Abstract：
:Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PAPERCLIP.
journal_name
Celljournal_title
Cellauthors
Antonovsky N,Gleizer S,Noor E,Zohar Y,Herz E,Barenholz U,Zelcbuch L,Amram S,Wides A,Tepper N,Davidi D,Bar-On Y,Bareia T,Wernick DG,Shani I,Malitsky S,Jona G,Bar-Even A,Milo Rdoi
10.1016/j.cell.2016.05.064subject
Has Abstractpub_date
2016-06-30 00:00:00pages
115-25issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(16)30668-7journal_volume
166pub_type
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