Abstract：
:Murine intracisternal A particles have a number of properties which are common to known RNA tumor viruses, but horizontal transmission has not been previously demonstrated. The apparent absence of infectivity may be related to the failure of these particles to be released from cisternae of endo-plasmic reticulum. Previous biological studies using isolated, purified A particles have been compromised by the fact that the isolation procedure requires small amounts of nonionic detergent. Using some techniques of somatic cell hybridization, we have assessed the capacity for A particle genome transfer from positive to negative cells. Since it has been previously shown that some chloramphenicol-resistant cell lines can transfer this resistance in the cytoplasm, we have used this characteristic as a marker for cytoplasmic fragments. Mouse cells containing A particles were mutagenized, and clones resistant to chloramphenicol were selected; by enucleating these cells and fusing the resultant cytoplasts to each of two recipient mouse cell lines negative for A particles, it is possible to identify clones of cells known to be the product of a fusion event between a cytoplast and a whole cell (cybrids). Under these conditions, intracisternal A particles appear in the cybrid clones as a phenotypic trait that has not been segregated over at least 60-80 cell generations.
journal_name
Celljournal_title
Cellauthors
Malech HL,Wivel NAdoi
10.1016/0092-8674(76)90083-0subject
Has Abstractpub_date
1976-11-01 00:00:00pages
383-91issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(76)90083-0journal_volume
9pub_type
杂志文章相关文献
CELL文献大全abstract：:Many proteins contain disordered regions of low-sequence complexity, which cause aging-associated diseases because they are prone to aggregate. Here, we study FUS, a prion-like protein containing intrinsically disordered domains associated with the neurodegenerative disease ALS. We show that, in cells, FUS forms liqui...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.07.047
更新日期：2015-08-27 00:00:00
abstract：:Factors controlling the onset and progression of extracellular amyloid diseases remain largely unknown. Central to disease etiology is the efficiency of the endoplasmic reticulum (ER) machinery that targets destabilized mutant proteins for degradation and the enhanced tendency of these variants to aggregate if secrete...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.01.018
更新日期：2005-04-08 00:00:00
abstract：:Compared to sperm nuclei, nuclei from adult somatic cells replicate inefficiently in frog egg extract. In this issue of Cell, Lemaitre et al. (2005) show that pre-exposure of erythrocyte nuclei to a mitotic extract removes this difference, reorganizes the chromatin into shorter loops, and allows replication at much sh...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2005.11.008
更新日期：2005-12-02 00:00:00
abstract：:Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified transmembrane protein found in CNS myelin and testis with unknown function. Herein we demonstrate that Osp null mice exhibit both neurological and reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is conspicuous, and male...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81553-6
更新日期：1999-12-10 00:00:00
abstract：:Activation of plasma membrane calcium channels by depletion of endoplasmic reticulum (ER) calcium stores is important for calcium entry in many cell types. Park et al. (2009) now show that direct binding of the ER protein STIM to tetramers of the Orai1 calcium channel in the plasma membrane triggers opening of this ch...
journal_title：Cell
pub_type： 评论,社论
doi：10.1016/j.cell.2009.02.022
更新日期：2009-03-06 00:00:00
abstract：:We have established the DNA sequence of nine yeast mutants that prevent the expression either of the split cytochrome b gene alone (five mutants) or of two split genes, the cytochrome b gene and the cytochrome oxidase subunit I gene (four mutants). All the mutations analyzed are localized in intron 14 of the cob-box g...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90051-4
更新日期：1982-04-01 00:00:00
abstract：:This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90026-6
更新日期：1979-03-01 00:00:00
abstract：:Metabolic activity is intimately linked to T cell fate and function. Using high-resolution mass spectrometry, we generated dynamic metabolome and proteome profiles of human primary naive T cells following activation. We discovered critical changes in the arginine metabolism that led to a drop in intracellular L-argini...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.031
更新日期：2016-10-20 00:00:00
abstract：:H2S is an endogenous gasotransmitter that plays an important role in physiological conditions. In this issue, Das et al. provide evidence that SIRT1-dependent angiogenesis is augmented by H2S-findings reinforced by Longchamp et al., who demonstrate that H2S-dependent angiogenesis is triggered by amino acid deprivation...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2018.03.011
更新日期：2018-03-22 00:00:00
abstract：:Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culmina...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80405-5
更新日期：1997-10-17 00:00:00
abstract：:During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90076-b
更新日期：1991-11-15 00:00:00
abstract：:The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-paren...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.09.012
更新日期：2020-10-29 00:00:00
abstract：:In prokaryotes, the toxin-antitoxin systems are thought to play important roles in growth regulation under stress conditions. In the E. coli MazE-MazF system, MazF toxin functions as an mRNA interferase cleaving mRNAs at ACA sequences to inhibit protein synthesis leading to cell growth arrest. Myxococcus xanthus is a ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.11.044
更新日期：2008-01-11 00:00:00
abstract：:Neuronal plasticity depends on plasma membrane Ca(2+) influx, resulting in activity-dependent gene transcription. Calmodulin (CaM) activated by Ca(2+) initiates the nuclear events, but how CaM makes its way to the nucleus has remained elusive. Ma et al. now show that CaMKIIγ transports CaM from cell surface Ca(2+) cha...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2014.09.023
更新日期：2014-10-09 00:00:00
abstract：:Inflammation is associated with many neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and multiple sclerosis. In this Review, we discuss inducers, sensors, transducers, and effectors of neuroinflammation that contribute to neuronal dysfunction and death. Al...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2010.02.016
更新日期：2010-03-19 00:00:00
abstract：:c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90663-b
更新日期：1993-01-29 00:00:00
abstract：:The physical state of the viral genome in four lines of hamster cells transformed by adenovirus type 12 (Ad12) has been investigated. The four lines of transformed cells originated from hamster cells after infection with Ad12 at multiplicities ranging from 5-350 plaque-forming units per cell. The DNA from transformed ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90144-1
更新日期：1977-01-01 00:00:00
abstract：:DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90788-5
更新日期：1989-06-02 00:00:00
abstract：:Zika virus (ZIKV) is associated with severe neuropathology in neonates as well as Guillain-Barré syndrome and other neurologic disorders in adults. Prolonged viral shedding has been reported in semen, suggesting the presence of anatomic viral reservoirs. Here we show that ZIKV can persist in cerebrospinal fluid (CSF) ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.04.008
更新日期：2017-05-04 00:00:00
abstract：:Vg1 is a TGF beta-related growth factor encoded by a maternal mRNA localized to vegetal blastomeres in Xenopus embryos. Vg1 precursor protein is abundant in vegetal cells, but the processed mature form has not been readily detected and no activity has been demonstrated for the putative Vg1 mature protein. We have engi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)80045-g
更新日期：1993-08-13 00:00:00
abstract：:In this issue of Cell, two articles show that tumor-specific changes in HLA-mediated antigen presentation affect tumor immunogenicity and may play a role in shaping cancer cell survival. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2017.11.012
更新日期：2017-11-30 00:00:00
abstract：:Genetic alterations in signaling pathways that control cell-cycle progression, apoptosis, and cell growth are common hallmarks of cancer, but the extent, mechanisms, and co-occurrence of alterations in these pathways differ between individual tumors and tumor types. Using mutations, copy-number changes, mRNA expressio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.03.035
更新日期：2018-04-05 00:00:00
abstract：:The use of optogenetic approaches has revealed new roles for intracellular protein condensates described in two papers in this issue of Cell (Bracha et. al., 2018; Shin et al., 2018). These results show that growing condensates are able to exert mechanical forces resulting in chromatin rearrangement, establishing a ne...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2018.11.020
更新日期：2018-11-29 00:00:00
abstract：:ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.09.032
更新日期：2012-10-26 00:00:00
abstract：:We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA deg...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00065-9
更新日期：2000-09-15 00:00:00
abstract：:Mammalian models of longevity are related primarily to caloric restriction and alterations in metabolism. We examined mice in which type 5 adenylyl cyclase (AC5) is knocked out (AC5 KO) and which are resistant to cardiac stress and have increased median lifespan of approximately 30%. AC5 KO mice are protected from red...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.05.038
更新日期：2007-07-27 00:00:00
abstract：:32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90318-x
更新日期：1977-05-01 00:00:00
abstract：:Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birt...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.05.052
更新日期：2014-08-14 00:00:00
abstract：:Inositol phosphates are well-known signaling molecules, whereas the inositol pyrophosphates, such as diphosphoinositol pentakisphosphate (InsP7/IP7) and bis-diphosphoinositol tetrakisphosphate (InsP8/IP8), are less well characterized. We demonstrate physiologic regulation of Dictyostelium chemotaxis by InsP7 mediated ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00640-8
更新日期：2003-09-05 00:00:00
abstract：:The E. coli replication fork synthesizes DNA at the rate of nearly 1000 nt/s. We show here that an interaction between the tau subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork DNA helicase (DnaB) is required to mediate this high rate of replication fork movement. In th...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81039-9
更新日期：1996-02-23 00:00:00
