Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery.


:Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culminates in the phosphorylation of the BCL-2 family member BAD, thereby suppressing apoptosis and promoting cell survival. Akt phosphorylates BAD in vitro and in vivo, and blocks the BAD-induced death of primary neurons in a site-specific manner. These findings define a mechanism by which growth factors directly inactivate a critical component of the cell-intrinsic death machinery.






Datta SR,Dudek H,Tao X,Masters S,Fu H,Gotoh Y,Greenberg ME




Has Abstract


1997-10-17 00:00:00
















  • The use of promoter fusions in Drosophila genetics: isolation of mutations affecting the heat shock response.

    abstract::We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) ...


    pub_type: 杂志文章


    authors: Bonner JJ,Parks C,Parker-Thornburg J,Mortin MA,Pelham HR

    更新日期:1984-07-01 00:00:00

  • CDK7 inhibition suppresses super-enhancer-linked oncogenic transcription in MYCN-driven cancer.

    abstract::The MYC oncoproteins are thought to stimulate tumor cell growth and proliferation through amplification of gene transcription, a mechanism that has thwarted most efforts to inhibit MYC function as potential cancer therapy. Using a covalent inhibitor of cyclin-dependent kinase 7 (CDK7) to disrupt the transcription of a...


    pub_type: 杂志文章


    authors: Chipumuro E,Marco E,Christensen CL,Kwiatkowski N,Zhang T,Hatheway CM,Abraham BJ,Sharma B,Yeung C,Altabef A,Perez-Atayde A,Wong KK,Yuan GC,Gray NS,Young RA,George RE

    更新日期:2014-11-20 00:00:00

  • Gradients of the Drosophila Chinmo BTB-zinc finger protein govern neuronal temporal identity.

    abstract::Many neural progenitors, including Drosophila mushroom body (MB) and projection neuron (PN) neuroblasts, sequentially give rise to different subtypes of neurons throughout development. We identified a novel BTB-zinc finger protein, named Chinmo (Chronologically inappropriate morphogenesis), that governs neuronal tempo...


    pub_type: 杂志文章


    authors: Zhu S,Lin S,Kao CF,Awasaki T,Chiang AS,Lee T

    更新日期:2006-10-20 00:00:00

  • Proteolytic processing of histone H3 in chromatin: a physiologically regulated event in Tetrahymena micronuclei.

    abstract::Micronuclei of Tetrahymena thermophila contain two electrophoretically distinct forms of histone H3. The slower migrating micronuclear species, H3S, is indistinguishable from the macronuclear H3 by electrophoretic analyses in three gel systems and by partial proteolytic peptide mapping. The faster species, H3F, is uni...


    pub_type: 杂志文章


    authors: Allis CD,Bowen JK,Abraham GN,Glover CV,Gorovsky MA

    更新日期:1980-05-01 00:00:00

  • Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis.

    abstract::The cpd mutation localized by T-DNA tagging on Arabidopsis chromosome 5-14.3 inhibits cell elongation controlled by the ecdysone-like brassinosteroid hormone brassinolide. The cpd mutant displays de-etiolation and derepression of light-induced genes in the dark, as well as dwarfism, male sterility, and activation of s...


    pub_type: 杂志文章


    authors: Szekeres M,Németh K,Koncz-Kálmán Z,Mathur J,Kauschmann A,Altmann T,Rédei GP,Nagy F,Schell J,Koncz C

    更新日期:1996-04-19 00:00:00

  • A Biogenesis Step Upstream of Microprocessor Controls miR-17∼92 Expression.

    abstract::The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem c...


    pub_type: 杂志文章


    authors: Du P,Wang L,Sliz P,Gregory RI

    更新日期:2015-08-13 00:00:00

  • Function of E. coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing.

    abstract::The sigma factor sigma 70 of E. coli RNA polymerase acts not only in initiation, but also at an early stage of elongation to induce a transcription pause, and simultaneously to allow the phage lambda gene Q transcription antiterminator to act. We identify the signal in DNA that induces early pausing to be a version of...


    pub_type: 杂志文章


    authors: Ring BZ,Yarnell WS,Roberts JW

    更新日期:1996-08-09 00:00:00

  • A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.

    abstract::Two cDNAs were isolated whose dimerized products bind specifically to a DNA sequence, kappa E2, located in the immunoglobulin kappa chain enhancer. Both cDNAs share a region of extensive identity to the Drosophila daughterless gene and obvious similarity to a segment in three myc proteins, MyoD, and members of the Dro...


    pub_type: 杂志文章


    authors: Murre C,McCaw PS,Baltimore D

    更新日期:1989-03-10 00:00:00

  • The ATAC acetyltransferase complex coordinates MAP kinases to regulate JNK target genes.

    abstract::In response to extracellular cues, signal transduction activates downstream transcription factors like c-Jun to induce expression of target genes. We demonstrate that the ATAC (Ada two A containing) histone acetyltransferase (HAT) complex serves as a transcriptional cofactor for c-Jun at the Jun N-terminal kinase (JNK...


    pub_type: 杂志文章


    authors: Suganuma T,Mushegian A,Swanson SK,Abmayr SM,Florens L,Washburn MP,Workman JL

    更新日期:2010-09-03 00:00:00

  • The path to perdition is paved with protons.

    abstract::Recent studies are beginning to shed light on the mechanism of ribosome-catalyzed peptide bond formation. ...


    pub_type: 杂志文章,评审


    authors: Green R,Lorsch JR

    更新日期:2002-09-20 00:00:00

  • Isolation and characterization of sigma(70)-retaining transcription elongation complexes from Escherichia coli.

    abstract::sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular ...


    pub_type: 杂志文章


    authors: Bar-Nahum G,Nudler E

    更新日期:2001-08-24 00:00:00

  • Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.

    abstract::Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking en...


    pub_type: 杂志文章


    authors: Qi LS,Larson MH,Gilbert LA,Doudna JA,Weissman JS,Arkin AP,Lim WA

    更新日期:2013-02-28 00:00:00

  • Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

    abstract::Post-translational modifications of histone proteins and exchanges of histone variants of chromatin are central to the regulation of nearly all DNA-templated biological processes. However, the degree and variability of chromatin modifications in specific human immune cells remain largely unknown. Here, we employ a hig...


    pub_type: 杂志文章


    authors: Cheung P,Vallania F,Warsinske HC,Donato M,Schaffert S,Chang SE,Dvorak M,Dekker CL,Davis MM,Utz PJ,Khatri P,Kuo AJ

    更新日期:2018-05-31 00:00:00

  • Mechanism of replication-coupled DNA interstrand crosslink repair.

    abstract::DNA interstrand crosslinks (ICLs) are toxic DNA lesions whose repair occurs in the S phase of metazoans via an unknown mechanism. Here, we describe a cell-free system based on Xenopus egg extracts that supports ICL repair. During DNA replication of a plasmid containing a site-specific ICL, two replication forks conver...


    pub_type: 杂志文章


    authors: Räschle M,Knipscheer P,Enoiu M,Angelov T,Sun J,Griffith JD,Ellenberger TE,Schärer OD,Walter JC

    更新日期:2008-09-19 00:00:00

  • Representation of the glomerular olfactory map in the Drosophila brain.

    abstract::We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...


    pub_type: 杂志文章


    authors: Marin EC,Jefferis GS,Komiyama T,Zhu H,Luo L

    更新日期:2002-04-19 00:00:00

  • Screening Human Embryos for Polygenic Traits Has Limited Utility.

    abstract::The increasing proportion of variance in human complex traits explained by polygenic scores, along with progress in preimplantation genetic diagnosis, suggests the possibility of screening embryos for traits such as height or cognitive ability. However, the expected outcomes of embryo screening are unclear, which unde...


    pub_type: 杂志文章


    authors: Karavani E,Zuk O,Zeevi D,Barzilai N,Stefanis NC,Hatzimanolis A,Smyrnis N,Avramopoulos D,Kruglyak L,Atzmon G,Lam M,Lencz T,Carmi S

    更新日期:2019-11-27 00:00:00

  • The synthesis and distribution of desmin and vimentin during myogenesis in vitro.

    abstract::Electrophoretic and autoradiographic analyses of the incorporation of 35S--methionine into newly synthesized proteins during myogenesis reveal that presumptive chicken myoblasts synthesize primarily one intermediate filament protein: vimentin. Desmin synthesis is initiated at the onset of fusion. Synthesis rates of bo...


    pub_type: 杂志文章


    authors: Gard DL,Lazarides E

    更新日期:1980-01-01 00:00:00

  • Mouse development and cell proliferation in the absence of D-cyclins.

    abstract::D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. We tested the requirement for D-cyclins in mouse development and in proliferation by generating mice lacking all D-cyclins. We found that these cyclin D1(-/-)D2(-/-)D3(-/-) mice develop u...


    pub_type: 杂志文章


    authors: Kozar K,Ciemerych MA,Rebel VI,Shigematsu H,Zagozdzon A,Sicinska E,Geng Y,Yu Q,Bhattacharya S,Bronson RT,Akashi K,Sicinski P

    更新日期:2004-08-20 00:00:00

  • New hope for a microRNA therapy for liver cancer.

    abstract::The loss of expression of particular microRNAs can contribute to tumorigenesis. Kota et al. (2009) now explore in a mouse model a promising new approach for the treatment of liver cancer-re-establishing the expression of an miRNA using a viral vector. ...


    pub_type: 评论,杂志文章


    authors: Rossi JJ

    更新日期:2009-06-12 00:00:00

  • Location of sequences on the adenovirus genome coding for the 5.5S RNA.

    abstract::The origin of a low molecular weight virus-associated RNA (VA-RNA) was mapped by hybridization of VA-RNA to specific fragments of adenovirus type 2 DNA, obtained after cleavage with three different restriction endonucleases. VA-RNA was found to hybridize exculsively to the l-strand [strand with low buoyant density in ...


    pub_type: 杂志文章


    authors: Pettersson U,Philipson L

    更新日期:1975-09-01 00:00:00

  • Specified neural progenitors sort to form sharp domains after noisy Shh signaling.

    abstract::Sharply delineated domains of cell types arise in developing tissues under instruction of inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. The translation of a morphogen gradient into discrete spatial domains relies on precise signal responses at stable cell positio...


    pub_type: 杂志文章


    authors: Xiong F,Tentner AR,Huang P,Gelas A,Mosaliganti KR,Souhait L,Rannou N,Swinburne IA,Obholzer ND,Cowgill PD,Schier AF,Megason SG

    更新日期:2013-04-25 00:00:00

  • Protein kinase C phosphorylation at Thr 654 of the unoccupied EGF receptor and EGF binding regulate functional receptor loss by independent mechanisms.

    abstract::To test the functional consequence of phosphorylation of the EGF receptor at Thr 654 by protein kinase C, the normal Thr 654 human EGF receptor cDNA or a mutant encoding an Ala 654 were expressed in heterologous cells. In cell lines expressing both the Thr 654 and Ala 654 receptors, functional cell-surface Thr 654 rec...


    pub_type: 杂志文章


    authors: Lin CR,Chen WS,Lazar CS,Carpenter CD,Gill GN,Evans RM,Rosenfeld MG

    更新日期:1986-03-28 00:00:00

  • Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family.

    abstract::The mutated gene responsible for the tubby obesity phenotype has been identified by positional cloning. A single base change within a splice donor site results in the incorrect retention of a single intron in the mature tub mRNA transcript. The consequence of this mutation is the substitution of the carboxy-terminal 4...


    pub_type: 杂志文章


    authors: Kleyn PW,Fan W,Kovats SG,Lee JJ,Pulido JC,Wu Y,Berkemeier LR,Misumi DJ,Holmgren L,Charlat O,Woolf EA,Tayber O,Brody T,Shu P,Hawkins F,Kennedy B,Baldini L,Ebeling C,Alperin GD,Deeds J,Lakey ND,Culpepper J,Chen

    更新日期:1996-04-19 00:00:00

  • From microbes to prions the final proof of the prion hypothesis.

    abstract::Much like the "microbe hypothesis" put forth over 150 years ago, the "prion hypothesis" can be definitely proven only if a prion disease is engendered in a natural host from an infectious prion produced in vitro. In this issue of Cell, come very close to accomplishing this goal by producing a prion disease in a natura...


    pub_type: 评论,杂志文章,评审


    authors: Zou WQ,Gambetti P

    更新日期:2005-04-22 00:00:00

  • Physiological and Genetic Adaptations to Diving in Sea Nomads.

    abstract::Understanding the physiology and genetics of human hypoxia tolerance has important medical implications, but this phenomenon has thus far only been investigated in high-altitude human populations. Another system, yet to be explored, is humans who engage in breath-hold diving. The indigenous Bajau people ("Sea Nomads")...


    pub_type: 杂志文章


    authors: Ilardo MA,Moltke I,Korneliussen TS,Cheng J,Stern AJ,Racimo F,de Barros Damgaard P,Sikora M,Seguin-Orlando A,Rasmussen S,van den Munckhof ICL,Ter Horst R,Joosten LAB,Netea MG,Salingkat S,Nielsen R,Willerslev E

    更新日期:2018-04-19 00:00:00

  • DNAase I encapsulated in liposomes can induce neoplastic transformation of Syrian hamster embryo cells in culture.

    abstract::We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...


    pub_type: 杂志文章


    authors: Zajac-Kaye M,Ts'o PO

    更新日期:1984-12-01 00:00:00

  • "Wunder" F-BAR domains: going from pits to vesicles.

    abstract::Clathrin-mediated endocytosis is a key mechanism by which cells take up extracellular cargo. In this issue, Shimada et al. (2007) reveal the mode of action of the F-BAR domain, which deepens the initial membrane pit that forms during clathrin-mediated endocytosis. ...


    pub_type: 评论,杂志文章,评审


    authors: Fütterer K,Machesky LM

    更新日期:2007-05-18 00:00:00

  • A tale of chromatin and transcription in 100 structures.

    abstract::To celebrate a century of X-ray crystallography, I describe how 100 crystal structures influenced chromatin and transcription research. ...


    pub_type: 历史文章,杂志文章,评审


    authors: Cramer P

    更新日期:2014-11-20 00:00:00

  • The nuclear tyrosine kinase c-Abl negatively regulates cell growth.

    abstract::c-Abl is a tyrosine kinase localized primarily in the nucleus. Previous assays for abl function rely on cellular transformation by abl mutants, which are cytoplasmic. Using a conditional overexpression strategy, we have developed a functional assay for c-abl. Overexpression of c-abl inhibits growth by causing cell cyc...


    pub_type: 杂志文章


    authors: Sawyers CL,McLaughlin J,Goga A,Havlik M,Witte O

    更新日期:1994-04-08 00:00:00

  • DNA conformation at the 5' end of the chicken adult beta-globin gene.

    abstract::We have examined the conformation of DNA cloned from the hypersensitive region 5' of the chicken adult beta-globin gene, in order to determine whether the unusual sensitivity to nucleases is related to altered secondary structure of the DNA. The region contains a large number of sites for methylation; we have studied ...


    pub_type: 杂志文章


    authors: Nickol JM,Felsenfeld G

    更新日期:1983-12-01 00:00:00