The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration.


:The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may have an active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptor-like functions of ephrinB2 in mice, we replaced the endogenous gene by cDNAs encoding either carboxyterminally truncated (ephrinB2(DeltaC)) or, as a control, full-length ligand (ephrinB2(WT)). While homozygous ephrinB2(WT/WT) animals were viable and fertile, loss of the ephrinB2 cytoplasmic domain resulted in midgestation lethality similar to ephrinB2 null mutants (ephrinB2(KO)). The truncated ligand was sufficient to restore guidance of migrating cranial neural crest cells, but ephrinB2(DeltaC/DeltaC) embryos showed defects in vasculogenesis and angiogenesis very similar to those observed in ephrinB2(KO/KO) animals. Our results indicate distinct requirements of functions mediated by the ephrinB carboxyterminus for developmental processes in the vertebrate embryo.






Adams RH,Diella F,Hennig S,Helmbacher F,Deutsch U,Klein R




Has Abstract


2001-01-12 00:00:00
















  • The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1.

    abstract::The anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 controls progression to S phase and mitosis by stabilizing key APC/C ubiquitination substrates, including cyclin A. Examining Emi1 binding proteins, we identified the Evi5 oncogene as a regulator of Emi1 accumulation. Evi5 antagonizes SCF(betaTrCP)-depend...


    pub_type: 杂志文章


    authors: Eldridge AG,Loktev AV,Hansen DV,Verschuren EW,Reimann JD,Jackson PK

    更新日期:2006-01-27 00:00:00

  • TGF-beta signaling specifies axons during brain development.

    abstract::In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...


    pub_type: 杂志文章


    authors: Yi JJ,Barnes AP,Hand R,Polleux F,Ehlers MD

    更新日期:2010-07-09 00:00:00

  • Planar polarity and tissue morphogenesis.

    abstract::Planar polarity is a global, tissue-level phenomenon that coordinates cell behavior in a two-dimensional plane. The Frizzled/planar cell polarity (PCP) and anterior-posterior (AP) patterning systems for planar polarity operate in a variety of cell types and provide direction to cells with different morphologies and be...


    pub_type: 杂志文章,评审


    authors: Zallen JA

    更新日期:2007-06-15 00:00:00

  • Mutations of immunoglobulin transmembrane and cytoplasmic domains: effects on intracellular signaling and antigen presentation.

    abstract::The membrane-bound form of immunoglobulin serves as an antigen-specific receptor for B cells mediating signal transduction and antigen presentation. We have developed an assay that reconstitutes both these physiologic responses with respect to the antigen phosphorylcholine. By introducing specific mutations in the hum...


    pub_type: 杂志文章


    authors: Shaw AC,Mitchell RN,Weaver YK,Campos-Torres J,Abbas AK,Leder P

    更新日期:1990-10-19 00:00:00

  • Ku86-deficient mice exhibit severe combined immunodeficiency and defective processing of V(D)J recombination intermediates.

    abstract::Ku is a heterodimeric DNA end binding complex composed of 70 and 86 kDa subunits. Here, we show that Ku86 is essential for normal V(D)J recombination in vivo, as Ku86-deficient mice are severely defective for formation of coding joints. Unlike severe combined immunodeficient (scid) mice, Ku86-deficient mice are also d...


    pub_type: 杂志文章


    authors: Zhu C,Bogue MA,Lim DS,Hasty P,Roth DB

    更新日期:1996-08-09 00:00:00

  • Isolation and characterization of a serine esterase from cytolytic T cell granules.

    abstract::Cytotoxic T lymphocytes and lymphocytes with NK-like activity contain a serine esterase activity which has been localized to their cytoplasmic granules by cytochemistry and subcellular fractionation studies. The serine esterase-specific inhibitor 3H-DFP labels two protein species in the granules. The two proteins, ref...


    pub_type: 杂志文章


    authors: Young JD,Leong LG,Liu CC,Damiano A,Wall DA,Cohn ZA

    更新日期:1986-10-24 00:00:00

  • Variant T47D human breast cancer cells with high progesterone-receptor levels despite estrogen and antiestrogen resistance.

    abstract::In target tissues for estrogen, including breast cancer cells, the synthesis of progesterone receptors (PRs) is controlled by estradiol acting through estrogen receptors (ERs). We describe studies with T47D human breast cancer cells, whose PRs are not regulated by estradiol, though present in extraordinary amounts (30...


    pub_type: 杂志文章


    authors: Horwitz KB,Mockus MB,Lessey BA

    更新日期:1982-03-01 00:00:00

  • Nerve growth factor is an autocrine survival factor for memory B lymphocytes.

    abstract::Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused...


    pub_type: 杂志文章


    authors: Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E

    更新日期:1996-05-03 00:00:00

  • Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment.

    abstract::The Reelin ligand regulates a Dab1-dependent signaling pathway required for brain lamination and normal dendritogenesis, but the specific mechanisms underlying these actions remain unclear. We find that Stk25, a modifier of Reelin-Dab1 signaling, regulates Golgi morphology and neuronal polarization as part of an LKB1-...


    pub_type: 杂志文章


    authors: Matsuki T,Matthews RT,Cooper JA,van der Brug MP,Cookson MR,Hardy JA,Olson EC,Howell BW

    更新日期:2010-11-24 00:00:00

  • Immunogenic structure of the influenza virus hemagglutinin.

    abstract::We chemically synthesized 20 peptides corresponding to 75% of the HA1 molecule of the influenza virus. Antibodies to the majority (18) of these peptides were capable of reacting with the hemagglutinin molecule. These 18 peptides are not confined to the known antigenic determinants of the hemagglutinin molecule, but ra...


    pub_type: 杂志文章


    authors: Green N,Alexander H,Olson A,Alexander S,Shinnick TM,Sutcliffe JG,Lerner RA

    更新日期:1982-03-01 00:00:00

  • Conversion of Bcl-2 from protector to killer by interaction with nuclear orphan receptor Nur77/TR3.

    abstract::The Bcl-2 family proteins are key regulators of apoptosis in human diseases and cancers. Though known to block apoptosis, Bcl-2 promotes cell death through an undefined mechanism. Here, we show that Bcl-2 interacts with orphan nuclear receptor Nur77 (also known as TR3), which is required for cancer cell apoptosis indu...


    pub_type: 杂志文章


    authors: Lin B,Kolluri SK,Lin F,Liu W,Han YH,Cao X,Dawson MI,Reed JC,Zhang XK

    更新日期:2004-02-20 00:00:00

  • Mini-P1 plasmid replication: the autoregulation-sequestration paradox.

    abstract::It has been proposed that the initiator protein RepA is rate limiting for mini-P1 plasmid replication, and that the role of the plasmid copy number control locus is to sequester the initiator and thus reduce replication. This proposal appears inconsistent with the observation that RepA is autoregulated, since the prot...


    pub_type: 杂志文章


    authors: Chattoraj DK,Mason RJ,Wickner SH

    更新日期:1988-02-26 00:00:00

  • An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6.

    abstract::The mitochondrial MURF4 gene of T. brucei has pronounced G versus C strand bias and heterogeneously sized transcripts, characteristic of genes encoding extensively edited transcripts. We find that MURF4 transcripts of T. brucei are extensively edited throughout by the addition and deletion of numerous uridines, creati...


    pub_type: 杂志文章


    authors: Bhat GJ,Koslowsky DJ,Feagin JE,Smiley BL,Stuart K

    更新日期:1990-06-01 00:00:00

  • Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells.

    abstract::We mutated, by gene targeting, the endogenous hypoxanthine phosphoribosyl transferase (HPRT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (neor) gene was introduced into an exon of a cloned fragment of the Hprt gene and used to transfect ES cells. Among the G418r col...


    pub_type: 杂志文章


    authors: Thomas KR,Capecchi MR

    更新日期:1987-11-06 00:00:00

  • Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.

    abstract::The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA bindin...


    pub_type: 杂志文章


    authors: Tanaka M,Lai JS,Herr W

    更新日期:1992-02-21 00:00:00

  • A Smad transcriptional corepressor.

    abstract::Following TGFbeta receptor-mediated phosphorylation and association with Smad4, Smad2 moves into the nucleus, binds to target promoters in association with DNA-binding cofactors, and recruits coactivators such as p300/CBP to activate transcription. We identified the homeodomain protein TGIF as a Smad2-binding protein ...


    pub_type: 杂志文章


    authors: Wotton D,Lo RS,Lee S,Massagué J

    更新日期:1999-04-02 00:00:00

  • The Extracellular Surface of the GLP-1 Receptor Is a Molecular Trigger for Biased Agonism.

    abstract::Ligand-directed signal bias offers opportunities for sculpting molecular events, with the promise of better, safer therapeutics. Critical to the exploitation of signal bias is an understanding of the molecular events coupling ligand binding to intracellular signaling. Activation of class B G protein-coupled receptors ...


    pub_type: 杂志文章


    authors: Wootten D,Reynolds CA,Smith KJ,Mobarec JC,Koole C,Savage EE,Pabreja K,Simms J,Sridhar R,Furness SGB,Liu M,Thompson PE,Miller LJ,Christopoulos A,Sexton PM

    更新日期:2016-06-16 00:00:00

  • Mechanism of transcriptional silencing in yeast.

    abstract::Transcriptional silencing is a phenomenon in which the transcription of a gene by RNA polymerase II or III is repressed or not, dependent only on the gene's chromosomal location. Two prevailing models exist for silencing: (1) steric hindrance in silenced chromatin inhibits the binding of upstream activator proteins or...


    pub_type: 杂志文章


    authors: Chen L,Widom J

    更新日期:2005-01-14 00:00:00

  • The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product.

    abstract::Mutations in the single-minded (sim) gene of Drosophila result in the loss of the precursor cells giving rise to the midline cells of the embryonic central nervous system. We have examined the structure of the sim product by sequencing a sim cDNA clone, and have also determined the subcellular localization of the prot...


    pub_type: 杂志文章


    authors: Crews ST,Thomas JB,Goodman CS

    更新日期:1988-01-15 00:00:00

  • Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space.

    abstract::The exchange of metabolites between the mitochondrial matrix and the cytosol depends on β-barrel channels in the outer membrane and α-helical carrier proteins in the inner membrane. The essential translocase of the inner membrane (TIM) chaperones escort these proteins through the intermembrane space, but the structura...


    pub_type: 杂志文章


    authors: Weinhäupl K,Lindau C,Hessel A,Wang Y,Schütze C,Jores T,Melchionda L,Schönfisch B,Kalbacher H,Bersch B,Rapaport D,Brennich M,Lindorff-Larsen K,Wiedemann N,Schanda P

    更新日期:2018-11-15 00:00:00

  • Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.

    abstract::Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-p...


    pub_type: 杂志文章


    authors: Pierschbacher MD,Hayman EG,Ruoslahti E

    更新日期:1981-10-01 00:00:00

  • A Call for Systematic Research on Solute Carriers.

    abstract::Solute carrier (SLC) membrane transport proteins control essential physiological functions, including nutrient uptake, ion transport, and waste removal. SLCs interact with several important drugs, and a quarter of the more than 400 SLC genes are associated with human diseases. Yet, compared to other gene families of s...


    pub_type: 杂志文章,评审


    authors: César-Razquin A,Snijder B,Frappier-Brinton T,Isserlin R,Gyimesi G,Bai X,Reithmeier RA,Hepworth D,Hediger MA,Edwards AM,Superti-Furga G

    更新日期:2015-07-30 00:00:00

  • Analysis of a mutant strain of human fibroblasts with a defect in the internalization of receptor-bound low density lipoprotein.

    abstract::A new type of mutation that affects a discrete step in the process of adsorptive endocytosis has been identified in one of 22 strains of fibroblasts derived from subjects with the clinical phenotype of homozygous familial hypercholesterolemia (FH). In this unique mutant strain (derived from subject J.D.), the cell sur...


    pub_type: 杂志文章


    authors: Brown MS,Goldstein JL

    更新日期:1976-12-01 00:00:00

  • Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos.

    abstract::In a 4-cell stage C. elegans embryo, signaling by the P2 blastomere induces anterior-posterior polarity in the adjacent EMS blastomere, leading to endoderm formation. We have taken genetic and reverse genetic approaches toward understanding the molecular basis for this induction. These studies have identified a set of...


    pub_type: 杂志文章


    authors: Rocheleau CE,Downs WD,Lin R,Wittmann C,Bei Y,Cha YH,Ali M,Priess JR,Mello CC

    更新日期:1997-08-22 00:00:00

  • Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells.

    abstract::Despite the obvious importance of epithelial stem cells in tissue homeostasis and tumorigenesis, little is known about their specific location or biological characteristics. Using 3H-thymidine labeling, we have identified a subpopulation of corneal epithelial basal cells, located in the peripheral cornea in a region c...


    pub_type: 杂志文章


    authors: Cotsarelis G,Cheng SZ,Dong G,Sun TT,Lavker RM

    更新日期:1989-04-21 00:00:00

  • Gene organization and recombinational hotspots in the murine major histocompatibility complex.

    abstract::By chromosome walking in the major histocompatibility complex (MHC) of the BALB/c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events we...


    pub_type: 杂志文章


    authors: Steinmetz M,Stephan D,Fischer Lindahl K

    更新日期:1986-03-28 00:00:00

  • Glucocorticoid receptor confers resistance to antiandrogens by bypassing androgen receptor blockade.

    abstract::The treatment of advanced prostate cancer has been transformed by novel antiandrogen therapies such as enzalutamide. Here, we identify induction of glucocorticoid receptor (GR) expression as a common feature of drug-resistant tumors in a credentialed preclinical model, a finding also confirmed in patient samples. GR s...


    pub_type: 杂志文章


    authors: Arora VK,Schenkein E,Murali R,Subudhi SK,Wongvipat J,Balbas MD,Shah N,Cai L,Efstathiou E,Logothetis C,Zheng D,Sawyers CL

    更新日期:2013-12-05 00:00:00

  • Organ-level quorum sensing directs regeneration in hair stem cell populations.

    abstract::Coordinated organ behavior is crucial for an effective response to environmental stimuli. By studying regeneration of hair follicles in response to patterned hair plucking, we demonstrate that organ-level quorum sensing allows coordinated responses to skin injury. Plucking hair at different densities leads to a regene...


    pub_type: 杂志文章


    authors: Chen CC,Wang L,Plikus MV,Jiang TX,Murray PJ,Ramos R,Guerrero-Juarez CF,Hughes MW,Lee OK,Shi S,Widelitz RB,Lander AD,Chuong CM

    更新日期:2015-04-09 00:00:00

  • Decoding the Genomics of Abdominal Aortic Aneurysm.

    abstract::A key aspect of genomic medicine is to make individualized clinical decisions from personal genomes. We developed a machine-learning framework to integrate personal genomes and electronic health record (EHR) data and used this framework to study abdominal aortic aneurysm (AAA), a prevalent irreversible cardiovascular ...


    pub_type: 杂志文章


    authors: Li J,Pan C,Zhang S,Spin JM,Deng A,Leung LLK,Dalman RL,Tsao PS,Snyder M

    更新日期:2018-09-06 00:00:00

  • DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

    abstract::DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...


    pub_type: 杂志文章


    authors: Bishop DK,Park D,Xu L,Kleckner N

    更新日期:1992-05-01 00:00:00