In vitro translation of Drosophila heat-shock and non--heat-shock mRNAs in heterologous and homologous cell-free systems.

Abstract:

:Upon heat shock, Drosophila Kc cells still contain normal cellular messenger RNAs in the cytoplasm. The distribution of these 25 degrees C mRNAs between polysomes and the postpolysomal fraction of heat-shocked cells appears unaltered as compared with control cells. The translatability of these normal cellular messages isolated from heat-shocked and non--heat-shocked Kc cells is unaltered when analyzed by in vitro translation in the rabbit reticulocyte lysate. In contrast, homologous cell-free translation systems obtained from Kc cells effectively discriminate between the in vitro translation of normal cellular messages and heat-shock--specific mRNAs. In particular, a cell-free system from heat-shocked Drosophila Kc cells almost completely shuts down the translation of 25 degrees C messenger RNA species, whereas the translatability of heat-shock--specific messenger RNA appears to be unaffected.

journal_name

Cell

journal_title

Cell

authors

Krüger C,Benecke BJ

doi

10.1016/0092-8674(81)90155-0

subject

Has Abstract

pub_date

1981-02-01 00:00:00

pages

595-603

issue

2

eissn

0092-8674

issn

1097-4172

pii

0092-8674(81)90155-0

journal_volume

23

pub_type

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