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In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure.

Abstract：

:We have isolated the genes encoding the largest subunit of all three classes of RNA polymerase from Trypanosoma brucei. While the pol II largest subunit is encoded by a single gene in all organisms examined to date, trypanosomes contain two copies of the gene. Both genes are expressed in the procyclic and bloodstream stages of the trypanosome life cycle. The two pol II genes differ from one another in their coding sequences by 21 silent substitutions and 4 amino acid substitutions. In the core part of the large subunit, the predicted polypeptides are similar to other eukaryotic RNA polymerases. Both trypanosome pol II polypeptides, like those of other eukaryotes, also have a unique C-terminal extension. However, this domain in the trypanosome polypeptides, unlike those of other eukaryotes, is not a tandemly repeated heptapeptide sequence.

journal_name

Cell

journal_title

Cell

authors

Smith JL,Levin JR,Ingles CJ,Agabian N

doi

10.1016/0092-8674(89)90686-7

subject

Has Abstract

pub_date

1989-03-10 00:00:00

pages

815-27

issue

5

eissn

0092-8674

issn

1097-4172

pii

0092-8674(89)90686-7

journal_volume

56

pub_type

杂志文章

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