Abstract：
:Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from the cytoplasmic one by proteolytic cleavage into 53 and 17 kd polypeptides that remain associated during isolation. DNA sequence determination reveals four tandemly arrayed 90 amino acid regions of homology that probably represent poly(A)-binding domains. A 55 residue A-rich region upstream of the initiator methionine codon in the mRNA shows an affinity for poly(A)-binding protein comparable to that of poly(A)180-220, raising the possibility of feedback regulation of translation.
journal_name
Celljournal_title
Cellauthors
Sachs AB,Bond MW,Kornberg RDdoi
10.1016/0092-8674(86)90557-xsubject
Has Abstractpub_date
1986-06-20 00:00:00pages
827-35issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(86)90557-Xjournal_volume
45pub_type
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