Abstract：
:Syntaxin 1A plays a central role in neurotransmitter release through multiple protein-protein interactions. We have used NMR spectroscopy to identify an autonomously folded N-terminal domain in syntaxin 1A and to elucidate its three-dimensional structure. This 120-residue N-terminal domain is conserved in plasma membrane syntaxins but not in other syntaxins, indicating a specific role in exocytosis. The domain contains three long alpha helices that form an up-and-down bundle with a left-handed twist. A striking residue conservation is observed throughout a long groove that is likely to provide a specific surface for protein-protein interactions. A highly acidic region binds to the C2A domain of synaptotagmin I in a Ca2+-dependent interaction that may serve as an electrostatic switch in neurotransmitter release.
journal_name
Celljournal_title
Cellauthors
Fernandez I,Ubach J,Dulubova I,Zhang X,Südhof TC,Rizo Jdoi
10.1016/s0092-8674(00)81742-0subject
Has Abstractpub_date
1998-09-18 00:00:00pages
841-9issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81742-0journal_volume
94pub_type
杂志文章相关文献
CELL文献大全abstract：:The intestinal epithelium is a highly structured tissue composed of repeating crypt-villus units. Enterocytes perform the diverse tasks of absorbing a wide range of nutrients while protecting the body from the harsh bacterium-rich environment. It is unknown whether these tasks are spatially zonated along the villus ax...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.08.063
更新日期：2018-11-01 00:00:00
abstract：:The preprotein translocase of the outer membrane of mitochondria (TOM complex) facilitates the recognition, insertion, and translocation of nuclear-encoded mitochondrial preproteins. We have purified the TOM complex from Neurospora crassa and analyzed its composition and functional properties. The TOM complex contains...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81206-4
更新日期：1998-06-12 00:00:00
abstract：:Loss of cell polarity proteins such as Scribble induces neoplasia in Drosophila by promoting uncontrolled proliferation. In mammals, the role that polarity proteins play during tumorigenesis is not well understood. Here, we demonstrate that depletion of Scribble in mammary epithelia disrupts cell polarity, blocks thre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.045
更新日期：2008-11-28 00:00:00
abstract：:We have analyzed the structure of the Shrunken (Sh) locus in a strain containing an unstable recessive mutation, sh-m5933, caused by the transposable controlling element Dissociation (Ds). We have also analyzed nine spontaneous Sh revertant alleles. The sh-m5933 allele contains a 30 kb insertion at the Sh locus, as we...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90372-0
更新日期：1983-09-01 00:00:00
abstract：:Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of unde...
journal_title：Cell
pub_type： 历史文章,杂志文章
doi：10.1016/j.cell.2012.01.002
更新日期：2012-01-20 00:00:00
abstract：:In Xenopus development, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. CPEB and maskin, two factors that control polyadenylation-induced translation are present on the mitotic apparatus of animal pole blastomeres in embryos. Cyclin B1 protein and mRNA, whose translation is regula...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00135-5
更新日期：2000-10-27 00:00:00
abstract：:Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directe...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90012-2
更新日期：1987-10-09 00:00:00
abstract：:We have adopted Drosophila tissue culture cells as a host system for studying the structure and function of mammalian transcription factors. These cells provide an Sp1-deficient background and have been used in a complementation assay to identify functional domains of human transcription factor Sp1. The SV40 early pro...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90144-4
更新日期：1988-12-02 00:00:00
abstract：:Hsp33, a member of a newly discovered heat shock protein family, was found to be a very potent molecular chaperone. Hsp33 is distinguished from all other known molecular chaperones by its mode of functional regulation. Its activity is redox regulated. Hsp33 is a cytoplasmically localized protein with highly reactive c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80547-4
更新日期：1999-02-05 00:00:00
abstract：:A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90230-1
更新日期：1983-11-01 00:00:00
abstract：:The short chain aliphatic acid salts, butyrate and propionate, are effective inhibitors of histone deacetylation in chick oviduct at 2--5 mM; they also prevent the hormonal induction of the ovalbumin and transferrin genes. The less potent deacetylase inhibitor isobutyrate is correspondingly less effective in blocking ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90357-8
更新日期：1980-11-01 00:00:00
abstract：:Monkey cell lines that constitutively synthesize 38.6 kd lac repressor protein and bear stably integrated chloramphenicol acetyl transferase (CAT) genes linked to a lac operator-containing SV40 early promoter-enhancer were generated. When grown in medium containing isopropyl beta-D-thiogalactoside (IPTG), these cells ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90409-6
更新日期：1988-03-11 00:00:00
abstract：:Genetic alterations in signaling pathways that control cell-cycle progression, apoptosis, and cell growth are common hallmarks of cancer, but the extent, mechanisms, and co-occurrence of alterations in these pathways differ between individual tumors and tumor types. Using mutations, copy-number changes, mRNA expressio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.03.035
更新日期：2018-04-05 00:00:00
abstract：:ROAM mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT alpha diploid strains which cannot conjugate than in haplolid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproductio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90353-0
更新日期：1980-11-01 00:00:00
abstract：:Defects in the gene encoding the endothelin-B receptor produce aganglionic megacolon and pigmentary disorders in mice and humans. We report that a targeted disruption of the mouse endothelin-3 ligand (EDN3) gene produces a similar recessive phenotype of megacolon and coat color spotting. A natural recessive mutation t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90018-3
更新日期：1994-12-30 00:00:00
abstract：:The association of histone modification changes with autism spectrum disorder (ASD) has not been systematically examined. We conducted a histone acetylome-wide association study (HAWAS) by performing H3K27ac chromatin immunoprecipitation sequencing (ChIP-seq) on 257 postmortem samples from ASD and matched control brai...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.10.031
更新日期：2016-11-17 00:00:00
abstract：:Phosphoinositide 3-kinases (PI3-Ks) are an important emerging class of drug targets, but the unique roles of PI3-K isoforms remain poorly defined. We describe here an approach to pharmacologically interrogate the PI3-K family. A chemically diverse panel of PI3-K inhibitors was synthesized, and their target selectivity...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.03.035
更新日期：2006-05-19 00:00:00
abstract：:We have shown that lariat formation during in vitro splicing of several RNA precursors, from Drosophila to man, occurs at a unique and identifiable but weakly conserved site, 18 to 37 nucleotides proximal to the 3' splice site. Lariat formation within an artificial intron lacking a normal branch-point sequence occurs ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90064-9
更新日期：1985-05-01 00:00:00
abstract：:Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) σ(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-Å-resolut...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.10.034
更新日期：2012-11-21 00:00:00
abstract：:Lipid biology continues to emerge as an area of significant therapeutic interest, particularly as the result of an enhanced understanding of the wealth of signaling molecules with diverse physiological properties. This growth in knowledge is epitomized by lysophosphatidic acid (LPA), which functions through interactio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.06.002
更新日期：2015-06-18 00:00:00
abstract：:Distinct classes of neurons are generated at defined positions in the ventral neural tube in response to a gradient of Sonic Hedgehog (Shh) activity. A set of homeodomain transcription factors expressed by neural progenitors act as intermediaries in Shh-dependent neural patterning. These homeodomain factors fall into ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80853-3
更新日期：2000-05-12 00:00:00
abstract：:Generation of potent antibodies by a mutation-selection process called affinity maturation is a key component of effective immune responses. Antibodies that protect against highly mutable pathogens must neutralize diverse strains. Developing effective immunization strategies to drive their evolution requires understan...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.01.027
更新日期：2015-02-12 00:00:00
abstract：:Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.01.015
更新日期：2010-01-22 00:00:00
abstract：:A model of hematopoietic development wherein multipotentiality is conserved until segregation of myeloid and lymphoid potential has recently been challenged, proposing that megakaryocyte/erythrocyte (MegE) potential is lost in Flk2/Flt3-expressing early progenitors. Here, we used sensitive in vivo approaches to quanti...
journal_title：Cell
pub_type： 信件
doi：10.1016/j.cell.2006.06.037
更新日期：2006-07-28 00:00:00
abstract：:It remains largely unknown how large-scale tissue movements during development emerge from the interplay of different tensile forces associated with actomyosin networks. Solon et al. (2009) now report that a ratchet-like mechanism drives the movement of epithelial sheets during dorsal closure in embryos of the fruit f...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.06.009
更新日期：2009-06-26 00:00:00
abstract：:We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81164-2
更新日期：1998-05-01 00:00:00
abstract：:In this issue, Stetson et al. (2008) report a mechanism by which host cells avert an autoimmune response to self-nucleic acids. They show that the nuclease Trex1 prevents the accumulation of DNA derived from endogenous retroelements that, if left unchecked, trigger elevated production of type I interferons leading to ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.08.010
更新日期：2008-08-22 00:00:00
abstract：:The Bcl-2 family member Bax translocates from the cytosol to mitochondria, where it oligomerizes and permeabilizes the mitochondrial outer membrane to promote apoptosis. Bax activity is counteracted by prosurvival Bcl-2 proteins, but how they inhibit Bax remains controversial because they neither colocalize nor form s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.02.034
更新日期：2011-04-01 00:00:00
abstract：:The ephrin/Eph system plays a central role in neuronal circuit formation; however, its downstream effectors are poorly understood. Here we show that alpha-chimerin Rac GTPase-activating protein mediates ephrinB3/EphA4 forward signaling. We discovered a spontaneous mouse mutation, miffy (mfy), which results in a rabbit...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.07.022
更新日期：2007-08-24 00:00:00
abstract：:Hox homeodomain proteins are developmental regulators that determine body plan in a variety of organisms. A majority of the vertebrate Hox proteins bind DNA as heterodimers with the Pbx1 homeodomain protein. We report here the 2.35 A structure of a ternary complex containing a human HoxB1-Pbx1 heterodimer bound to DNA...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80662-5
更新日期：1999-02-19 00:00:00
