Abstract:
:The mutated gene responsible for the tubby obesity phenotype has been identified by positional cloning. A single base change within a splice donor site results in the incorrect retention of a single intron in the mature tub mRNA transcript. The consequence of this mutation is the substitution of the carboxy-terminal 44 amino acids with 24 intron-encoded amino acids. The normal transcript appears to be abundantly expressed in the hypothalamus, a region of the brain involved in body weight regulation. Variation in the relative abundance of alternative splice products is observed between inbred mouse strains and appears to correlate with an intron length polymorphism. This allele of tub is a candidate for a previously reported diet-induced obesity quantitative trait locus on mouse chromosome 7.
journal_name
Celljournal_title
Cellauthors
Kleyn PW,Fan W,Kovats SG,Lee JJ,Pulido JC,Wu Y,Berkemeier LR,Misumi DJ,Holmgren L,Charlat O,Woolf EA,Tayber O,Brody T,Shu P,Hawkins F,Kennedy B,Baldini L,Ebeling C,Alperin GD,Deeds J,Lakey ND,Culpepper J,Chendoi
10.1016/s0092-8674(00)81104-6subject
Has Abstractpub_date
1996-04-19 00:00:00pages
281-90issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81104-6journal_volume
85pub_type
杂志文章相关文献
CELL文献大全abstract::Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.12.019
更新日期:2017-01-12 00:00:00
abstract::We here attempt to achieve an integrated understanding of the structure and dynamics of a number of higher-order assemblies, including amyloids, various kinds of signalosomes, and cellular granules. We propose that the synergy between folded domains, linear motifs, and intrinsically disordered regions regulates the fo...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.05.004
更新日期:2016-05-19 00:00:00
abstract::We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(91)90098-j
更新日期:1991-05-17 00:00:00
abstract::Suppressor of Hairless [Su(H)]/Lag-1/RBP-Jkappa/CBF1 is the only known transducing transcription factor for Notch receptor signaling. Here, we show that Su(H) has three distinct functions in the development of external mechanosensory organs in Drosophila: Notch-dependent transcriptional activation and a novel auto-rep...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00198-7
更新日期:2000-12-08 00:00:00
abstract::The "protective protein" is a glycoprotein that associates with lysosomal beta-galactosidase and neuraminidase and is deficient in the autosomal recessive disorder galactosialidosis. We have isolated the cDNA encoding human "protective protein". The clone recognizes a 2 kb mRNA in normal cells that is not evident in f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(88)90999-3
更新日期:1988-09-09 00:00:00
abstract::Signaling by ErbB receptors requires the activation of their cytoplasmic kinase domains, which is initiated by ligand binding to the receptor ectodomains. Cytoplasmic factors contributing to the activation are unknown. Here we identify members of the cytohesin protein family as such factors. Cytohesin inhibition decre...
journal_title:Cell
pub_type: 杂志文章,收录出版
doi:10.1016/j.cell.2010.09.011
更新日期:2010-10-15 00:00:00
abstract::The 10Sa RNA, encoded by the E. coli ssrA gene, appears to modulate action of some DNA-binding proteins. When ssrA is inactivated, lacZ expression from the lac operon, as well as galK from a gal operon fused to a phage lambda promoter, is reduced from that observed in bacteria wild-type for ssrA. These differences are...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90164-7
更新日期:1995-10-20 00:00:00
abstract::Bacteria can respond to adverse environmental conditions by drastically reducing or even ceasing metabolic activity. They must then determine that conditions have improved before exiting dormancy, and one indication of such a change is the growth of other bacteria in the local environment. Growing bacteria release mur...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.08.039
更新日期:2008-10-31 00:00:00
abstract::Ethylene regulates many aspects of plant growth and development. In the presence of ethylene, the C terminus of EIN2 (EIN2C) translocates into the nucleus and activates transcription. Li et al. and Merchante et al. show that EIN2C also regulates translation through an interaction with the 3' UTRs of transcripts. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.10.032
更新日期:2015-10-22 00:00:00
abstract::The mechanism by which cells decide to skip mitosis to become polyploid is largely undefined. Here we used a high-content image-based screen to identify small-molecule probes that induce polyploidization of megakaryocytic leukemia cells and serve as perturbagens to help understand this process. Our study implicates fi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.06.032
更新日期:2012-08-03 00:00:00
abstract::Secreted proteins of the Wnt family have profound organizing roles during animal development and are transduced via the activities of the Frizzled (Fz) class of transmembrane receptors and the TCF/LEF/Pangolin class of transcription factors. beta-catenins, including Drosophila Armadillo (Arm), link activation of Fz at...
journal_title:Cell
pub_type: 杂志文章,收录出版
doi:10.1016/s0092-8674(02)01037-1
更新日期:2002-10-18 00:00:00
abstract::Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and lab...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.064
更新日期:2016-06-30 00:00:00
abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90016-3
更新日期:1979-02-01 00:00:00
abstract::Senescence in Podospora anserina has long been shown to be under cytoplasmic control. Comparison of DNAs isolated from young and senescent cultures made it possible to detect the presence, in senescent cultures only, of a specific DNA (SEN-DNA). This DNA consists of repeated sequences arranged in a multimeric set of c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90126-9
更新日期:1980-08-01 00:00:00
abstract::During cell division, newly replicated DNA is actively segregated to the daughter cells. In most bacteria, this process involves the DNA-binding protein ParB, which condenses the centromeric regions of sister DNA molecules into kinetochore-like structures that recruit the DNA partition ATPase ParA and the prokaroytic ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.11.015
更新日期:2019-12-12 00:00:00
abstract::Oncogenic activation of RAS genes via point mutations occurs in 20%-30% of human cancers. The development of effective RAS inhibitors has been challenging, necessitating new approaches to inhibit this oncogenic protein. Functional studies have shown that the switch region of RAS interacts with a large number of effect...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.03.045
更新日期:2016-04-21 00:00:00
abstract::Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25 degrees C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90155-0
更新日期:1981-02-01 00:00:00
abstract::Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.10.011
更新日期:2016-11-03 00:00:00
abstract::The brain produces two brain-derived neurotrophic factor (BDNF) transcripts, with either short or long 3' untranslated regions (3' UTRs). The physiological significance of the two forms of mRNAs encoding the same protein is unknown. Here, we show that the short and long 3' UTR BDNF mRNAs are involved in different cell...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.05.045
更新日期:2008-07-11 00:00:00
abstract::The p53 tumor suppressor gene is commonly altered in human tumors, predominantly through missense mutations that result in accumulation of mutant p53 protein. These mutations may confer dominant-negative or gain-of-function properties to p53. To ascertain the physiological effects of p53 point mutation, the structural...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.004
更新日期:2004-12-17 00:00:00
abstract::Here we characterize the T-cell-activating protein (TAP), an Ly-6 gene product involved in T cell activation, as a glycoprotein with a molecular weight of 10-12 kd under nonreducing conditions and 15-18 kd under reducing ones. Two of the three bands that are precipitated from metabolically labeled cells are expressed ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90593-3
更新日期:1986-11-07 00:00:00
abstract::Embryonic stem cells (ESCs) repress the expression of exogenous proviruses and endogenous retroviruses (ERVs). Here, we systematically dissected the cellular factors involved in provirus repression in embryonic carcinomas (ECs) and ESCs by a genome-wide siRNA screen. Histone chaperones (Chaf1a/b), sumoylation factors ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.08.037
更新日期:2015-09-24 00:00:00
abstract::Two-component signal transduction systems are the predominant means by which bacteria sense and respond to environmental stimuli. Bacteria often employ tens or hundreds of these paralogous signaling systems, comprised of histidine kinases (HKs) and their cognate response regulators (RRs). Faithful transmission of info...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2008.04.040
更新日期:2008-06-13 00:00:00
abstract::Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphory...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81840-1
更新日期:1997-01-24 00:00:00
abstract::We employed gene targeting to study H2AX, a histone variant phosphorylated in chromatin surrounding DNA double-strand breaks. Mice deficient for both H2AX and p53 (H(delta/delta)P(-/-)) rapidly developed immature T and B lymphomas and solid tumors. Moreover, H2AX haploinsufficiency caused genomic instability in normal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00566-x
更新日期:2003-08-08 00:00:00
abstract::Solute carrier (SLC) membrane transport proteins control essential physiological functions, including nutrient uptake, ion transport, and waste removal. SLCs interact with several important drugs, and a quarter of the more than 400 SLC genes are associated with human diseases. Yet, compared to other gene families of s...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2015.07.022
更新日期:2015-07-30 00:00:00
abstract::On each side of the midline of the Drosophila CNS, axons are organized into a series of parallel pathways. Here we show that the midline repellent Slit, previously identified as a short-range signal that regulates midline crossing, also functions at long range to pattern these longitudinal pathways. In this long-range...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00207-5
更新日期:2000-12-22 00:00:00
abstract::The assembly of new histones into nucleosomes and the segregation of old histones during replication were investigated using a density gradient, sedimentation equilibrium analysis of histones labeled in vivo with dense amino acids. After a 1 hr pulse of dense amino acids and 3H-lysine, nucleosomes were isolated from c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90282-3
更新日期:1977-11-01 00:00:00
abstract::During adaptive angiogenesis, a key process in the etiology and treatment of cancer and obesity, the vasculature changes to meet the metabolic needs of its target tissues. Although the cues governing vascular remodeling are not fully understood, target-derived signals are generally believed to underlie this process. H...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.12.008
更新日期:2014-01-16 00:00:00
abstract::S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81084-3
更新日期:1996-04-05 00:00:00