Abstract：
:S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic fusion event can be assayed in vitro. It requires Sec17p and Sec18p, the homologs of the mammalian alpha-SNAP and NSF, which cooperate in multiple steps of membrane trafficking. We now report that Sec17p, Sec18p, and ATP are only needed for an early stage of the reaction that results in Sec17p release. Sec17p and Sec18p actions precede, and are needed for, the step employing the Ras-like GTPase Ypt7p. Sec18p-driven release of Sec17p can even precede vacuole docking, as it can occur prior to mixing of vacuoles and is insensitive to vacuole concentration. Sec17p and Sec18p thus may function in a predocking stage of the reaction, rather than in bilayer fusion per se.
journal_name
Celljournal_title
Cellauthors
Mayer A,Wickner W,Haas Adoi
10.1016/s0092-8674(00)81084-3subject
Has Abstractpub_date
1996-04-05 00:00:00pages
83-94issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)81084-3journal_volume
85pub_type
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