Abstract：
:The intestinal epithelium is a highly structured tissue composed of repeating crypt-villus units. Enterocytes perform the diverse tasks of absorbing a wide range of nutrients while protecting the body from the harsh bacterium-rich environment. It is unknown whether these tasks are spatially zonated along the villus axis. Here, we extracted a large panel of landmark genes characterized by transcriptomics of laser capture microdissected villus segments and utilized it for single-cell spatial reconstruction, uncovering broad zonation of enterocyte function along the villus. We found that enterocytes at villus bottoms express an anti-bacterial gene program in a microbiome-dependent manner. They next shift to sequential expression of carbohydrates, peptides, and fat absorption machineries in distinct villus compartments. Finally, they induce a Cd73 immune-modulatory program at the villus tips. Our approach can be used to uncover zonation patterns in other organs when prior knowledge of landmark genes is lacking.
journal_name
Celljournal_title
Cellauthors
Moor AE,Harnik Y,Ben-Moshe S,Massasa EE,Rozenberg M,Eilam R,Bahar Halpern K,Itzkovitz Sdoi
10.1016/j.cell.2018.08.063subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1156-1167.e15issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(18)31164-4journal_volume
175pub_type
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