Abstract:
:The initiation of transcription from the nitrogen-regulated promoter glnAp2 requires RNA polymerase containing sigma 54, the transcriptional activator NRI, and the protein kinase NRII, responsible for the conversion of NRI to the active NRI-phosphate. NRI-phosphate does not increase the ability of sigma 54-containing RNA polymerase to bind to the promoter, but rather stimulates the conversion of an initial promoter:polymerase complex to the transcriptionally active open complex. The presence on the DNA template of high-affinity binding sites for NRI/NRI-phosphate, normally located 130 and 100 bp upstream of the site of transcription initiation, results in a 4- to 5-fold lowering of the concentration of NRI required for the formation of the open complex. These high-affinity NRI binding sites facilitate open complex formation when they are moved to positions 700 bp further upstream or 950 bp downstream of glnAp2 on linear DNA templates.
journal_name
Celljournal_title
Cellauthors
Ninfa AJ,Reitzer LJ,Magasanik Bdoi
10.1016/0092-8674(87)90170-xsubject
Has Abstractpub_date
1987-09-25 00:00:00pages
1039-46issue
7eissn
0092-8674issn
1097-4172pii
0092-8674(87)90170-Xjournal_volume
50pub_type
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