Abstract:
:ROAM mutations cause overproduction in S. cerevisiae. Overproduction of ROAM mutant gene products is less in MATa/MAT alpha diploid strains which cannot conjugate than in haplolid strains which can. Overproduction occurs in diploid strains capable of mating whether or not they are capable of sporulating. Overproduction decreases when haploid ROAM mutants also contain the ste7 mutation which prevents conjugation; other ste mutations do not affect the expression of ROAM mutations. Cloning of the ROAM mutant gene CYC7-H2 shows that a 5.5 kb sequence homologous to a transposable and reiterated Ty1 element is inserted in the 5' noncoding region of the CYC7 structural locus. The similar genetic properties of other ROAM mutations suggest that they each contain an inserted Ty element. These results also suggest that ROAM mutations respond to signals normally directed toward genes controlling conjugation functions, and that sequences present in Ty elements may be adjacent to structural loci and are the normal receptors for these signals.
journal_name
Celljournal_title
Cellauthors
Errede B,Cardillo TS,Sherman F,Dubois E,Deschamps J,Wiame JMdoi
10.1016/0092-8674(80)90353-0subject
Has Abstractpub_date
1980-11-01 00:00:00pages
427-36issue
2 Pt 2eissn
0092-8674issn
1097-4172pii
0092-8674(80)90353-0journal_volume
22pub_type
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