Abstract:
:Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25 degrees C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages isolated from heat-shocked and non--heat-shocked Kc cells is unaltered when analyzed by in vitro translation in the rabbit reticulocyte lysate. In contrast, homologous cell-free translation systems obtained from Kc cells effectively discriminate between the in vitro translation of normal cellular messages and heat-shock--specific mRNAs. In particular, a cell-free system from heat-shocked Drosophila Kc cells almost completely shuts down the translation of 25 degrees C messenger RNA species, whereas the translatability of heat-shock--specific messenger RNA appears to be unaffected.
journal_name
Celljournal_title
Cellauthors
Krüger C,Benecke BJdoi
10.1016/0092-8674(81)90155-0subject
Has Abstractpub_date
1981-02-01 00:00:00pages
595-603issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(81)90155-0journal_volume
23pub_type
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