver, are identical except for their 5' non-translated sequences: the 5' terminal 158 nucleotides of the major liver alpha-amylase mRNA are unrelated to the 5' terminal 47 nucleotides found in its salivary gland...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90140-9

    authors： Young RA,Hagenbüchle O,Schibler U

    更新日期：1981-02-01 00:00:00

  • Specified neural progenitors sort to form sharp domains after noisy Shh signaling.

    abstract：:Sharply delineated domains of cell types arise in developing tissues under instruction of inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. The translation of a morphogen gradient into discrete spatial domains relies on precise signal responses at stable cell positio...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2013.03.023

    authors： Xiong F,Tentner AR,Huang P,Gelas A,Mosaliganti KR,Souhait L,Rannou N,Swinburne IA,Obholzer ND,Cowgill PD,Schier AF,Megason SG

    更新日期：2013-04-25 00:00:00

  • Aralar Sequesters GABA into Hyperactive Mitochondria, Causing Social Behavior Deficits.

    abstract：:Social impairment is frequently associated with mitochondrial dysfunction and altered neurotransmission. Although mitochondrial function is crucial for brain homeostasis, it remains unknown whether mitochondrial disruption contributes to social behavioral deficits. Here, we show that Drosophila mutants in the homolog ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.02.044

    authors： Kanellopoulos AK,Mariano V,Spinazzi M,Woo YJ,McLean C,Pech U,Li KW,Armstrong JD,Giangrande A,Callaerts P,Smit AB,Abrahams BS,Fiala A,Achsel T,Bagni C

    更新日期：2020-03-19 00:00:00

  • Proteogenomic Landscape of Breast Cancer Tumorigenesis and Targeted Therapy.

    abstract：:The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued to preserve post-translational modifications, including protein phosphorylation a...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.10.036

    authors： Krug K,Jaehnig EJ,Satpathy S,Blumenberg L,Karpova A,Anurag M,Miles G,Mertins P,Geffen Y,Tang LC,Heiman DI,Cao S,Maruvka YE,Lei JT,Huang C,Kothadia RB,Colaprico A,Birger C,Wang J,Dou Y,Wen B,Shi Z,Liao Y,Wizn

    更新日期：2020-11-25 00:00:00

  • Premature of chromosome condensation in a ts DNA- mutant of BHK cells.

    abstract：:A temperature-sensitive mutant of BHK, designated ts BN-2, shows a rapid drop in 3H-thymidine incorporation along with accumulation of the cells in the G1 phase of the cycle when asynchronous cultures are shifted from 33.5 degrees C to the nonpermissive temperature of 39.5 degrees C. Synchronized cultures of ts BN-2 c...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(78)90017-x

    authors： Nishimoto T,Eilen E,Basilico C

    更新日期：1978-10-01 00:00:00

  • Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging.

    abstract：:During aging, stromal functions are thought to be impaired, but little is known whether this stems from changes of fibroblasts. Using population- and single-cell transcriptomics, as well as long-term lineage tracing, we studied whether murine dermal fibroblasts are altered during physiological aging under different di...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2018.10.012

    authors： Salzer MC,Lafzi A,Berenguer-Llergo A,Youssif C,Castellanos A,Solanas G,Peixoto FO,Stephan-Otto Attolini C,Prats N,Aguilera M,Martín-Caballero J,Heyn H,Benitah SA

    更新日期：2018-11-29 00:00:00

  • Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters.

    abstract：:Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90185-9

    authors： Miura A,Krueger JH,Itoh S,de Boer HA,Nomura M

    更新日期：1981-09-01 00:00:00

  • Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.

    abstract：:We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(84)90225-3

    authors： Malissen B,Price MP,Goverman JM,McMillan M,White J,Kappler J,Marrack P,Pierres A,Pierres M,Hood L

    更新日期：1984-02-01 00:00:00

  • Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.

    abstract：:In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(92)90443-g

    authors： Berkenstam A,Vivanco Ruiz MM,Barettino D,Horikoshi M,Stunnenberg HG

    更新日期：1992-05-01 00:00:00

  • Behind the movement.

    abstract：:This year, the Albert Lasker Basic Medical Research Award will be shared by Michael Sheetz, James Spudich, and Ronald Vale for discoveries concerning the biophysical actions of cytoskeletal motor-protein machines that move cargo within cells, contract muscles, and enable cell motility. ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2012.08.031

    authors： Ringe D,Petsko GA

    更新日期：2012-09-14 00:00:00

  • A myristoyl/phosphotyrosine switch regulates c-Abl.

    abstract：:The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(03)00191-0

    authors： Hantschel O,Nagar B,Guettler S,Kretzschmar J,Dorey K,Kuriyan J,Superti-Furga G

    更新日期：2003-03-21 00:00:00

  • Histone methylation-dependent mechanisms impose ligand dependency for gene activation by nuclear receptors.

    abstract：:Nuclear receptors undergo ligand-dependent conformational changes that are required for corepressor-coactivator exchange, but whether there is an actual requirement for specific epigenetic landmarks to impose ligand dependency for gene activation remains unknown. Here we report an unexpected and general strategy that ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2006.12.038

    authors： Garcia-Bassets I,Kwon YS,Telese F,Prefontaine GG,Hutt KR,Cheng CS,Ju BG,Ohgi KA,Wang J,Escoubet-Lozach L,Rose DW,Glass CK,Fu XD,Rosenfeld MG

    更新日期：2007-02-09 00:00:00

  • Function of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing requires an idiosyncratic domain not found in other synthetases.

    abstract：:Neurospora mitochondrial tyrosyl-tRNA synthetase (mt TyrRS), which is encoded by nuclear gene cyt-18, functions in splicing group I introns. Analysis of intragenic partial revertants of the cyt-18-2 mutant and in vitro mutants of the cyt-18 protein expressed in E. coli showed that splicing activity of the cyt-18 prote...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90119-y

    authors： Cherniack AD,Garriga G,Kittle JD Jr,Akins RA,Lambowitz AM

    更新日期：1990-08-24 00:00:00

  • Use of a cDNA library for studies on evolution and developmental expression of the chorion multigene families.

    abstract：:A cDNA library has been constructed from an RNA preparation highly enriched in silkmoth chorion mRNAs. Many distinct clones have been identified from this library using a stepwise procedure: scoring for infrequent hexanucleotide restriction enzyme recognition sequences; detailed characterization with restriction enzym...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(79)90241-1

    authors： Sim GK,Kafatos FC,Jones CW,Koehler MD,Efstratiadis A,Maniatis T

    更新日期：1979-12-01 00:00:00

  • A Viral Immunoevasin Controls Innate Immunity by Targeting the Prototypical Natural Killer Cell Receptor Family.

    abstract：:Natural killer (NK) cells play a key role in innate immunity by detecting alterations in self and non-self ligands via paired NK cell receptors (NKRs). Despite identification of numerous NKR-ligand interactions, physiological ligands for the prototypical NK1.1 orphan receptor remain elusive. Here, we identify a viral ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.03.002

    authors： Aguilar OA,Berry R,Rahim MMA,Reichel JJ,Popović B,Tanaka M,Fu Z,Balaji GR,Lau TNH,Tu MM,Kirkham CL,Mahmoud AB,Mesci A,Krmpotić A,Allan DSJ,Makrigiannis AP,Jonjić S,Rossjohn J,Carlyle JR

    更新日期：2017-03-23 00:00:00

  • Production of a DPP activity gradient in the early Drosophila embryo through the opposing actions of the SOG and TLD proteins.

    abstract：:During early Drosophila embryogenesis, several zygotic gene products act to establish a posttranscriptional activity gradient of the morphogen DPP. Among these molecules, Tolloid, a putative metalloprotease related to BMP-1, enhances DPP function, while SOG, an ortholog of the Xenopus organizer Chordin, inhibits DPP f...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)80425-0

    authors： Marqués G,Musacchio M,Shimell MJ,Wünnenberg-Stapleton K,Cho KW,O'Connor MB

    更新日期：1997-10-31 00:00:00

  • Activation of STAT6 by STING is critical for antiviral innate immunity.

    abstract：:STAT6 plays a prominent role in adaptive immunity by transducing signals from extracellular cytokines. We now show that STAT6 is required for innate immune signaling in response to virus infection. Viruses or cytoplasmic nucleic acids trigger STING (also named MITA/ERIS) to recruit STAT6 to the endoplasmic reticulum, ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2011.09.022

    authors： Chen H,Sun H,You F,Sun W,Zhou X,Chen L,Yang J,Wang Y,Tang H,Guan Y,Xia W,Gu J,Ishikawa H,Gutman D,Barber G,Qin Z,Jiang Z

    更新日期：2011-10-14 00:00:00

  • Structurally Resolved SARS-CoV-2 Antibody Shows High Efficacy in Severely Infected Hamsters and Provides a Potent Cocktail Pairing Strategy.

    abstract：:Understanding how potent neutralizing antibodies (NAbs) inhibit SARS-CoV-2 is critical for effective therapeutic development. We previously described BD-368-2, a SARS-CoV-2 NAb with high potency; however, its neutralization mechanism is largely unknown. Here, we report the 3.5-Å cryo-EM structure of BD-368-2/trimeric-...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.09.035

    authors： Du S,Cao Y,Zhu Q,Yu P,Qi F,Wang G,Du X,Bao L,Deng W,Zhu H,Liu J,Nie J,Zheng Y,Liang H,Liu R,Gong S,Xu H,Yisimayi A,Lv Q,Wang B,He R,Han Y,Zhao W,Bai Y,Qu Y,Gao X,Ji C,Wang Q,Gao N,Huang W,Wang

    更新日期：2020-11-12 00:00:00

  • N(6)-methyladenosine Modulates Messenger RNA Translation Efficiency.

    abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification in mammalian mRNA. This modification is reversible and non-stoichiometric and adds another layer to the dynamic control of mRNA metabolism. The stability of m(6)A-modified mRNA is regulated by an m(6)A reader protein, human YTHDF2, which recognize...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2015.05.014

    authors： Wang X,Zhao BS,Roundtree IA,Lu Z,Han D,Ma H,Weng X,Chen K,Shi H,He C

    更新日期：2015-06-04 00:00:00

  • A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

    abstract：:The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2015.09.053

    authors： Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

    更新日期：2015-10-22 00:00:00

  • A mechanism for gene-environment interaction in the etiology of congenital scoliosis.

    abstract：:Congenital scoliosis, a lateral curvature of the spine caused by vertebral defects, occurs in approximately 1 in 1,000 live births. Here we demonstrate that haploinsufficiency of Notch signaling pathway genes in humans can cause this congenital abnormality. We also show that in a mouse model, the combination of this g...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2012.02.054

    authors： Sparrow DB,Chapman G,Smith AJ,Mattar MZ,Major JA,O'Reilly VC,Saga Y,Zackai EH,Dormans JP,Alman BA,McGregor L,Kageyama R,Kusumi K,Dunwoodie SL

    更新日期：2012-04-13 00:00:00