Abstract：
:As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture of the Escherichia coli genome, showing its partition into two structurally distinct entities by a complex and intertwined network of contacts: the replication terminus (ter) region and the rest of the chromosome. Outside of ter, the condensin MukBEF and the ubiquitous nucleoid-associated protein (NAP) HU promote DNA contacts in the megabase range. Within ter, the MatP protein prevents MukBEF activity, and contacts are restricted to ∼280 kb, creating a domain with distinct structural properties. We also show how other NAPs contribute to nucleoid organization, such as H-NS, which restricts short-range interactions. Combined, these results reveal the contributions of major evolutionarily conserved proteins in a bacterial chromosome organization.
journal_name
Celljournal_title
Cellauthors
Lioy VS,Cournac A,Marbouty M,Duigou S,Mozziconacci J,Espéli O,Boccard F,Koszul Rdoi
10.1016/j.cell.2017.12.027subject
Has Abstractpub_date
2018-02-08 00:00:00pages
771-783.e18issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(17)31507-6journal_volume
172pub_type
杂志文章相关文献
CELL文献大全abstract：:Seeing begins in the photoreceptors, where light is absorbed and signaled to the nervous system. Throughout the animal kingdom, photoreceptors are diverse in design and purpose. Nonetheless, phototransduction-the mechanism by which absorbed photons are converted into an electrical response-is highly conserved and base...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2009.09.029
更新日期：2009-10-16 00:00:00
abstract：:Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2018.12.035
更新日期：2019-01-24 00:00:00
abstract：:Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90517-6
更新日期：1980-02-01 00:00:00
abstract：:Two decades of research identified more than a dozen clock genes and defined a biochemical feedback mechanism of circadian oscillator function. To identify additional clock genes and modifiers, we conducted a genome-wide small interfering RNA screen in a human cellular clock model. Knockdown of nearly 1000 genes reduc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.08.031
更新日期：2009-10-02 00:00:00
abstract：:Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.03.001
更新日期：2017-03-23 00:00:00
abstract：:Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.10.043
更新日期：2006-12-29 00:00:00
abstract：:Murine intracisternal A particles have a number of properties which are common to known RNA tumor viruses, but horizontal transmission has not been previously demonstrated. The apparent absence of infectivity may be related to the failure of these particles to be released from cisternae of endo-plasmic reticulum. Prev...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90083-0
更新日期：1976-11-01 00:00:00
abstract：:Removal of histone H1 produces a transition in the structure of chromatin fibers as observed by electron microscopy. Chromatin containing all histone proteins appears as fibers with a diameter of about 250 A. The nucleosomes within these fibers are closely packed. If histone H1 is selectively removed with 50-100 mM Na...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90188-x
更新日期：1977-09-01 00:00:00
abstract：:A mutant in src, the oncogene of Rous sarcoma virus, has been constructed in which the major phosphorylated tyrosine (Tyr-416, located in the carboxy-terminal half of the protein) has been replaced by phenylalanine. Mouse cells transformed with this mutant src form foci and grow in soft agar, indicative of a transform...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90074-0
更新日期：1983-03-01 00:00:00
abstract：:Immune responses in diverse tissue sites are critical for protective immunity and homeostasis. Here, we investigate how tissue localization regulates the development and function of human natural killer (NK) cells, innate lymphocytes important for anti-viral and tumor immunity. Integrating high-dimensional analysis of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.01.022
更新日期：2020-02-20 00:00:00
abstract：:In this issue of Cell, Lee et al. (2006) report that the parasite Trypanosoma brucei synthesizes fatty acids in an unconventional way. T. brucei and two other trypanosomes use enzymes called elongases to synthesize myristate, a fourteen carbon (C14) fatty acid essential for pathogenesis. This is an unexpected finding ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2006.08.001
更新日期：2006-08-25 00:00:00
abstract：:We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90028-3
更新日期：1981-10-01 00:00:00
abstract：:A family of novel substrates was designed to enable the efficient accumulation of intermediates in site-specific recombination. Strategically placed nicks allow these "suicide substrates" to initiate the reaction but prevent its completion or reversal. Consequently, it has been possible to determine that lambda site-s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90336-9
更新日期：1987-08-28 00:00:00
abstract：:Polycomb group proteins control a hierarchy of gene expression during the differentiation of stem cells. In this issue, Ezhkova et al. (2009) show that repression mediated by Polycomb proteins controls the timing of differentiation of precursor cells of the epidermal lineage. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.03.004
更新日期：2009-03-20 00:00:00
abstract：:We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-inter...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80024-6
更新日期：1985-06-01 00:00:00
abstract：:The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90149-3
更新日期：1995-12-29 00:00:00
abstract：:We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90098-j
更新日期：1991-05-17 00:00:00
abstract：:Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight into how nucleosomes are arranged in vivo, we combined quantitative super-resolution nanoscopy with computer simulations to visualize and count nucleosomes along the chromatin fiber in single nuclei. Nucleosomes assembled in heteroge...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.01.054
更新日期：2015-03-12 00:00:00
abstract：:Zika virus (ZIKV) is associated with severe neuropathology in neonates as well as Guillain-Barré syndrome and other neurologic disorders in adults. Prolonged viral shedding has been reported in semen, suggesting the presence of anatomic viral reservoirs. Here we show that ZIKV can persist in cerebrospinal fluid (CSF) ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.04.008
更新日期：2017-05-04 00:00:00
abstract：:Newly synthesized proteins are believed to move from the endoplasmic reticulum (ER) to the Golgi by bulk flow, and sorting is assumed to occur exclusively in the trans-Golgi network (TGN). Using quantitative immunoelectron microscopy, we demonstrate that vesicular stomatitis virus glycoprotein (VSV-G) is sorted from r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90359-x
更新日期：1994-03-11 00:00:00
abstract：:For many scientific awards, such as Nobels and Laskers, the maximum number of recipients is three. This Rule of Three forces selection committees to make difficult decisions that increase the likelihood of singling out those individuals who open a new field and continue to lead it. The Rule of Three is reminiscent of ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.08.040
更新日期：2016-09-22 00:00:00
abstract：:Two dimensional gel electrophoresis (triton-acid-urea followed by SDS) has been used to resolve two previously uncharacterized, quantitatively minor histone variants in acid extracts from macronuclei of Tetrahymena thermophila. Utilizing techniques which allow characterization of these variants without purifying them ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90307-4
更新日期：1980-07-01 00:00:00
abstract：:Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 def...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.03.036
更新日期：2012-05-11 00:00:00
abstract：:Fluorescent proteins are commonly used to label cells across organisms, but the unmodified forms cannot control biological activities. Using GFP-binding proteins derived from Camelid antibodies, we co-opted GFP as a scaffold for inducing formation of biologically active complexes, developing a library of hybrid transc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.07.021
更新日期：2013-08-15 00:00:00
abstract：:The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00191-0
更新日期：2003-03-21 00:00:00
abstract：:Misfolded proteins accumulating in several neurodegenerative diseases (including Alzheimer, Parkinson, and Huntington diseases) can cause aggregation of their native counterparts through a mechanism similar to the infectious prion protein's induction of a pathogenic conformation onto its cellular isoform. Evidence for...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.10.011
更新日期：2011-10-28 00:00:00
abstract：:The asymmetries of internal organs are consistently oriented along the left-right axis in all vertebrates, and perturbations of left-right orientation lead to significant congenital disease. We propose a model in which a "left-right coordinator" interacts with the Spemann organizer to coordinate the evolutionarily con...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81144-7
更新日期：1998-04-03 00:00:00
abstract：:Bovine Papilloma Virus (BPV-1) establishes itself as a multicopy nuclear plasmid in somatic mammalian cells in culture. We report here that two discontinuous regions within the viral genome can independently support extrachromosomal replication of the Tn5 neomycinr gene in cells that provide viral factors in trans. Th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90232-0
更新日期：1984-02-01 00:00:00
abstract：:Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse thoracic duct (TD) lymph contains HSPCs that possess short- and lo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.09.047
更新日期：2007-11-30 00:00:00
abstract：:Overlapping cDNA clones from the translocation breakpoint region (TBR) gene, recently discovered at the neurofibromatosis type 1 locus and found to be interrupted by deletions and a t(17;22) translocation, have been sequenced. A 4 kb sequence of the transcript of the TBR gene has been compared with sequences of genomi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90253-b
更新日期：1990-07-13 00:00:00
