Abstract:
:Dysfunction and loss of insulin-producing pancreatic beta cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38delta display improved glucose tolerance due to enhanced insulin secretion from pancreatic beta cells. Deletion of p38delta results in pronounced activation of protein kinase D (PKD), the latter of which we have identified as a pivotal regulator of stimulated insulin exocytosis. p38delta catalyzes an inhibitory phosphorylation of PKD1, thereby attenuating stimulated insulin secretion. In addition, p38delta null mice are protected against high-fat-feeding-induced insulin resistance and oxidative stress-mediated beta cell failure. Inhibition of PKD1 reverses enhanced insulin secretion from p38delta-deficient islets and glucose tolerance in p38delta null mice as well as their susceptibility to oxidative stress. In conclusion, the p38delta-PKD pathway integrates regulation of the insulin secretory capacity and survival of pancreatic beta cells, pointing to a pivotal role for this pathway in the development of overt diabetes mellitus.
journal_name
Celljournal_title
Cellauthors
Sumara G,Formentini I,Collins S,Sumara I,Windak R,Bodenmiller B,Ramracheya R,Caille D,Jiang H,Platt KA,Meda P,Aebersold R,Rorsman P,Ricci Rdoi
10.1016/j.cell.2008.11.018subject
Has Abstractpub_date
2009-01-23 00:00:00pages
235-48issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(08)01493-1journal_volume
136pub_type
杂志文章相关文献
CELL文献大全abstract::Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12. Four structurally different recombinant plasmid DNAs from transformed clones were charac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90202-6
更新日期:1976-04-01 00:00:00
abstract::Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90051-x
更新日期:1978-12-01 00:00:00
abstract::All recombination models postulate one or more recombination intermediates that are joint molecules containing two homologous parental molecules. A spike of branched DNA molecules not seen in DNA from mitotic cells was found in the two-dimensional gel analysis of meiotic DNA from S. cerevisiae. The mass of molecules i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90173-2
更新日期:1994-01-14 00:00:00
abstract::At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.03.023
更新日期:2012-04-13 00:00:00
abstract::During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.08.001
更新日期:2019-09-19 00:00:00
abstract::Transfection experiments with HSV 1 in which one uses herpes simplex virus (HSV) thymidine kinase (TK) as a selectable prototrophic marker yield two classes of transformed cells: stable and unstable. In this report, we test the hypothesis that the stability phenotype can be explained by virus genome integration into a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90015-6
更新日期:1978-10-01 00:00:00
abstract::Xenopus laevis oocytes were used as a model system to study the intracellular fate of proteins incorporated by endocytosis. We found that the intracellular stability and compartmentation of proteins incorporated by receptor-mediated endocytosis differed substantially from that of proteins incorporated by nonspecific e...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90153-1
更新日期:1980-11-01 00:00:00
abstract::The orderly reduction in chromosome number that occurs during meiosis depends on two aspects of chromosome behavior specific to the first meiotic division. These are the retention of cohesion between sister centromeres and their attachment to microtubules that extend to the same pole (monopolar attachment). By deletin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00217-8
更新日期:2000-12-22 00:00:00
abstract::Understanding how transcriptional enhancers control over 20,000 protein-coding genes to maintain cell-type-specific gene expression programs in all human cells is a fundamental challenge in regulatory biology. Recent studies suggest that gene regulatory elements and their target genes generally occur within insulated ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.10.024
更新日期:2016-11-17 00:00:00
abstract::Cellular ras activity has been neutralized in 3T3 cells by microinjection of a specific anti-ras monoclonal antibody. The injected antibody efficiently inhibited proliferation in cells treated with a phorbol ester and a calcium ionophore, or with prostaglandin F2 alpha. These treatments were designed to imitate the ac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(88)90531-4
更新日期:1988-01-15 00:00:00
abstract::Morphogen gradients are typically analyzed from static images of fixed embryonic tissues. Two papers in this issue of Cell now report live imaging of the Bicoid gradient in developing fruit fly embryos (Gregor et al., 2007a, 2007b). Their findings indicate that the gradient is highly reproducible from embryo to embryo...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.06.036
更新日期:2007-07-13 00:00:00
abstract::Vascular permeability is frequently associated with inflammation and is triggered by a cohort of secreted permeability factors such as vascular endothelial growth factor (VEGF). Here, we show that the physiological vascular permeability that precedes implantation is directly controlled by progesterone receptor (PR) an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.12.025
更新日期:2014-01-30 00:00:00
abstract::Synaptotagmin is one of the major integral membrane proteins of synaptic vesicles. It has been postulated to dock vesicles to their release sites, to act as the Ca2+ sensor for the release process, and to be a fusion protein during exocytosis. To clarify the function of this protein, we have undertaken a genetic analy...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90356-u
更新日期:1993-07-02 00:00:00
abstract::We have isolated the genes encoding the largest subunit of all three classes of RNA polymerase from Trypanosoma brucei. While the pol II largest subunit is encoded by a single gene in all organisms examined to date, trypanosomes contain two copies of the gene. Both genes are expressed in the procyclic and bloodstream ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90686-7
更新日期:1989-03-10 00:00:00
abstract::The dorsal raphe nucleus (DRN) is an important brain area for body-weight regulation. In this issue of Cell, Nectow et al. uncover cell-type-specific neural circuitry and pharmacology for appetite control within the DRN. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.07.017
更新日期:2017-07-27 00:00:00
abstract::We have examined the conformation of DNA cloned from the hypersensitive region 5' of the chicken adult beta-globin gene, in order to determine whether the unusual sensitivity to nucleases is related to altered secondary structure of the DNA. The region contains a large number of sites for methylation; we have studied ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90180-0
更新日期:1983-12-01 00:00:00
abstract::Some Ts in nuclear DNA of trypanosomes and Leishmania are hydroxylated and glucosylated to yield base J (β-D-glucosyl-hydroxymethyluracil). In Leishmania, about 99% of J is located in telomeric repeats. We show here that most of the remaining J is located at chromosome-internal RNA polymerase II termination sites. Thi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.07.030
更新日期:2012-08-31 00:00:00
abstract::Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90557-x
更新日期:1986-06-20 00:00:00
abstract::Here we characterize the T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kd under reducing ones. Two of the three bands that are precipitated from metabolically labeled cells are expressed ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90593-3
更新日期:1986-11-07 00:00:00
abstract::Rat liver interphase chromosomes have telomeres 20-100 kb in length. Micrococcal nuclease digestion of nuclei cleaves telomeres with a uniform 157 bp periodicity, producing soluble particles that sediment in sucrose gradients exactly like oligonucleosomes. The monomeric telomere particles comigrate with nucleosome cor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90256-p
更新日期:1993-05-21 00:00:00
abstract::Isolation of genes encoding the receptors for steroids, retinoids, vitamin D, and thyroid hormone and their structural and functional analysis revealed an evolutionarily conserved template for nuclear hormone receptors. This discovery sparked identification of numerous genes encoding related proteins, termed orphan re...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2014.03.012
更新日期:2014-03-27 00:00:00
abstract::Signaling by the epidermal growth factor receptor requires an allosteric interaction between the kinase domains of two receptors, whereby one activates the other. We show that the intracellular juxtamembrane segment of the receptor, known to potentiate kinase activity, is able to dimerize the kinase domains. The C-ter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.04.025
更新日期:2009-06-26 00:00:00
abstract::Protein-DNA binding is mediated by the recognition of the chemical signatures of the DNA bases and the 3D shape of the DNA molecule. Because DNA shape is a consequence of sequence, it is difficult to dissociate these modes of recognition. Here, we tease them apart in the context of Hox-DNA binding by mutating residues...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.02.008
更新日期:2015-04-09 00:00:00
abstract::Microbes that cause persistent infections (e.g., herpes viruses) do so by switching from fast-growing lytic states to slow-growing latent states. Waldman et al. have identified a single transcription factor that governs the switch between the lytic and latent forms of Toxoplasma gondii, a parasite that causes a persis...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2019.12.038
更新日期:2020-01-23 00:00:00
abstract::Clinical trials are key to translating scientific advances into progress in cancer research and care. Confronted by developments in basic science, the landscape of clinical cancer research is rapidly evolving. Here, we review recent changes in clinical trials' design and conduct, and we forecast future directions towa...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.027
更新日期:2017-02-09 00:00:00
abstract::The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90405-f
更新日期:1993-03-12 00:00:00
abstract::Sexual reproduction is almost universal in eukaryotic life and involves the fusion of male and female haploid gametes into a diploid cell. The sperm-restricted single-pass transmembrane protein HAP2-GCS1 has been postulated to function in membrane merger. Its presence in the major eukaryotic taxa-animals, plants, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.01.024
更新日期:2017-02-23 00:00:00
abstract::S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81084-3
更新日期:1996-04-05 00:00:00
abstract::The peripheral nervous system has remarkable regenerative capacities in that it can repair a fully cut nerve. This requires Schwann cells to migrate collectively to guide regrowing axons across a 'bridge' of new tissue, which forms to reconnect a severed nerve. Here we show that blood vessels direct the migrating cord...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.07.021
更新日期:2015-08-27 00:00:00
abstract::As more plant genome sequences become available, researchers are increasingly using comparative genomics to address some of the major questions in plant biology. Such questions include the evolution of photosynthesis and multicellularity, the developmental genetic changes responsible for alterations in body plan, and ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.04.004
更新日期:2007-04-20 00:00:00