Abstract:
:Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culminates in the phosphorylation of the BCL-2 family member BAD, thereby suppressing apoptosis and promoting cell survival. Akt phosphorylates BAD in vitro and in vivo, and blocks the BAD-induced death of primary neurons in a site-specific manner. These findings define a mechanism by which growth factors directly inactivate a critical component of the cell-intrinsic death machinery.
journal_name
Celljournal_title
Cellauthors
Datta SR,Dudek H,Tao X,Masters S,Fu H,Gotoh Y,Greenberg MEdoi
10.1016/s0092-8674(00)80405-5subject
Has Abstractpub_date
1997-10-17 00:00:00pages
231-41issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(00)80405-5journal_volume
91pub_type
杂志文章相关文献
CELL文献大全abstract::S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated leve...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90305-8
更新日期:1985-01-01 00:00:00
abstract::Tumor suppressors block the development of cancer and are often lost during tumor development. Papa et al. show that partial loss of normal PTEN tumor suppressor function can be compounded by additional disruption caused by the expression of inactive mutant PTEN protein. This has significant implications for patients ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.04.008
更新日期:2014-04-24 00:00:00
abstract::The V(D)J recombinase subunits Rag-1 and Rag-2 mediate assembly of antigen receptor gene segments. We studied the mechanisms of DNA recognition by Rag-1/Rag-2 using surface plasmon resonance. The critical step for signal recognition is binding of Rag-1 to the nonamer. This is achieved by a region of Rag-1 homologous t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81344-6
更新日期:1996-10-18 00:00:00
abstract::Circadian rhythms control organismal physiology throughout the day. At the cellular level, clock regulation is established by a self-sustained Bmal1-dependent transcriptional oscillator network. However, it is still unclear how different tissues achieve a synchronized rhythmic physiology. That is, do they respond inde...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.05.009
更新日期:2019-05-30 00:00:00
abstract::RORγt(+) Th17 cells are important for mucosal defenses but also contribute to autoimmune disease. They accumulate in the intestine in response to microbiota and produce IL-17 cytokines. Segmented filamentous bacteria (SFB) are Th17-inducing commensals that potentiate autoimmunity in mice. RORγt(+) T cells were induced...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.08.061
更新日期:2015-10-08 00:00:00
abstract::The heterotrimeric Sec61p complex is a major component of the protein-conducting channel of the endoplasmic reticulum (ER) membrane, associating with either ribosomes or the Sec62/63 complex to perform co- and posttranslational transport, respectively. We show by electron microscopy that purified mammalian and yeast S...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81391-4
更新日期:1996-11-15 00:00:00
abstract::New biological tools provide new techniques to probe fundamental biological processes. Here we describe the burgeoning field of proteolysis-targeting chimeras (PROTACs), which are capable of modulating protein concentrations at a post-translational level by co-opting the ubiquitin-proteasome system. We describe the PR...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2019.11.031
更新日期:2020-04-02 00:00:00
abstract::A gene, bcl-3, is found on chromosome 19 adjacent to the breakpoints in the translocation t(14;19)(q32;q13.1), which occurs in some cases of chronic lymphocytic leukemia. Sequence analysis of the human bcl-3 gene predicts a protein containing seven tandem copies of the SWI6/cdc10 motif. This motif was previously ident...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90347-h
更新日期:1990-03-23 00:00:00
abstract::Two papers published in 1984 by the Varshavsky laboratory revealed that the ubiquitin/proteasome pathway is the principal system for degradation of short-lived proteins in mammalian cells, setting the stage for future demonstrations of this pathway's many regulatory roles. This perspective discusses the impact of thos...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/s0092-8674(03)01074-2
更新日期:2004-01-23 00:00:00
abstract::Aging has been associated with a progressive decline of proteostasis, but how this process affects proteome composition remains largely unexplored. Here, we profiled more than 5,000 proteins along the lifespan of the nematode C. elegans. We find that one-third of proteins change in abundance at least 2-fold during agi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.03.032
更新日期:2015-05-07 00:00:00
abstract::Subsets of long-lived, tumor-initiating stem cells often escape cancer therapies. However, sources and mechanisms that generate tumor heterogeneity and drug-resistant cell population are still unfolding. Here, we devise a functional reporter system to lineage trace and/or genetic ablate signaling in TGF-β-activated sq...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.043
更新日期:2015-02-26 00:00:00
abstract::The eye lens contains a structural protein (alpha-crystallin), composed of two homologous primary gene products, alpha A2 and alpha B2. In certain rodents, there is another minor alpha-crystallin polypeptide, alpha Ains, which is identical to alpha A2 except for a 22 amino acid insert between residues 63 and 64 of the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90056-9
更新日期:1983-03-01 00:00:00
abstract::Spatiotemporal changes in nuclear lamina composition underlie cell-type-specific chromatin organization and cell fate, suggesting that the lamina forms a dynamic framework critical for genome function, cellular identity, and developmental potential. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.02.052
更新日期:2013-03-14 00:00:00
abstract::Secreted proteins of the Wnt family have profound organizing roles during animal development and are transduced via the activities of the Frizzled (Fz) class of transmembrane receptors and the TCF/LEF/Pangolin class of transcription factors. beta-catenins, including Drosophila Armadillo (Arm), link activation of Fz at...
journal_title:Cell
pub_type: 杂志文章,收录出版
doi:10.1016/s0092-8674(02)01037-1
更新日期:2002-10-18 00:00:00
abstract::The erythropoietin receptor (EpoR) is essential for production of red blood cells; a principal function of EpoR is to rescue committed erythroid progenitors from apoptosis. Stat5 is rapidly activated following EpoR stimulation, but its function in erythropoiesis has been unclear since adult Stat5a-/-5b-/- mice have no...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81013-2
更新日期:1999-07-23 00:00:00
abstract::Double-strand break (DSB) repair is critical for maintaining genomic integrity and requires the processing of the 5' DSB ends. Recent studies have shed light on the mechanism and regulation of DNA end processing during DSB repair by homologous recombination. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2009.05.007
更新日期:2009-05-29 00:00:00
abstract::The ligand for osteoprotegerin has been identified, and it is a TNF-related cytokine that replaces the requirement for stromal cells, vitamin D3, and glucocorticoids in the coculture model of in vitro osteoclastogenesis. OPG ligand (OPGL) binds to a unique hematopoeitic progenitor cell that is committed to the osteocl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81569-x
更新日期:1998-04-17 00:00:00
abstract::The light-sensing organelle of the vertebrate rod photoreceptor, the outer segment (OS), is a modified cilium containing approximately 1,000 stacked disc membranes that are densely packed with visual pigment rhodopsin. The mammalian OS is renewed every ten days; new discs are assembled at the base of the OS by a poorl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.06.030
更新日期:2007-08-10 00:00:00
abstract::Sixty-one codons specify 20 amino acids, offering cells many options for encoding a polypeptide sequence. Two new studies (Cannarrozzi et al., 2010; Tuller et al., 2010) now foster the idea that patterns of codon usage can control ribosome speed, fine-tuning translation to increase the efficiency of protein synthesis....
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2010.03.033
更新日期:2010-04-16 00:00:00
abstract::The role of electricity in biological systems was first appreciated through electrical stimulation experiments performed by Luigi Galvani in the 18th century. These pioneering experiments demonstrated that the behavior of living tissues is governed by the flow of electrochemical species-an insight that gave rise to th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.06.026
更新日期:2017-06-29 00:00:00
abstract::Human cerebral cortex size and complexity has increased greatly during evolution. While increased progenitor diversity and enhanced proliferative potential play important roles in human neurogenesis and gray matter expansion, the mechanisms of human oligodendrogenesis and white matter expansion remain largely unknown....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.06.027
更新日期:2020-08-06 00:00:00
abstract::Two dimensional gel electrophoresis (triton-acid-urea followed by SDS) has been used to resolve two previously uncharacterized, quantitatively minor histone variants in acid extracts from macronuclei of Tetrahymena thermophila. Utilizing techniques which allow characterization of these variants without purifying them ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90307-4
更新日期:1980-07-01 00:00:00
abstract::A mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step of transcription initiation, we used single-particle electron microscopy (EM) to visualize human TFIID with promoter DN...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.12.005
更新日期:2013-01-17 00:00:00
abstract::Certain tRNAs in S. cerevisiae (tRNATyr and tRNAPhe) arise via precursor molecules which are mature at the 5' and 3' termini but contain intervening sequences adjacent to the anticodon (Knapp et al., 1978; O'Farrell et al., 1978). In addition to these molecules, precursors to several other tRNAs accumulate in a temper...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90349-0
更新日期:1979-09-01 00:00:00
abstract::Protein-DNA binding is mediated by the recognition of the chemical signatures of the DNA bases and the 3D shape of the DNA molecule. Because DNA shape is a consequence of sequence, it is difficult to dissociate these modes of recognition. Here, we tease them apart in the context of Hox-DNA binding by mutating residues...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.02.008
更新日期:2015-04-09 00:00:00
abstract::Polycomb group (PcG) and trithorax group (trxG) proteins are critical regulators of numerous developmental genes. To silence or activate gene expression, respectively, PcG and trxG proteins bind to specific regions of DNA and direct the posttranslational modification of histones. Recent work suggests that PcG proteins...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2007.02.009
更新日期:2007-02-23 00:00:00
abstract::We constructed rabbit beta-globin genes with deletions in the large intron, extending from the midpoint toward the 5' or 3' splice sites. Analysis of transcripts in transformed HeLa cells showed that six 5' proximal intron nucleotides allowed normal splicing. Correct splicing at the 3' splice site required 12 or more ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90426-4
更新日期:1984-07-01 00:00:00
abstract::Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.08.027
更新日期:2014-10-09 00:00:00
abstract::Properties of human anticentromere autoantibodies were analyzed. In intact cells or isolated cell fractions, these sera stain the centromeres of mitotic chromosomes and discrete speckles (prekinetochores) in nuclei. Staining is also retained in matrix preparations from nuclei or chromosomes. Immunoprecipitation or imm...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90236-2
更新日期:1983-11-01 00:00:00
abstract::Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.11.020
更新日期:2021-01-07 00:00:00